抗gp130抗体(Anti-gp130, Mouse, Rat-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse gp130Gln23-Glu617 (predicted)Accession # Q6PDI9
Source	Monoclonal Rat IgG2A Clone # 125623
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse gp130 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human gp130 is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Flow Cytometry
	Detection of gp130 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE‑conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse gp130 Monoclonal Antibody (Catalog # MAB4681) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
Immunocytochemistry
	gp130 in M1 Mouse Cell Line. gp130 was detected in immersion fixed M1 mouse myeloid leukemia cell line using Rat Anti-Mouse gp130 Monoclonal Antibody (Catalog # MAB4681) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Long Name	Glycoprotein 130
Entrez Gene IDs	3572 (Human); 16195 (Mouse); 25205 (Rat)
Background	gp130
background_content	Background: gp130 Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R. Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. The in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.

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Citations

1. Granulocytes Are Unresponsive to IL-6 Due to an Absence of gp130
   Authors: AN Wilkinson, KH Gartlan, G Kelly, LD Samson, SD Olver, J Avery, N Zomerdijk, SK Tey, JS Lee, S Vuckovic, GR Hill
   J. Immunol., 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Dendritic cell MST1 inhibits Th17 differentiation
   Authors: C Li, Y Bi, Y Li, H Yang, Q Yu, J Wang, Y Wang, H Su, A Jia, Y Hu, L Han, J Zhang, S Li, W Tao, G Liu
   Nat Commun, 2017;8(0):14275.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. Epstein-Barr virus-induced gene 3 (EBI3) can mediate IL-6 trans-signaling
   Authors: S Chehboun, J Labrecque-, S Pasquin, Y Meliani, B Meddah, W Ferlin, M Sharma, A Tormo, JF Masson, JF Gauchat
   J. Biol. Chem, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


4. Oncostatin M acting via OSMR, augments the actions of IL-1 and TNF in synovial fibroblasts.
   Authors: Le Goff B, Singbrant S, Tonkin B, Martin T, Romas E, Sims N, Walsh N
   Cytokine, 2014;68(2):101-9.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


5. Ifitm3 limits the severity of acute influenza in mice.
   Authors: Bailey, Charles, Huang, I-Chueh, Kam, Christin, Farzan, Michael
   PLoS Pathog, 2012;8(9):e1002909.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


6. Essential roles of IL-6 trans-signaling in colonic epithelial cells, induced by the IL-6/soluble-IL-6 receptor derived from lamina propria macrophages, on the development of colitis-associated premalignant cancer in a murine model.
   Authors: Matsumoto S, Hara T, Mitsuyama K, Yamamoto M, Tsuruta O, Sata M, Scheller J, Rose-John S, Kado S, Takada T
   J. Immunol., 2010;184(3):1543-51.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


7. TH and NPY in sympathetic neurovascular cultures: role of LIF and NT-3.
   Am. J. Physiol., Cell Physiol., 2007;294(1):C306-12.
   Species: Rat
   Sample Type: Cell Lysates
   Application: WB

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