抗Human CD163抗体(Anti-Human CD163 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human CD163Gly46-Ser1050Accession # Q86VB7
Source	Monoclonal Mouse IgG1 Clone # 215927
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human CD163 in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human CD163 (Catalog # 1607-CD) under non-reducing conditions only
Flow Cytometry	2.5 µg/106 cells	Human whole blood monocytes
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

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Related Product & Information

Background

CD163

background_content

Background: CD163 CD163, previously called M130 or p155, is a 130‑160 kDa type I transmembrane glycoprotein that belongs to group B of the cysteine-rich scavenger receptor family (1‑3). It is essential for clearance of hemoglobin-haptoglobin (Hb-Hp) complexes in the liver, spleen and circulation (4). The human CD163 contains a 41 amino acid (aa) signal sequence, a 1009 aa extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 22 aa transmembrane segment, and a 39‑84 aa cytoplasmic region (1). The third SRCR domain is crucial for calcium-dependent binding of hemoglobin/haptoglobin complexes (3). Three splice forms (isoforms 2, 3 and 4) vary within their intracellular regions (1, 5), while one isoform (# 4) also has a 34 aa insert between SRCR domains 5 and 6 within the ECD. While all are expressed, isoform 3 is the most abundant, being generally expressed on the cell surface and most active in endocytosis (5). An approximately 130 kDa soluble form of human CD163 (sCD163) is assumed to contain virtually all of the ECD, which shares 74%, 75%, 84%, 86%, 86% and 87% aa identity with mouse, rat, bovine, equine, porcine and canine CD163 ECD, respectively (6, 7). It is released from the cell surface by proteolysis after oxidative stress or inflammatory stimuli, including bacterial endotoxins and activation of the Toll-like receptors TLR2 or TLR5 (7‑10). Expression of CD163 is constitutive, and induced by glucocorticoids, IL‑10, IL‑6 or endotoxin on circulating monocytes, tissue macrophages, and at low levels on monocyte-derived dendritic cells (1, 2, 11, 12). In addition to clearing Hb‑Hp complexes, CD163 is also a scavenger receptor for free Hb (if Hp is depleted) and TWEAK (TNF-like weak inducer of apoptosis), and can function as an erythroblast adhesion receptor (4, 13‑15).

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Citations

1. Human fetal dendritic cells promote prenatal T-cell immune suppression through arginase-2
   Authors: N McGovern, A Shin, G Low, D Low, K Duan, LJ Yao, R Msallam, I Low, NB Shadan, HR Sumatoh, E Soon, J Lum, E Mok, S Hubert, P See, EH Kunxiang, YH Lee, B Janela, M Choolani, CNZ Mattar, Y Fan, TKH Lim, DK Hong, KK Tan, JKC Tam, C Schuster, A Elbe-Bürge, XN Wang, V Bigley, M Collin, M Haniffa, A Schlitzer, M Poidinger, S Albani, A Larbi, EW Newell, JKY Chan, F Ginhoux
   Nature, 2017;0(0):.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: Flow


2. Jagged1 Instructs Macrophage Differentiation in Leprosy
   PLoS Pathog, 2016;12(8):e1005808.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Endoneurial macrophages induce perineural invasion of pancreatic cancer cells by secretion of GDNF and activation of RET tyrosine kinase receptor.
   Authors: Cavel O, Shomron O, Shabtay A, Vital J, Trejo-Leider L, Weizman N, Krelin Y, Fong Y, Wong R, Amit M, Gil Z
   Cancer Res, 2012;72(22):5733-43.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC Fresh


4. Glioma-associated cytomegalovirus mediates subversion of the monocyte lineage to a tumor propagating phenotype.
   Authors: Dziurzynski K, Wei J, Qiao W, Hatiboglu MA, Kong LY, Wu A, Wang Y, Cahill D, Levine N, Prabhu S, Rao G, Sawaya R, Heimberger AB
   Clin. Cancer Res., 2011;17(14):4642-9.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Human uterine epithelial cell secretions regulate dendritic cell differentiation and responses to TLR ligands.
   Authors: Ochiel DO, Ghosh M, Fahey JV
   J. Leukoc. Biol., 2010;88(3):435-44.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. The class A scavenger receptor, macrophage receptor with collagenous structure, is the major phagocytic receptor for Clostridium sordellii expressed by human decidual macrophages.
   Authors: Thelen T, Hao Y, Medeiros AI
   J. Immunol., 2010;185(7):4328-35.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. Generation of mature human myelomonocytic cells through expansion and differentiation of pluripotent stem cell-derived lin-CD34+CD43+CD45+ progenitors.
   Authors: Choi KD, Vodyanik MA, Slukvin II
   J. Clin. Invest., 2009;119(9):2818-29.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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