抗Human VEGF R2/KDR抗体(Anti-Human VEGF R2/KDR antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF R2Ala20-Glu764Accession # P35968
Source	Monoclonal Mouse IgG1 Clone # 89115
Purification	Protein A or G purified from ascites
Specificity	Detects human VEGF R2 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) VEGF R1, rhVEGF R3, or recombinant mouse VEGF R2.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human VEGF R2/KDR/Flk-1 Fc Chimera (Catalog # 357-KD) under non-reducing conditions only. It is recommended to use Goat Anti-Human VEGF R2/KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357)
Immunohistochemistry	8-25 µg/mL	See below
Immunocytochemistry	5-25 µg/mL	See below

Immunohistochemistry
	VEGF R2/KDR/Flk‑1 in Human Placenta. VEGF R2/KDR/Flk‑1 was detected in immersion fixed paraffin-embedded sections of human placenta using 25 µg/mL Human VEGF R2/KDR/Flk‑1 Monoclonal Antibody (Catalog # MAB3571) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
	VEGF R2/KDR/Flk‑1 in Human Kidney. VEGF R2/KDR/Flk‑1 was detected in immersion fixed paraffin-embedded sections of human kidney array using Human VEGF R2/KDR/Flk‑1 Monoclonal Antibody (Catalog # MAB3571) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunocytochemistry
	VEGF R2/KDR/Flk‑1 in HUVECs and Human HeLa Cell Line. VEGF R2/KDR/Flk‑1 was detected in immersion fixed human umbilical vein endothelial cells (HUVECs; left panel) but is not detected in Hela human cervical epithelial carcinoma cell line (right panel) using Mouse Anti-Human VEGF R2/KDR/Flk‑1 Monoclonal Antibody (Catalog # MAB3571) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of HUVECs. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Background

VEGF R2/KDR/Flk-1

background_content

Background: VEGF R2/KDR/Flk-1 VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. VEGF R2 cDNA encodes a 1356 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGF R2 is composed of a 745 aa residue extracellular domain, a 25 aa residue transmembrane domain and a 567 aa residue cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGF R2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

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Citations

1. Delivery of sodium morrhuate to hemangioma endothelial cells using immunoliposomes conjugated with anti-VEGFR2/KDR antibody
   Authors: X Li, X Ren, J Liang, W Ma, Z Wang, Z Yang
   Int J Nanomedicine, 2017;12(0):6963-6972.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


2. Endothelial progenitor cells from human fetal aorta cure diabetic foot in a rat model
   Authors: Jin-Ning Lou
   Metab. Clin. Exp., 2016;65(12):1755-1767.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors.
   Authors: Niwa A, Heike T, Umeda K
   PLoS ONE, 2011;6(7):e22261.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


4. Tumour vascularization via endothelial differentiation of glioblastoma stem-like cells.
   Authors: Ricci-Vitiani L, Pallini R, Biffoni M, Todaro M, Invernici G, Cenci T, Maira G, Parati EA, Stassi G, Larocca LM, De Maria R
   Nature, 2010;468(7325):824-8.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


5. Adenoviral expression of vascular endothelial growth factor splice variants differentially regulate bone marrow-derived mesenchymal stem cells.
   Authors: Lin H, Shabbir A, Molnar M, Yang J, Marion S, Canty JM, Lee T
   J. Cell. Physiol., 2008;216(2):458-68.
   Species: Porcine
   Sample Type: Whole Cells
   Application: ICC


6. Development of anti-atherosclerotic tissue-engineered blood vessel by A20-regulated endothelial progenitor cells seeding decellularized vascular matrix.
   Authors: Zhu C, Ying D, Mi J, Li L, Zeng W, Hou C, Sun J, Yuan W, Wen C, Zhang W
   Biomaterials, 2008;29(17):2628-36.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


7. Thrombopoietin enhances generation of CD34+ cells from human embryonic stem cells.
   Authors: Srivastava AS, Nedelcu E, Esmaeli-Azad B, Mishra R, Carrier E
   Stem Cells, 2007;25(6):1456-61.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


8. Expression of VEGFR-2 on HaCaT cells is regulated by VEGF and plays an active role in mediating VEGF induced effects.
   Authors: Yang XH, Man XY, Cai SQ, Yao YG, Bu ZY, Zheng M
   Biochem. Biophys. Res. Commun., 2006;349(1):31-8.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


9. Isolation of renal progenitor cells from adult human kidney.
   Authors: Bussolati B, Bruno S, Grange C, Buttiglieri S, Deregibus MC, Cantino D, Camussi G
   Am. J. Pathol., 2005;166(2):545-55.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC

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