抗CD45抗体(Anti-CD45, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Human peripheral blood mononuclear cells
Source	Monoclonal Mouse IgG1 Clone # 2D1
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human CD45. This antibody recognizes all isoforms of human CD45.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	See below
Immunohistochemistry	5-25 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Flow Cytometry
	Detection of CD45 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD45 Monoclonal Antibody (Catalog # MAB1430, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Immunocytochemistry
	CD45 in Human PBMCs. CD45 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA blast using Mouse Anti-Human CD45 Monoclonal Antibody (Catalog # MAB1430) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunohistochemistry
	CD45 in Hodgkin's Lymphoma Tissue. CD45 was detected in immersion fixed paraffin-embedded sections of Hodgkins lymphoma tissue using Mouse Anti-Human CD45 Monoclonal Antibody (Catalog # MAB1430) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Related Product & Information

Background

CD45

background_content

Background: CD45 CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mice, is member C of the class 1 (receptor‑like) protein tyrosine phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180‑220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1‑3). CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure (1‑5). The longest form, CD45RABC (called B220 in mouse), is expressed on B lymphocytes (5). The CD45RABC cDNA encodes 1304 amino acids (aa), including a 23 aa signal sequence, a 552 aa extracellular domain containing the splicing region, a cysteine‑rich region and two fibronectin type III domains, a 22 aa transmembrane sequence, and a 707 aa cytoplasmic domain that contains two phosphatase domains, D1 and D2. Only D1 has phosphatase activity. CD45R0 is the shortest form, lacking exons 4, 5 and 6 which encode aa 32‑191. It is expressed on memory cells, while intermediate sizes are expressed on other T cells (3, 4, 6). CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6‑8). CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Galectin interaction can be fine‑tuned by varying usage of the heavily O‑glycosylated spliced regions and sialylation of N‑linked carbohydrates (4, 9). Within the IS, CD45 dephosphorylates and negatively regulates the Src family kinase, Lck (8‑10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP‑1/CD148 (11‑14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).

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Citations

1. Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model
   Authors: JR Fan, HT Lee, W Lee, CH Lin, CY Hsu, CH Hsieh, WC Shyu
   Cell Death Dis, 2018;9(5):502.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC-F


2. Preferential Lineage-Specific Differentiation of Osteoblast-Derived Induced Pluripotent Stem Cells into Osteoprogenitors
   Authors: CL Roberts, SS Chen, AC Murchison, RA Ogle, MP Francis, RC Ogle, PC Sachs
   Stem Cells Int, 2017;2017(0):1513281.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Proteomic profiling of peripheral blood neutrophils identifies two inflammatory phenotypes in stable COPD patients
   Authors: ALT Loi, S Hoonhorst, C van Aalst, J Langereis, V Kamp, S Sluis-Eisi, N Ten Hacken, JW Lammers, L Koenderman
   Respir. Res., 2017;18(1):100.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Regional Differences Between Perisynovial and Infrapatellar Adipose Tissue Depots and Their Response to Class II and III Obesity in Patients with OA
   Authors: NS Harasymowi, ND Clement, A Azfer, R Burnett, DM Salter, AH Simpson
   Arthritis & rheumatology (Hoboken, N.J.), 2017;0(0):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


5. Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke
   Stem Cells Int, 2016;2016(0):6104780.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Switch from canonical to noncanonical Wnt signaling mediates high glucose-induced adipogenesis.
   Authors: Keats E, Dominguez J, Grant M, Khan Z
   Stem Cells, 2014;32(6):1649-60.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


7. Isolation and characterization of synovial mesenchymal stem cells.
   Authors: Harvanova D, Tothova T, Sarissky M, Amrichova J, Rosocha J
   Folia Biol. (Praha), 2011;57(3):119-24.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC

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