抗CSF-1 Receptor抗体(Anti-CSF-1 Receptor, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human M-CSF R/CD115Ile20-Glu512 (Pro54Ala)Accession # P07333.2
Source	Monoclonal Mouse IgG1 Clone # 61708
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human M-CSF R/CD115 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) GM-CSF R alpha orrhGM-CSF R beta.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	Human peripheral blood monocytes
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

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Related Product & Information

Background

M-CSF R/CD115

background_content

Background: M-CSF R/CD115 M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1‑4). M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta (1). Human M-CSF receptor cDNA encodes a 972 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 493 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain, and a 435 aa cytoplasmic domain. The human M-CSF R ECD shares 60%, 64%, 72%, 75%, 75%, and 76% aa identity with mouse, rat, bovine, canine, feline, and equine M-CSF R, respectively. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK, and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts and DC (3, 4). M-CSF R and integrin  alpha v beta 3 share signaling pathways during osteoclastogenesis and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10).

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Citations

1. Imatinib and Nilotinib Off-Target Effects on Human NK Cells, Monocytes, and M2 Macrophages
   Authors: F Bellora, A Dondero, MV Corrias, B Casu, S Regis, F Caliendo, A Moretta, M Cazzola, C Elena, L Vinti, F Locatelli, C Bottino, R Castriconi
   J. Immunol., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Direct inhibition of human RANK+ osteoclast precursors identifies a homeostatic function of IL-1beta.
   Authors: Lee B, Kim TH, Jun JB, Yoo DH, Woo JH, Choi SJ, Lee YH, Song GG, Sohn J, Park-Min KH, Ivashkiv LB, Ji JD
   J. Immunol., 2011;185(10):5926-34.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Resistance of acute myeloid leukemic cells to the triterpenoid CDDO-Imidazolide is associated with low caspase-8 and FADD levels.
   Authors: Riccioni R, Senese M, Diverio D, Riti V, Mariani G, Boe A, LoCoco F, Foa R, Peschle C, Sporn M, Testa U
   Leuk. Res., 2008;32(8):1244-58.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts.
   Authors: Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foa R, Peschle C, Testa U
   Stem Cells, 2007;25(8):1862-71.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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