抗IL-17B Receptor抗体(Anti-IL-17B Receptor, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human IL‑17 RBArg18-Gly289Accession # Q9NRM6
Source	Monoclonal Mouse IgG2B Clone # 170220
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL‑17 RB in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) IL‑17 RB, recombinant human IL-17 R, or rmIL-17 R is observed.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human IL‑17B R Fc Chimera (Catalog # 1207-BR)
Flow Cytometry	2.5 µg/106 cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry
	Detection of IL‑17 RB in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human IL‑17 RB Monoclonal Antibody (Catalog # MAB1207, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

目次に戻る

Related Product & Information

Background

IL-17 RB

background_content

Background: IL-17 RB The interleukin 17 (IL-17) family of cytokines, comprising six members (IL-17, IL-17B through IL-17F), are structurally related proteins with a conserved cysteine-knot structure. These proinflammatory cytokines can induce local cytokine productions and are involved in the regulation of the immune response. The cognate receptors activated by some of these cytokines have been identified (1). Interleukin-17 B Receptor (IL-17 RB), also known as IL-17Rh1, IL-17ER and EVI27, represents the second receptor of the IL-17 family of cytokines to be recognized (2-4). Human IL-17 RB cDNA encodes a 502 amino acid (aa) residue type I membrane protein with a putative 17 aa signal peptide, a 275 aa extracellular domain, a 21 aa transmembrane domain and a 189 aa cytoplasmic tail. By alternative splicing, a secreted variant of IL-17 RB has also been identified (4). Human and mouse IIL-17 RB share 76% aa sequence identity. The human IL-17 RB protein sequence is only 19.2% identical to the human IL-17 R sequence, but the two receptors share many conserved cysteine residues within their extracellular domains as well as additional conserved elements within their cytoplasmic domains. Three additional type I transmembrane receptors with homology to IL-17 R have been reported, increasing the number of the IL-17 R family members to five (5, 6). By Northern blot analysis, human IL-17 RB is highly expressed in kidneys and liver but is expressed at lower levels in testes, brain, small intestine and other endocrine tissues (2-4). The expression of IL-17 RB is significantly up-regulated under inflammatory conditions. IL-17 RB binds strongly to IL-17E and weakly to IL-17B. It does not bind IL-17, IL-17C, and IL-17F. Activation of IL-17 RB by its ligands results in the activation of nuclear factor kappa-B (2-4).

目次に戻る

Citations

1. IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs
   Authors: SM Bal, JH Bernink, M Nagasawa, J Groot, MM Shikhagaie, K Golebski, CM van Drunen, R Lutter, RE Jonkers, P Hombrink, M Bruchard, J Villaudy, JM Munneke, W Fokkens, JS Erjefält, H Spits, XR Ros
   Nat Immunol, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. IL-17RB(+) granulocytes are associated with airflow obstruction in asthma
   Authors: Lin Li
   Ann. Allergy Asthma Immunol, 2016;117(6):674-679.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. IL-25 in atopic dermatitis: a possible link between inflammation and skin barrier dysfunction?
   Authors: Hvid M, Vestergaard C, Kemp K
   J. Invest. Dermatol., 2011;131(1):150-7.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


4. Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells.
   Authors: Islam SA, Chang DS, Colvin RA
   Nat. Immunol., 2011;12(2):167-77.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. IL-17E upregulates the expression of proinflammatory cytokines in lung fibroblasts.
   Authors: Létuvé S, Lajoie-Kadoch S, Audusseau S, Rothenberg ME, Fiset PO, Ludwig MS, Hamid Q
   J. Allergy Clin. Immunol., 2006;117(3):590-6.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

目次に戻る