抗Human ErbB2/Her2抗体(Anti-Human ErbB2/Her2 antibody)
掲載日情報：2021/01/28 現在Webページ番号：29233
Human ErbB2/Her2に対する抗体(Anti-Human ErbB2/Her2 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2026年04月06日 18時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Human ErbB2/Her2 MAb (Clone 191924) |
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Anti-Human ErbB2/Her2 MAb (Clone 191924) |
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Anti-Human ErbB2/Her2 MAb (Clone 191924) |
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[在庫・価格 ：2026年04月06日 18時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human ErbB2/Her2 MAb (Clone 191924)
文献数： 7
- 商品コード：MAB1129-100
- メーカー：RSD
- 包装：100μg
- 価格：¥79,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別名：CD340 クローン：191924 Genbank No： 2064 Protein Accession No： P04626 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,FCM,IC,IHC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | |||||||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human ErbB2/Her2 MAb (Clone 191924)
文献数： 7
- 商品コード：MAB1129-500
- メーカー：RSD
- 包装：500μg
- 価格：¥266,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Human ErbB2/Her2 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF1129，スタンダードとして#1129-ER-050を用いる。 別名：CD340 クローン：191924 Genbank No： 2064 Protein Accession No： P04626 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,FCM,IC,IHC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | |||||||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human ErbB2/Her2 MAb (Clone 191924)
文献数： 7
- 商品コード：MAB1129-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥28,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：CD340 クローン：191924 Genbank No： 2064 Protein Accession No： P04626 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,FCM,IC,IHC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Unconjugated |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human ErbB2/Her2Thr23-Thr652Accession # P04626 |
| Source | Monoclonal Mouse IgG2B Clone # 191924 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human ErbB2/Her2 in ELISAs and Western blots. In ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) EGF R, rhErbB3, or rhErbB4. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | Recombinant Human ErbB2/Her2 Fc Chimera (Catalog # 1129-ER) under non-reducing conditions only |
| Flow Cytometry | 0.25 µg/106 cells | See below |
| Immunohistochemistry | 8-25 µg/mL | See below |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Immunocytochemistry | 8-25 µg/mL | See below |
| Human ErbB2/Her2 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human ErbB2/Her2 Antibody (Catalog #MAB1129 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human ErbB2/Her2 Biotinylated Antibody (Catalog #BAF1129 ) |
| ELISA Standard | Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF (Catalog #1129-ER ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Flow Cytometry |
![Flow Cytometry ErbB2/Her2 Antibody (191924) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/ErbB2_MAB1129_Flow_Cytometry_19910.jpg) click image to view larger | Detection of ErbB2/Her2 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human ErbB2/Her2 Monoclonal Antibody (Catalog # MAB1129, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins. |
| Immunocytochemistry |
![Immunocytochemistry ErbB2/Her2 Antibody (191924) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/ErbB2_MAB1129_Immunocytochemistry_9631.jpg) click image to view larger | ErbB2/Her2 in MDA‑MB‑231 Human Cell Line.ErbB2/Her2 was detected in immersion fixed MDA‑MB‑231 human breast cancer cell line using Mouse Anti-Human ErbB2/Her2 Monoclonal Antibody (Catalog # MAB1129) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
| Immunohistochemistry |
![Immunohistochemistry ErbB2/Her2 Antibody (191924) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/ErbB2_MAB1129_Immunohistochemistry_17193.jpg) click image to view larger | ErbB2/Her2 in Human Breast Cancer Tissue.ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human ErbB2/Her2 Monoclonal Antibody (Catalog # MAB1129) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
| Immunohistochemistry |
![Immunohistochemistry ErbB2/Her2 Antibody (191924) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/ErbB2_MAB1129_Immunohistochemistry_17345.jpg) click image to view larger | ErbB2/Her2 in Human Stomach. ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human ErbB2/Her2 Monoclonal Antibody (Catalog # MAB1129) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane/cytoplasm in glans and villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: ErbB2/Her2 | ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGF R), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation (1-10). | |
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References
| Coussens, L. et. al. (1985) Science 230:1132. | |
| Yamamoto, T. et. al. (1986) Nature 319:230. | |
| Kanai, Y. et. al. (1995) Biochem. Biophys. Res. Commun. 208:1067. | |
| Codony-Servat, J. et. al. (1999) Cancer Res. 59:1196. | |
| Carraway, K.L. 3rd et. al. (1994) J. Biol. Chem. 269:14303. | |
| Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172. | |
| Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859. | |
| Schlessinger, J. (2000) Cell 103:211. | |
| Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153. | |
| Daly, R.J. (1999) Growth Factors 16:255. | |
| Long Name: | Receptor Tyrosine Protein Kinase ErbB2 |
| Entrez Gene IDs: | 2064 (Human); 13866 (Mouse); 24337 (Rat) |
| Alternate Names: | CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC 2.7.10.1; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian) |
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Related Research Areas
| Cancer Biomarkers | ||||||||
| Cancer Stem Cell Markers | ||||||||
| Cytokine and Growth Factor Receptors on VSMC | ||||||||
| EGF Family | ||||||||
| Receptor Tyrosine Kinases (RTKs) | ||||||||
| Receptor Tyrosine Kinases (RTKs) in the Akt Pathway | ||||||||
| Receptors in the Jak/STAT Pathway | ||||||||
| Semaphorins, Plexin Receptors, and Related Molecules | ||||||||
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| Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||
|---|---|---|---|---|---|---|---|---|
| Excellent | Flow | Human | Anonymous | 05/19/2017 | ||||
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Summary
| Application | Flow Cytometry | |||||||||
| Sample Tested | A431 human epithelial carcinoma cell line | |||||||||
| Species | Human | |||||||||
| Excellent | Anonymous | 04/24/2017 |
| Flow Cytometry | |
|---|---|
 | Detection of ErbB2/Her2 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human ErbB2/Her2 Monoclonal Antibody (Catalog # MAB1129, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins. |
| Immunocytochemistry | |
 | ErbB2/Her2 in MDA‑MB‑231 Human Cell Line. ErbB2/Her2 was detected in immersion fixed MDA‑MB‑231 human breast cancer cell line using Mouse Anti-Human ErbB2/Her2 Monoclonal Antibody (Catalog # MAB1129) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
| Immunohistochemistry | |
 | ErbB2/Her2 in Human Breast Cancer Tissue. ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human ErbB2/Her2 Monoclonal Antibody (Catalog # MAB1129) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
| Immunohistochemistry | |
 | ErbB2/Her2 in Human Stomach. ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human ErbB2/Her2 Monoclonal Antibody (Catalog # MAB1129) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane/cytoplasm in glans and villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
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Related Product & Information
| Background | ErbB2/Her2 |
|---|---|
| background_content | Background: ErbB2/Her2 ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGF R), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation (1-10). |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
- CRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome
Authors: TS Klann, JB Black, M Chellappan, A Safi, L Song, IB Hilton, GE Crawford, TE Reddy, CA Gersbach
Nat. Biotechnol., 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Flow - Detection of Circulating Tumour Cells in Urothelial Cancers and Clinical Correlations: Comparison of Two Methods
Authors: E Fina, A Necchi, S Bottelli, C Reduzzi, S Pizzamigli, C Iacona, MG Daidone, P Verderio, V Cappellett
Dis. Markers, 2017;2017(0):3414910.
Species: Human
Sample Type: Whole Cells
Application: Flow - HER2 and EGFR overexpression support metastatic progression of prostate cancer to bone
Cancer Res., 2016;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Flow Ctyometry - Microbead arrays for the analysis of ErbB receptor tyrosine kinase activation and dimerization in breast cancer cells.
Authors: Khan IH, Zhao J, Ghosh P, Ziman M, Sweeney C, Kung HJ, Luciw PA
Assay Drug Dev Technol, 2010;8(1):27-36.
Species: Human
Sample Type: Cell Lysates
Application: IP
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製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
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