抗Osteopontin抗体(Anti-Osteopontin, Goat-Poly, Biotin antibody)
掲載日情報:2021/01/28 現在Webページ番号:29126
Osteopontinに対する抗体(Anti-Osteopontin, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 :2024年05月18日 00時00分現在]
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Anti-Osteopontin, Goat-Poly, Biotin <Anti-OPN> |
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[在庫・価格 :2024年05月18日 00時00分現在]
Anti-Osteopontin, Goat-Poly, Biotin <Anti-OPN>
文献数: 4
- 商品コード:BAF1433
- メーカー:RSD
- 包装:50μg
- 価格:¥119,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
マッチドペア:Human Osteopontin/OPN サンドイッチELISAの検出用抗体として利用可能,補足用抗体として#MAB14332-500,スタンダードとして#1433-OP-050を用いる。 別名:BNSP Genbank No: 6696 |
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保存条件 | -20℃ | 法規備考 | |
抗原種 | Human | 免疫動物 | Goat |
交差性 | Human | 適用 | ELISA,IC,Western Blot |
標識 | Biotin | 性状 | Antigen Affinity Purified |
吸収処理 | クラス | IgG | |
クロナリティ | Polyclonal | フォーマット | |
掲載カタログ |
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製品記事 | |||
関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
Species Reactivity | Human |
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Label | Biotin |
Immunogen | Human milk-derived Osteopontin/OPN |
Source | Polyclonal Goat IgG |
Purification | Antigen Affinity-purified |
Specificity | Detects human Osteopontin/OPN in ELISAs and Western blots. In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant mouse (rm) Osteopontin and bovine Osteopontin is observed. |
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Applications and Data
Recommended Concentration | Sample | |
Western Blot | 0.1 µg/mL | Recombinant Human Osteopontin/OPN (Catalog # 1433-OP) |
Immunocytochemistry | 5-15 µg/mL | Immersion fixed U2OS human osteosarcoma cell line |
Human Osteopontin/OPN Sandwich Immunoassay | Reagent | |
ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human Osteopontin/OPN Antibody (Catalog #MAB14332 ) |
ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human Osteopontin/OPN Biotinylated Antibody (Catalog #BAF1433 ) |
ELISA Standard | Recombinant Human Osteopontin (OPN) Protein (Catalog #1433-OP ) | |
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
Background: Osteopontin/OPN | Osteopontin (OPN, previously also referred to as transformation-associated secreted phosphoprotein, bone sialoprotein I, 2ar, 2B7, early T lymphocyte activation 1 protein, minopotin, calcium oxalate crystal growth inhibitor protein), is a secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein originally isolated from bone matrix (1). Subsequently, OPN has been found in kidney, placenta, blood vessels and various tumor tissues. Many cell types (including macrophages, osteoclasts, activated T-cells, fibroblasts, epithelial cells, vascular smooth muscle cells, and natural killer cells) can express OPN in response to activation by cytokines, growth factors or inflammatory mediators. Elevated expression of OPN has also been associated with numerous pathobiological conditions such as atherosclerotic plaques, renal tubulointerstitial fibrosis, granuloma formations in tuberculosis and silicosis, neointimal formation associated with balloon catheterization, metastasizing tumors, and cerebral ischemia. Human OPN cDNA encodes a 314 amino acid (aa) residue precursor protein with a 16 aa residue predicted signal peptide that is cleaved to yield a 298 aa residue mature protein with an integrin binding sequence (RGD), and N- and O-glycosylation sites. By alternative splicing, at least three human OPN isoforms exist. OPN has been shown to bind to different cell types through RGD-mediated interaction with the integrins alpha v beta 1, alpha v beta 3, alpha v beta 5, and non-RGD-mediated interaction with CD44 and the integrins alpha 8 beta 1 or alpha 9 beta 1. OPN exists both as a component of extracellular matrix and as a soluble molecule. Functionally, OPN is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. OPN has also been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Human, mouse, rat, pig and bovine OPN share from approximately 40% - 80% amino acid sequence identity. Osteopontin is a substrate for proteolytic cleavage by thrombin, enterokinase, MMP-3 and MMP-7. The functions of OPN in a variety of cell types were shown to be modified as a result of proteolytic cleavage (2, 3). |
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References
Ann. N.Y. Acad. Sci. (1995) 760, Apr. 21. | |
Senger, D.R. et al. (1996) Biochim. Biophys. Acta. 1314:13. | |
Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261. | |
Long Name: | Secreted Phosphoprotein 1 [BNSP] |
Entrez Gene IDs: | 6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine) |
Alternate Names: | BNSP; Bone sialoprotein 1; Eta-1; MGC110940; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1); secreted phosphoprotein-1 (osteopontin, bone sialoprotein); Spp1; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; uropontin |
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Related Research Areas
Adhesion Molecules in Angiogenesis | ||
Adhesion Molecules on VSMC | ||
Adipose-derived Stem Cell Markers | ||
Cancer Biomarkers | ||
Extracellular Matrix Molecules | ||
Gametes and Fertilization | ||
HIF Transcription Factors | ||
Integrins and Associated Molecules | ||
Molecules Secreted by VSMC | ||
Osteoblast Markers | ||
Osteogenesis Markers | ||
Renal Markers | ||
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Related Product & Information
Background | Osteopontin/OPN |
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background_content | Background: Osteopontin/OPN Osteopontin (OPN, previously also referred to as transformation-associated secreted phosphoprotein, bone sialoprotein I, 2ar, 2B7, early T lymphocyte activation 1 protein, minopotin, calcium oxalate crystal growth inhibitor protein), is a secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein originally isolated from bone matrix (1). Subsequently, OPN has been found in kidney, placenta, blood vessels and various tumor tissues. Many cell types (including macrophages, osteoclasts, activated T-cells, fibroblasts, epithelial cells, vascular smooth muscle cells, and natural killer cells) can express OPN in response to activation by cytokines, growth factors or inflammatory mediators. Elevated expression of OPN has also been associated with numerous pathobiological conditions such as atherosclerotic plaques, renal tubulointerstitial fibrosis, granuloma formations in tuberculosis and silicosis, neointimal formation associated with balloon catheterization, metastasizing tumors, and cerebral ischemia. Human OPN cDNA encodes a 314 amino acid (aa) residue precursor protein with a 16 aa residue predicted signal peptide that is cleaved to yield a 298 aa residue mature protein with an integrin binding sequence (RGD), and N- and O-glycosylation sites. By alternative splicing, at least three human OPN isoforms exist. OPN has been shown to bind to different cell types through RGD-mediated interaction with the integrins alpha v beta 1, alpha v beta 3, alpha v beta 5, and non-RGD-mediated interaction with CD44 and the integrins alpha 8 beta 1 or alpha 9 beta 1. OPN exists both as a component of extracellular matrix and as a soluble molecule. Functionally, OPN is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. OPN has also been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Human, mouse, rat, pig and bovine OPN share from approximately 40% - 80% amino acid sequence identity. Osteopontin is a substrate for proteolytic cleavage by thrombin, enterokinase, MMP-3 and MMP-7. The functions of OPN in a variety of cell types were shown to be modified as a result of proteolytic cleavage (2, 3). |
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Citations
- Gene expression signatures for tumor progression, tumor subtype, and tumor thickness in laser-microdissected melanoma tissues.
Authors: Jaeger J, Koczan D, Thiesen HJ, Ibrahim SM, Gross G, Spang R, Kunz M
Clin. Cancer Res., 2007;13(3):806-15.
Species: Human
Sample Type: Cell Lysates
Application: WB
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