抗IL-23 Receptor抗体(Anti-IL-23 Receptor, Human, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Biotin
Immunogen	Mouse myeloma cell line NS0-derived recombinant human IL-23 RGly24-Ile353Accession # Q5VWK5
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human IL-23 R in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse IL-23 R is observed.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human IL‑23 R Fc Chimera (Catalog # 1400-IR)

目次に戻る

Related Product & Information

Background

IL-23 R

background_content

Background: IL-23 R Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Human IL-23 R cDNA encodes a 629 amino acid (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 330 aa residue extracellular domain, a 23 aa residue transmembrane domain, and a 253 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential STAT-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% aa sequence identity. Based on quantitative real-time PCR, human IL-23 R mRNA is expressed in a human Th1 and Th0 clone as well as several NK cell lines and clones. Low but detectable levels of IL-23 R mRNA is also expressed in EBV-transformed B cells and activated PBMC. IL-23 initiates a signal transduction cascade similar to that of IL-12, and involves Jak2, Tyk2, STAT1, STAT3, STAT4, and STAT5. IL-23 has biological activities that are similar to, but distinct from, IL-12.

目次に戻る

Citations

1. The human IL-23 receptor rs11209026 A allele promotes the expression of a soluble IL-23R-encoding mRNA species.
   Authors: Yu R, Brazaitis J, Gallagher G
   J Immunol, 2015;194(3):1062-8.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA detection


2. The IL23R R381Q Gene Variant Protects against Immune-Mediated Diseases by Impairing IL-23-Induced Th17 Effector Response in Humans.
   Authors: Di Meglio P, Di Cesare A, Laggner U
   PLoS ONE, 2011;6(2):e17160.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Regulating human Th17 cells via differential expression of IL-1 receptor.
   Authors: Lee WW, Kang SW, Choi J
   Blood, 2010;115(3):530-40.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. A naturally occurring, soluble antagonist of human IL-23 inhibits the development and in vitro function of human Th17 cells.
   Authors: Yu RY, Gallagher G
   J. Immunol., 2010;185(12):7302-8.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


5. Prostaglandin E2 regulates Th17 cell differentiation and function through cyclic AMP and EP2/EP4 receptor signaling.
   Authors: Boniface K, Bak-Jensen KS, Li Y, Blumenschein WM, McGeachy MJ, McClanahan TK, McKenzie BS, Kastelein RA, Cua DJ, de Waal Malefyt R
   J. Exp. Med., 2009;206(3):535-48.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. IL-23 and IL-12 responses in activated human T cells retrovirally transduced with IL-23 receptor variants.
   Authors: de Paus RA, van de Wetering D, Van Dissel JT
   Mol. Immunol., 2008;45(15):3889-95.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

目次に戻る