抗E-Selectin抗体(Anti-E-Selectin, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Chinese hamster ovary cell line CHO-derived recombinant human E-Selectin/CD62E
Source	Polyclonal Goat Serum
Purification	N/A
Specificity	This antibody has been screened using CHO cells transfected with cDNAs for E-Selectin, P-Selectin, L-Selectin, ICAM-1 and VCAM‑1. This antibody has shown to be only reactive with E-Selectin.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1:1000 dilution	Recombinant Human E-Selectin/CD62E Fc Chimera (Catalog # 724-ES)
Immunohistochemistry	1:100 dilution	See below

Immunohistochemistry
	E‑Selectin/CD62E in Human Kidney Cancer Tissue. E‑Selectin/CD62E was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human E‑Selectin/CD62E Polyclonal Antibody (Catalog # BBA18) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
	E-Selectin/CD62E in LPS treated Human KidneyTissue. E-Selectin/CD62E was detected in acetone fixed cryosections of human kidney tissue using Goat Anti-Human E-Selectin/CD62E Polyclonal Antibody (Catalog # BBA18) overnight at 4 ° C. Tissues were stained with rabbit anti-goat secondary antibody and HRP polymer-conjugated anti-rabbit IgG followed by AEC+Substrate Chromogen (red) followed by counterstaining with hematoxylin (blue). Before incubation with the primary antibody, tissues were treated ex vivo with LPS and also treated to block endogenous peroxidase activity. The lower panel shows an absence of labeling when primary antibody is omitted. Experiments were carried out and images were provided by Dr. Grietje Molema, University of Groningen, The Netherlands.

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Related Product & Information

Entrez Gene IDs	6401 (Human); 20339 (Mouse); 25544 (Rat)
Background	E-Selectin/CD62E
background_content	Background: E-Selectin/CD62E E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E-Selectin on healthy and diseased tissue. The human and mouseE‑Selectin proteins share 81% amino acid similarity. E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.

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Citations

1. Outer membrane vesicles derived from Escherichia coli up-regulate expression of endothelial cell adhesion molecules in vitro and in vivo.
   Authors: Kim J, Yoon Y, Lee J, Choi E, Yi N, Park K, Park J, Lotvall J, Kim Y, Gho Y
   PLoS ONE, 2013;8(3):e59276.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
   Authors: Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J
   J Biol Chem, 2012;287(32):26478-94.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Endothelial adhesion molecule expression is unaltered in the peripheral nerve from patients with AIDS and distal sensory polyneuropathy.
   Authors: Fenzi F, Rossi F, Rava M, Cavallaro T, Ferrari S, Rizzuto N
   J. Neuroimmunol., 2006;178(1):111-6.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


4. Trafficking pathways and characterization of CD4 and CD8 cells recruited to the skin of humans experimentally infected with Haemophilus ducreyi.
   Authors: Humphreys TL, Baldridge LA, Billings SD, Campbell JJ, Spinola SM
   Infect. Immun., 2005;73(7):3896-902.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


5. TNFalpha increases the inflammatory response to vascular balloon injury without accelerating neointimal formation.
   Authors: Miller AM, McPhaden AR, Preston A, Wadsworth RM, Wainwright CL
   Atherosclerosis, 2005;179(1):51-9.
   Species: Rabbit
   Sample Type: Whole Tissue
   Application: Neut


6. E-selectin and ICAM-1 are incorporated into detergent-insoluble membrane domains following clustering in endothelial cells.
   Authors: Tilghman RW, Hoover RL
   FEBS Lett., 2002;525(1):83-7.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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