抗E-Selectin抗体(Anti-E-Selectin, Human, Mouse-Mono) antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• Related Research Areas
• Summary
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Activated HUVEC human umbilical vein endothelial cells
Source	Monoclonal Mouse IgG1 Clone # BBIG-E4
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects E‑Selectin/CD62E in ELISAs and Western blots. In immunofluorescence experiments, does not stain COS cells transfected with cDNA for human ICAM-1, PECAM‑1, L-Selectin, P-Selectin, or VCAM-1.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human E-Selectin/CD62E Fc Chimera (Catalog # 724-ES) under non-reducing conditions only
Immunohistochemistry	8-25 µg/mL	See below
Immunoprecipitation	Pigott, R. et al. (1991) J. Immunol. 147:130.
Immunocytochemistry	8-25 µg/mL	See below
Human E-Selectin/CD62E Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-10 µg/mL	Human E‑Selectin/CD62E Antibody (Catalog #BBA16 )
ELISA Detection (Matched Antibody Pair)	0.5-2.0 µg/mL	Human E‑Selectin/CD62E Biotinylated Antibody (Catalog #BBA8 )
ELISA Standard		Recombinant Human E-Selectin/CD62E Protein, CF (Catalog #ADP1 )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Immunocytochemistry	click image to view larger	E-Selectin/CD62E in HUVEC Human Cells. E-Selectin/CD62E was detected in immersion fixed HUVEC human umbilical vein endothelial cells activated with TNF‑ alpha (Catalog # 210-TA-010) using Mouse Anti-Human E‑Selectin/ CD62E Monoclonal Antibody (Catalog # BBA16) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry	click image to view larger	E‑Selectin/CD62E in Human Brain. E‑Selectin/CD62E was detected in immersion fixed paraffin-embedded sections of human brain (occipital cortex) using 10 µg/mL Mouse Anti-Human E‑Selectin/CD62E Monoclonal Antibody (Catalog # BBA16) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution	Sterile PBS to a final concentration of 0.5 mg/mL.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: E-Selectin/CD62E	E-Selectin, also known as Endothelial Leukocyte Adhesion Molecule 1 (ELAM-1),and Leukocyte-Endothelial Cell Adhesion Molecule 2 (LECAM-2), has beendesignated CD62E. It is a cell-surface glycoprotein expressed byactivated endothelium and it mediates the adhesion of blood neutrophils.
Entrez Gene IDs:	6401 (Human); 20339 (Mouse); 25544 (Rat)
Alternate Names:	CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E

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Related Research Areas

Show

Adhesion Molecules in Angiogenesis
Adhesion Molecules on Endothelial Cells
Endothelial Cell Differentiation Markers
Endothelial Cell Markers
Endothelial Progenitor and Endothelial Cell Markers
Lectins
Selectins
Optimizing human Treg immunotherapy by Treg subset selection and E-selectin ligand expression Authors: C Donnelly, B Dykstra, N Mondal, J Huang, BJ Kaskow, R Griffin, R Sackstein, C Baecher-Al Sci Rep, 2018;8(1):420. Species: Human Sample Type: Cell Lysates Application: WB
Images	Ratings	Applications	Species	Reviewed By	Date	Details
	Average		ICC/IF	Human	Anonymous	04/26/2017

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Summary

Application	Immunocytochemistry/Immunofluorescence
Sample Tested	HUVEC human umbilical vein endothelial cells
Species	Human
Other Experimental Details	HUVECs were stimulated with LPS for activation

Immunocytochemistry
	E-Selectin/CD62E in HUVEC Human Cells. E-Selectin/CD62E was detected in immersion fixed HUVEC human umbilical vein endothelial cells activated with TNF‑ alpha (Catalog # 210-TA-010) using Mouse Anti-Human E‑Selectin/CD62E Monoclonal Antibody (Catalog # BBA16) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
	E‑Selectin/CD62E in Human Brain. E‑Selectin/CD62E was detected in immersion fixed paraffin-embedded sections of human brain (occipital cortex) using 10 µg/mL Mouse Anti-Human E‑Selectin/CD62E Monoclonal Antibody (Catalog # BBA16) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Entrez Gene IDs	6401 (Human); 20339 (Mouse); 25544 (Rat)
Background	E-Selectin/CD62E
background_content	Background: E-Selectin/CD62E E-Selectin, also known as Endothelial Leukocyte Adhesion Molecule 1 (ELAM-1), and Leukocyte-Endothelial Cell Adhesion Molecule 2 (LECAM-2), has been designated CD62E. It is a cell-surface glycoprotein expressed by activated endothelium and it mediates the adhesion of blood neutrophils.

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Citations

1. Optimizing human Treg immunotherapy by Treg subset selection and E-selectin ligand expression
   Authors: C Donnelly, B Dykstra, N Mondal, J Huang, BJ Kaskow, R Griffin, R Sackstein, C Baecher-Al
   Sci Rep, 2018;8(1):420.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. A modular platform for targeted RNAi therapeutics
   Authors: R Kedmi, N Veiga, S Ramishetti, M Goldsmith, D Rosenblum, N Dammes, I Hazan-Hale, L Nahary, S Leviatan-B, M Harlev, M Behlke, I Benhar, J Lieberman, D Peer
   Nat Nanotechnol, 2018;0(0):.
   


3. Heme oxygenase-1 directly binds STAT3 to control the generation of pathogenic Th17 cells during neutrophilic airway inflammation
   Authors: X Lin, J Lv, J Lv, C Di, Y Zhang, T Zhou, J Liu, Z Xia
   Allergy, 2017;0(0):.
   


4. Anti-CD20 monoclonal antibody combined with adenovirus vector-mediated IL-10 regulates spleen CD4+/CD8+ T�cells and T-bet/GATA-3 expression in NOD mice
   Authors: A Tang, C Li, Z Chen, T Li
   Mol Med Rep, 2017;0(0):.
   


5. The Abl-1 kinase is dispensable for NK cell inhibitory signaling and is not involved in murine NK cell education
   Authors: S Ganesan, TL Thanh, N Kadri, BJ Chambers, S Meinke, P Brodin, E Vivier, DM Wetzel, AJ Koleske, P Höglund
   Scand. J. Immunol., 2017;0(0):.
   


6. Purinergic receptor transactivation by the ?2-adrenergic receptor increases intracellular Ca(2+) in non-excitable cells
   Authors: W Stallaert, ET van der We, AM Schonegge, B Plouffe, M Hogue, V Lukashova, A Inoue, S Ishida, J Aoki, C Le Gouill, M Bouvier
   Mol. Pharmacol, 2017;0(0):.
   


7. Localized delivery of curcumin from injectable gelatin/Thai silk fibroin microspheres for anti-inflammatory treatment of osteoarthritis in a rat model
   Authors: J Ratanavara, K Soontornvi, S Shuangshot, S Shuangshot, S Damrongsak
   Inflammopharmacology, 2017;0(0):.
   


8. Hepatic inflammation-fibrosis-cancer axis in the rat hepatocellular carcinoma induced by diethylnitrosamine
   Authors: YF Ding, ZH Wu, YJ Wei, L Shu, YR Peng
   J. Cancer Res. Clin. Oncol, 2017;0(0):.
   


9. AGER gene polymorphisms and soluble receptor for advanced glycation end product in patients with idiopathic pulmonary fibrosis
   Authors: K Yamaguchi, H Iwamoto, Y Horimasu, S Ohshimo, K Fujitaka, H Hamada, W Mazur, N Kohno, N Hattori
   Respirology, 2017;0(0):.
   


10. A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer
    Authors: BV Howley, LA Link, S Grelet, M El-Sabban, PH Howe
    Oncogene, 2017;0(0):.
    

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