抗CD44抗体(Anti-CD44, Hemopoietic, Human, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Recombinant human CD44v3-10 (includes the invariant N-terminal exons and CD44v3-10 exons)
Source	Monoclonal Mouse IgG2A Clone # 2C5
Purification	Protein A or G purified from ascites
Specificity	Detects human CD44s on a panel of CD44 transfected COS cells by flow cytometry (Fox, S.B. et al. (1994) Cancer Res. 54:4539). This antibody recognizes an epitope in the invariant N-terminal region of all CD44 protein isoforms.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below
Simple Western	20 µg/mL	See below
Flow Cytometry	2.5 µg/106 cells	Human whole blood monocytes
Immunohistochemistry	8-25 µg/mL	See below
Immunoprecipitation	Fox, S.B. et al. (1994) Cancer Res. 54:4539.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Western Blot
	Detection of Human CD44 by Western Blot. Western blot shows lysates of PC‑3 human prostate cancer cell line and human tonsil tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD44 s Pan Specific Monoclonal Antibody (Catalog # BBA10) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD44 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	CD44 in Human Lymphoma. CD44 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Mouse Anti-Human CD44 s Pan Specific Monoclonal Antibody (Catalog # BBA10) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western
	Detection of Human CD44 by Simple WesternTM. Simple Western lane view shows lysates of human tonsil tissue, loaded at 0.2 mg/mL. A specific band was detected for CD44 at approximately 156 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human CD44 s Pan Specific Monoclonal Antibody (Catalog # BBA10). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

目次に戻る

Related Product & Information

Background

CD44

background_content

Background: CD44 CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration (1-3). Human CD44 has a 20 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 325-530 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. Within the stem, ten variably spliced exons (v1-10, exons 6-15) produce multiple protein isoforms (1‑3). The standard or hematopoietic form, CD44s, does not include the variable segments (1‑3). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (1, 3). With variable N- and O-glycosylation and splicing within the stalk, CD44 can range from 80-200 kDa (1). Within the N‑terminal invariant portion of the ECD (aa 21-220), human CD44 shares 76%, 76%, 86%, 83%, and 79% identity with corresponding mouse, rat, equine, canine, and bovine CD44, respectively. The many reported functions of CD44 fall within three categories (1). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a “platform” for growth factors and metalloproteinases. Second, CD44 can function as a co-receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form (4) or may be cleaved at multiple sites by either membrane-type matrix metalloproteinases, or ADAM proteases to produce soluble ectodomains (5, 6). The cellular portion may then undergo gamma secretase-dependent intramembrane cleavage to form an A beta -like transmembrane portion and a cytoplasmic signaling portion that affects gene expression (7, 8). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 5).

目次に戻る

Citations

1. Optimizing human Treg immunotherapy by Treg subset selection and E-selectin ligand expression
   Authors: C Donnelly, B Dykstra, N Mondal, J Huang, BJ Kaskow, R Griffin, R Sackstein, C Baecher-Al
   Sci Rep, 2018;8(1):420.
   Species: Human
   Sample Type: Cell Lysates
   Application: IP


2. Akt signaling is sustained by a CD44 splice isoform-mediated positive feedback loop
   Authors: S Liu, C Cheng
   Cancer Res., 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Cell-Specific Variation in E-Selectin Ligand Expression among Human Peripheral Blood Mononuclear Cells: Implications for Immunosurveillance and Pathobiology
   Authors: M Silva, RK Fung, CB Donnelly, PA Videira, R Sackstein
   J. Immunol, 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: IP


4. Inhibiting the SUMO Pathway Represses the Cancer Stem Cell Population in Breast and Colorectal Carcinomas
   Stem Cell Reports, 2016;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Glycoengineering of E-selectin ligands by intracellular versus extracellular fucosylation differentially affects osteotropism of human mesenchymal stem cells
   Authors: Brad Dykstra
   Stem Cells, 2016;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: IP


6. Quantitative Characterization of E-selectin Interaction with Native CD44 and P-selectin Glycoprotein Ligand-1 (PSGL-1) Using a Real Time Immunoprecipitation-based Binding Assay.
   Authors: AbuSamra D, Al-Kilani A, Hamdan S, Sakashita K, Gadhoum S, Merzaban J
   J Biol Chem, 2015;290(35):21213-30.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


7. Coexpression of EpCAM, CD44 variant isoforms and claudin-7 in anaplastic thyroid carcinoma.
   Authors: Okada, Toshihir, Nakamura, Teruo, Watanabe, Takayuki, Onoda, Naoyoshi, Ashida, Atsuko, Okuyama, Ryuhei, Ito, Ken-ichi
   PLoS ONE, 2014;9(4):e94487.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


8. MIF inhibits monocytic movement through a non-canonical receptor and disruption of temporal Rho GTPase activities in U-937 cells.
   Authors: DiCosmo-Ponticello C, Hoover D, Coffman F, Cohen S, Cohen M
   Cytokine, 2014;69(1):47-55.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


9. CD26 expression on T-anaplastic large cell lymphoma (ALCL) line Karpas 299 is associated with increased expression of versican and MT1-MMP and enhanced adhesion.
   Authors: Havre, Pamela A, Dang, Long H, Ohnuma, Kei, Iwata, Satoshi, Morimoto, Chikao, Dang, Nam H
   BMC Cancer, 2013;13(0):517.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


10. KCa3.1 and TRPM7 channels at the uropod regulate migration of activated human T cells.
    Authors: Kuras Z, Yun Y, Chimote A, Neumeier L, Conforti L
    PLoS ONE, 2012;7(8):e43859.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

目次に戻る