抗Lipocalin-2抗体(Anti-Lipocalin-2, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived Recombinant Human Lipocalin‑2/NGALGln21-Gly198Accession # Q6FGL5
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Lipocalin-2/NGAL in direct ELISAs and Western blots.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below
Simple Western	2 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of Capan‑1 human pancreatic adenocarcinoma cell line and human pancreas tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1757) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Lipocalin‑2/NGAL at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot
	Detection of Mouse and Rat Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of mouse and rat bone marrow. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1757) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Lipocalin‑2/NGAL at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	Lipocalin‑2/NGAL in Human Pancreatic Cancer Tissue. Lipocalin‑2/NGAL was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Goat Anti-Human/Mouse/Rat Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1757) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
	Lipocalin‑2/NGAL in Human Pancreatic Cancer Tissue. Lipocalin‑2/NGAL was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Goat Anti-Human/Mouse/Rat Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1757) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western
	Detection of Human Lipocalin‑2/NGAL by Simple WesternTM. Simple Western lane view shows lysates of Capan‑1 human pancreatic adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Lipocalin‑2/NGAL at approximately 34 kDa (as indicated) using 2 µg/mL of Goat Anti-Human/Mouse/Rat Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1757) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

目次に戻る

Related Product & Information

Long Name	Neutrophil Gelatinase-associated Lipocalin
Background	Lipocalin-2/NGAL
background_content	Background: Lipocalin-2/NGAL Members of Lipocalin family share a highly conserved fold with an eight-stranded antiparallel beta barrel, and act as a transporters, carrying small molecules to specific cells. Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules. It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation. Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis. Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (Kd = 0.41 nM). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM). Secretion of Lipocalin‑2 in immune cells increases by stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron. Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway.

目次に戻る

Citations

1. Cilostazol attenuates kainic acid-induced hippocampal cell death
   Authors: YS Park, Z Jin, EA Jeong, CO Yi, JY Lee, IS Park, GS Roh
   Korean J. Physiol. Pharmacol., 2018;22(1):63-70.
   Species: Mouse
   Sample Type: Tissue Lysates
   Application: WB


2. Unexpected kidney-restricted role for IL-17 receptor signaling in defense against systemic Candida albicans infection
   Authors: K Ramani, CV Jawale, AH Verma, BM Coleman, JK Kolls, PS Biswas
   JCI Insight, 2018;3(9):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-P


3. Metformin alleviates nickel-induced autophagy and apoptosis via inhibition of hexokinase-2, activating lipocalin-2, in human bronchial epithelial cells
   Authors: YT Kang, WC Hsu, CH Wu, IL Hsin, PR Wu, KT Yeh, JL Ko
   Oncotarget, 2017;8(62):105536-105552.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. The adipokine lipocalin-2 in the context of the osteoarthritic osteochondral junction
   Sci Rep, 2016;6(0):29243.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: WB


5. Expression of uterine lipocalin 2 and its receptor during early- to mid-pregnancy period in mares
   Authors: Shingo Haneda
   J. Reprod. Dev, 2016;0(0):.
   Species: Equine
   Sample Type: Luminal Fluid
   Application: WBy


6. Lipocalin2 promotes invasion, tumorigenicity and gemcitabine resistance in pancreatic ductal adenocarcinoma.
   Authors: Leung, Lisa, Radulovich, Nikolina, Zhu, Chang-Qi, Organ, Shawna, Bandarchi, Bizhan, Pintilie, Melania, To, Christin, Panchal, Devang, Tsao, Ming Sou
   PLoS ONE, 2012;7(10):e46677.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


7. Cellular settings mediating Src Substrate switching between focal adhesion kinase tyrosine 861 and CUB-domain-containing protein 1 (CDCP1) tyrosine 734.
   Authors: Wortmann A, He Y, Christensen ME, Linn M, Lumley JW, Pollock PM, Waterhouse NJ, Hooper JD
   J. Biol. Chem., 2011;286(49):42303-15.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


8. Urinary neutrophil gelatinase-associated lipocalin levels reflect damage to glomeruli, proximal tubules, and distal nephrons.
   Authors: Kuwabara T, Mori K, Mukoyama M, Kasahara M, Yokoi H, Saito Y, Yoshioka T, Ogawa Y, Imamaki H, Kusakabe T, Ebihara K, Omata M, Satoh N, Sugawara A, Barasch J, Nakao K
   Kidney Int., 2009;75(3):285-94.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen

目次に戻る