抗CD163抗体(Anti-CD163, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human CD163 (R&D Systems, Catalog # 1607-CD)Gly41-Ser1045Accession # CAA80543
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human CD163 in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below

Western Blot
	Detection of Human CD163 by Western Blot. Western blot shows lysates of human thymus tissue, human liver tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD163 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1607) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD163 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western
	Detection of Human CD163 by Simple WesternTM. Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for CD163 at approximately 179 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CD163 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1607) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

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Related Product & Information

Background

CD163

background_content

Background: CD163 CD163, previously called M130 or p155, is a 130-160 kDa type I transmembrane glycoprotein that belongs to group B of the cysteine-rich scavenger receptor family (1-3). It is essential for clearance of hemoglobin-haptoglobin (Hb-Hp) complexes in the liver, spleen and circulation (4). The human CD163 contains a 41 amino acid (aa) signal sequence, a 1009 aa extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 22 aa transmembrane segment, and a39‑84 aa cytoplasmic region (1). The third SRCR domain is crucial for calcium-dependent binding of hemoglobin/haptoglobin complexes (3). Three splice forms (isoforms 2, 3 and 4) vary within their intracellular regions (1, 5), while one isoform (# 4) also has a 34 aa insert between SRCR domains 5 and 6 within the ECD. While all are expressed, isoform 3 is the most abundant, being generally expressed on the cell surface and most active in endocytosis (5). An approximately 130 kDa soluble form of human CD163 (sCD163) is assumed to contain virtually all of the ECD, which shares 74%, 75%, 84%, 86%, 86%, and 87% aa identity with mouse, rat, bovine, equine, porcine and canine CD163 ECD, respectively (6, 7). It is released from the cell surface by proteolysis after oxidative stress or inflammatory stimuli, including bacterial endotoxins and activation of the Toll-like receptors TLR2 or TLR5 (7 - 10). Expression of CD163 is constitutive, and induced by glucocorticoids, IL-10, IL-6 or endotoxin on circulating monocytes, tissue macrophages, and at low levels on monocyte-derived dendritic cells (1, 2, 11, 12). In addition to clearing Hb-Hp complexes, CD163 is also a scavenger receptor for free Hb (if Hp is depleted) and TWEAK (TNF-like weak inducer of apoptosis), and can function as an erythroblast adhesion receptor (4, 13-15).

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Citations

1. Stabilin-1 is expressed in human breast cancer and supports tumor growth in mammary adenocarcinoma mouse model
   Oncotarget, 2016;7(21):31097-110.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


2. Simian hemorrhagic fever virus cell entry is dependent on CD163 and uses a clathrin-mediated endocytosis-like pathway.
   Authors: Cai Y, Postnikova E, Bernbaum J, Yu S, Mazur S, Deiuliis N, Radoshitzky S, Lackemeyer M, McCluskey A, Robinson P, Haucke V, Wahl-Jensen V, Bailey A, Lauck M, Friedrich T, O'Connor D, Goldberg T, Jahrling P, Kuhn J
   J Virol, 2015;89(1):844-56.
   Species: Primate - Chlorocebus aethiops (African Green Monkey)
   Sample Type: Whole Cells
   Application: Neut


3. CD163 and IgG codefend against cytotoxic hemoglobin via autocrine and paracrine mechanisms.
   Authors: Subramanian K, Du R, Tan N, Ho B, Ding J
   J Immunol, 2013;190(10):5267-78.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Interaction of the European genotype porcine reproductive and respiratory syndrome virus (PRRSV) with sialoadhesin (CD169/Siglec-1) inhibits alveolar macrophage phagocytosis.
   Authors: De Baere MI, Van Gorp H, Delputte PL
   Vet. Res., 2012;43(1):47.
   Species: Porcine
   Sample Type: Whole Cells
   Application: Neut


5. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction.
   Authors: Zizzo G, Hilliard B, Monestier M, Cohen P
   J Immunol, 2012;189(7):3508-20.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


6. Identification of the CD163 protein domains involved in infection of the porcine reproductive and respiratory syndrome virus.
   Authors: Van Gorp H, Van Breedam W, Van Doorsselaere J, Delputte PL, Nauwynck HJ
   J. Virol., 2010;84(6):3101-5.
   Species: Porcine
   Sample Type: Whole Cells
   Application: WB


7. Sialoadhesin and CD163 join forces during entry of the porcine reproductive and respiratory syndrome virus.
   Authors: Van Gorp H, Van Breedam W, Delputte PL, Nauwynck HJ
   J. Gen. Virol., 2008;89(0):2943-53.
   Species: Porcine
   Sample Type: Whole Cells
   Application: ICC


8. Availability of activated CD4+ T cells dictates the level of viremia in naturally SIV-infected sooty mangabeys.
   Authors: Klatt NR, Villinger F, Bostik P, Gordon SN, Pereira L, Engram JC, Mayne A, Dunham RM, Lawson B, Ratcliffe SJ, Sodora DL, Else J, Reimann K, Staprans SI, Haase AT, Estes JD, Silvestri G, Ansari AA
   J. Clin. Invest., 2008;118(6):2039-49.
   Species: Primate - Cercocebus torquatus (Sooty Mangabey)
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. CD163 expression confers susceptibility to porcine reproductive and respiratory syndrome viruses.
   Authors: Calvert JG, Slade DE, Shields SL, Jolie R, Mannan RM, Ankenbauer RG, Welch SK
   J. Virol., 2007;81(14):7371-9.
   Species: Primate - Macaca mulatta (Rhesus Macaque)
   Sample Type: Whole Cells
   Application: Neut

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