抗SOST抗体(Anti-SOST, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse SOST/SclerostinGln24-Tyr211Accession # NP_077769
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse SOST/Sclerostin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human SOST is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human and Mouse SOST/Sclerostin by Western Blot. Western blot shows lysates of human bone marrow and mouse bone marrow. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SOST/Sclerostin at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	SOST/Sclerostin in Mouse Embryo. SOST/Sclerostin was detected in immersion fixed frozen sections of mouse embryo (adrenal gland) using Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
	SOST/Sclerostin in Mouse Embryo. SOST/Sclerostin was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Background

SOST/Sclerostin

background_content

Background: SOST/Sclerostin SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. Cerberus/DAN family members are putative BMP antagonists, and include Dan, Cerberus, Gremlin, PRDC, and Caronte. While the overall sequence identity between members of the family is low, they have conserved spacing of six cysteine residues. Cerberus and Dan have an additional cysteine residue used for dimerization; however, SOST does not and is secreted as a monomer. SOST was originally identified as an important regulator of bone homeostasis. Positional cloning studies identified that mutations in the SOST gene can cause sclerosteosis and van Buchem disease, bone dysplasia disorders characterized by progressive skeletal overgrowth. Significant levels of SOST expression are detected in bone, cartilage, kidney, and liver. SOST is expressed by osteoclasts in developing bones of mouse embryos, including both intramembranously forming skull bones and endochondrally forming long bones. SOST plays a physiological role as a negative regulator of bone formation by repressing BMP-induced osteogenesis. SOST has been shown to have unique ligand specificity, binding BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. This seems to be the first example of a BMP antagonist being localized to osteoclasts, cells derived from the hematopoietic lineage, that function to degrade bone matrix. Human and mouse SOST share 88% amino acid identity (1-3).

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Citations

1. Transient peak-strain matching partially recovers the age-impaired mechanoadaptive cortical bone response
   Authors: B Javaheri, A Carriero, M Wood, R De Souza, PD Lee, S Shefelbine, AA Pitsillide
   Sci Rep, 2018;8(1):6636.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-P


2. Bone adaptation in response to treadmill exercise in young and adult mice
   Authors: JD Gardinier, N Rostami, L Juliano, C Zhang
   Bone Rep, 2018;8(0):29-37.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-P


3. Chronological immunolocalization of sclerostin and FGF23 in the mouse metaphyseal trabecular and cortical bone
   Authors: A Sakurai, T Hasegawa, A Kudo, Z Shen, T Nagai, M Abe, T Yoshida, H Hongo, T Yamamoto, T Yamamoto, K Oda, PHL Freitas, M Li, H Sano, N Amizuka
   Biomed. Res., 2017;38(4):257-267.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC paraffin embedded


4. Zoledronate promotes bone formation by blocking osteocyte-osteoblast communication during bone defect healing
   Authors: P Cui, H Liu, J Sun, N Amizuka, Q Sun, M Li
   Histol. Histopathol., 2017;0(0):11893.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


5. Gs? Controls Cortical Bone Quality by Regulating Osteoclast Differentiation via cAMP/PKA and ?-Catenin Pathways
   Authors: G Ramaswamy, H Kim, D Zhang, V Lounev, JY Wu, Y Choi, FS Kaplan, RJ Pignolo, EM Shore
   Sci Rep, 2017;7(0):45140.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


6. Sclerostin Promotes Bone Remodeling in the Process of Tooth Movement
   Authors: R Shu, D Bai, T Sheu, Y He, X Yang, C Xue, Y He, M Zhao, X Han
   PLoS ONE, 2017;12(1):e0167312.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


7. Suppression of Sclerostin Alleviates Radiation-Induced Bone Loss by Protecting Bone Forming Cells and Their Progenitors through Distinct Mechanisms
   J Bone Miner Res, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


8. Inflammatory bowel disease in a rodent model alters osteocyte protein levels controlling bone turnover
   Authors: S A Bloomfield
   J. Bone Miner. Res., 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


9. Short-term intermittent administration of parathyroid hormone facilitates osteogenesis by different mechanisms in cancellous and cortical bone
   Authors: K Ogura, T Iimura, Y Makino, A Sugie-Oya, A Takakura, R Takao-Kawa, T Ishizuya, K Moriyama, A Yamaguchi
   Bone Rep, 2016;5(0):7-14.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC


10. Distorted Patterns of Dentinogenesis and Eruption in Msx2 Null Mutants: Involvement of Sost/Sclerostin
    Am J Pathol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded

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