抗MCP-1抗体(Anti-MCP-1, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse CCL2/JE/MCP-1Gln24-Arg96Accession # Q5SVU3
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse CCL2/JE/MCP-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.01% cross-reactivity with recombinant human (rh) MCP‑1, rhMCP‑2, rhMCP-3, recombinant mouse (rm) MARC, rhMCP-4, rmMCP-5, rmMDC, rmMIP-1 alpha, rmMIP-1 beta, rmMIP-1 gamma,rmMIP‑2, rmMIP‑3 beta, rmRANTES, rmTARC, and rmTeck is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	See below
Mouse CCL2/JE/MCP-1 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	0.2-0.8 µg/mL	Mouse CCL2/JE/MCP‑1 Antibody (Catalog #AF-479-NA )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse CCL2/JE/MCP‑1 Biotinylated Antibody (Catalog #BAF479 )
ELISA Standard		Recombinant Mouse CCL2/JE/MCP-1 Protein (Catalog #479-JE )
Neutralization	Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 0.04 µg/mL Recombinant Mouse CCL2/JE/MCP‑1.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Western Blot	click image to view larger	Detection of Recombinant Mouse CCL2/JE/MCP‑1 by Western Blot. Western blot shows 25 ng of Recombinant Mouse CCL2/JE/MCP‑1 (Catalog # 479-JE), Recombinant Human CCL2/JE/MCP‑1 (Catalog # 279-MC), Recombinant Rat CCL2/JE/MCP‑1 (Catalog # 3144-JE), Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME), Recombinant Mouse CCL12/MCP‑5 (Catalog # 428-P5), and Recombinant Mouse CCL7/MCP‑3/MARC (Catalog # 456-MC). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL2/JE/ MCP‑1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Neutralization	click image to view larger	Chemotaxis Induced by CCL2/JE and Neutralization by Mouse CCL2/JE Antibody.Recombinant Mouse CCL2/JE (Catalog # 479-JE) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL2/JE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA). The ND50 is typically 0.5-2.5 µg/mL.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL2/JE/MCP-1	Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The murine CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in murine fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.
Entrez Gene IDs:	6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
Alternate Names:	C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF

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Related Research Areas

Alpha Granule Soluble Molecules

CC Chemokines and Receptors

Chemokines and Receptors

Chemokines and Receptors in Allergy and Asthma

Chemokines and Receptors in Angiogenesis

gamma delta T Cells

HIF Transcription Factors

Inflammatory Mediators

Macrophage Activation Markers

Molecules Secreted by VSMC

Neuroinflammation

Uptake of Amyloid beta by Macrophages

Hepatocyte nuclear receptor SHP suppresses inflammation and fibrosis in a mouse model of nonalcoholic steatohepatitis Authors: A Zou, N Magee, F Deng, S Lehn, C Zhong, Y Zhang J. Biol. Chem., 2018;0(0):. Species: Mouse Sample Type: Whole Cells Application: Neut

Western Blot
	Detection of Recombinant Mouse CCL2/JE/MCP‑1 by Western Blot. Western blot shows 25 ng of Recombinant Mouse CCL2/JE/MCP‑1 (Catalog # 479-JE), Recombinant Human CCL2/JE/MCP‑1 (Catalog # 279-MC), Recombinant Rat CCL2/JE/MCP‑1 (Catalog # 3144-JE), Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME), Recombinant Mouse CCL12/MCP‑5 (Catalog # 428-P5), and Recombinant Mouse CCL7/MCP‑3/MARC (Catalog # 456-MC). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL2/JE/MCP‑1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Neutralization
	Chemotaxis Induced by CCL2/JE and Neutralization by Mouse CCL2/JE Antibody. Recombinant Mouse CCL2/JE (Catalog # 479-JE) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL2/JE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA). The ND50 is typically 0.5-2.5 µg/mL.

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Related Product & Information

Entrez Gene IDs	6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
Background	CCL2/JE/MCP-1
background_content	Background: CCL2/JE/MCP-1 Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The murine CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in murine fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.

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Citations

1. Hepatocyte nuclear receptor SHP suppresses inflammation and fibrosis in a mouse model of nonalcoholic steatohepatitis
   Authors: A Zou, N Magee, F Deng, S Lehn, C Zhong, Y Zhang
   J. Biol. Chem., 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


2. The Chemokine CCL2 Mediates the Seizure-enhancing Effects of Systemic Inflammation
   Authors: C Cerri, S Genovesi, M Allegra, F Pistillo, U Püntener, A Guglielmot, VH Perry, Y Bozzi, M Caleo
   J Neurosci, 2016;36(13):3777-88.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


3. Vitamin D limits chemokine expression in adipocytes and macrophage migration in vitro and in male mice.
   Authors: Karkeni E, Marcotorchino J, Tourniaire F, Astier J, Peiretti F, Darmon P, Landrier J
   Endocrinology, 2015;156(5):1782-93.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


4. P2RX7 sensitizes Mac-1/ICAM-1-dependent leukocyte-endothelial adhesion and promotes neurovascular injury during septic encephalopathy.
   Authors: Wang H, Hong L, Huang J, Jiang Q, Tao R, Tan C, Lu N, Wang C, Ahmed M, Lu Y, Liu Z, Shi W, Lai E, Wilcox C, Han F
   Cell Res, 2015;25(6):674-90.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


5. GEC-derived SFRP5 inhibits Wnt5a-induced macrophage chemotaxis and activation.
   Authors: Zhao C, Bu X, Wang W, Ma T, Ma H
   PLoS ONE, 2014;9(1):e85058.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. IL-1beta mediates MCP-1 induction by Wnt5a in gastric cancer cells.
   Authors: Li S, Wang W, Zhang N, Ma T, Zhao C
   BMC Cancer, 2014;14(0):480.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. Persistent inflammation subverts thrombospondin-1-induced regulation of retinal angiogenesis and is driven by CCR2 ligation.
   Authors: Chen M, Copland DA, Zhao J, Liu J, Forrester JV, Dick AD, Xu H
   Am. J. Pathol., 2012;180(1):235-45.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


8. Response patterns of cytokines/chemokines in two murine strains after irradiation.
   Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
   Cytokine, 2012;58(2):169-77.
   Species: Mouse
   Sample Type: Plasma
   Application: Luminex Assay Development


9. Bovine lactoferrin counteracts Toll-like receptor mediated activation signals in antigen presenting cells.
   Authors: Puddu P, Latorre D, Carollo M, Catizone A, Ricci G, Valenti P, Gessani S
   PLoS ONE, 2011;6(7):e22504.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


10. Toll-like receptor 2 contributes to chemokine gene expression and macrophage infiltration in the dorsal root ganglia after peripheral nerve injury.
    Authors: Kim D, You B, Lim H, Lee SJ
    Mol Pain, 2011;7(0):74.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC

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