抗FABP5抗体(Anti-FABP5, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse FABP5/E‑FABPMet1-Gln135Accession # Q05816
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse FABP5/E‑FABP in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse FABP4 and recombinant human (rh) FABP3 is observed and less than 1% cross-reactivity with recombinant rat (rr) FABP2 and rrFABP1 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	Perfusion fixed frozen sections of mouse intestine, liver, skin, and thymus

Western Blot

Detection of Human, Mouse, and Rat FABP5/E‑FABP by Western Blot. Western blot shows lysates of human placenta tissue, mouse liver tissue, rat brain tissue, and rat heart tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat FABP5/E‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1476) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP5/E‑FABP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Related Product & Information

Long Name	Fatty Acid-Binding Protein 5
Background	FABP5/E-FABP
background_content	Background: FABP5/E-FABP FABP5, also known as epidermal fatty acid binding protein (E-FABP), is a 15 kDa member of a cytosolic fatty acid binding protein superfamily. It is associated with keratinocytes and adipocytes and is suggested to promote fatty acid availability to enzymes, protect cell structures from fatty acid attack, and target fatty acids to nuclear transcription factors. The amino acid sequence of human FABP5 is 80%, 81% and 92% identical to that of mouse, rat and bovine FABP5, respectively.

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Citations

1. Adipose Fatty Acid Binding Protein Promotes Saturated Fatty Acid-Induced Macrophage Cell Death through Enhancing Ceramide Production
   Authors: Yuwen Zhang
   J. Immunol, 2016;0(0):.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


2. BLIMP1 is required for postnatal epidermal homeostasis but does not define a sebaceous gland progenitor under steady-state conditions.
   Authors: Kretzschmar K, Cottle D, Donati G, Chiang M, Quist S, Gollnick H, Natsuga K, Lin K, Watt F
   Stem Cell Reports, 2014;3(4):620-33.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC


3. Fatty acid-binding protein E-FABP restricts tumor growth by promoting IFN-beta responses in tumor-associated macrophages.
   Authors: Zhang Y, Sun Y, Rao E, Yan F, Li Q, Zhang Y, Silverstein K, Liu S, Sauter E, Cleary M, Li B
   Cancer Res, 2014;74(11):2986-98.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


4. Fatty acid-binding protein 5 (FABP5) regulates cognitive function both by decreasing anandamide levels and by activating the nuclear receptor peroxisome proliferator-activated receptor beta/delta (PPARbeta/delta) in the brain.
   Authors: Yu S, Levi L, Casadesus G, Kunos G, Noy N
   J Biol Chem, 2014;289(18):12748-58.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


5. Retinoic acid induces neurogenesis by activating both retinoic acid receptors (RARs) and peroxisome proliferator-activated receptor beta/delta (PPARbeta/delta).
   Authors: Yu S, Levi L, Siegel R, Noy N
   J Biol Chem, 2012;287(50):42195-205.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


6. Proteomic analysis of proteins differentially expressed in uterine lymphocytes obtained from wild-type and NOD mice.
   Authors: Li C, Wang W, Wang H, Zhong Y, Di J, Lin Y
   J. Cell. Biochem., 2009;108(2):447-57.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


7. All-trans-retinoic acid represses obesity and insulin resistance by activating both peroxisome proliferation-activated receptor beta/delta and retinoic acid receptor.
   Authors: Berry DC, Noy N
   Mol. Cell. Biol., 2009;29(12):3286-96.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


8. Helper-dependent adenovirus-mediated short hairpin RNA expression in the liver activates the interferon response.
   Authors: Witting SR, Brown M, Saxena R, Nabinger S, Morral N
   J. Biol. Chem., 2007;283(4):2120-8.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB

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