抗6Ckine抗体(Anti-6Ckine, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse CCL21/6CkineSer24-Gly133Accession # P84444
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse CCL21/6Ckine in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross reactivity with recombinant rat CCL21/6Ckine is observed and less than 15% cross-reactivity with recombinant human CCL21/6Ckine is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse CCL21/6Ckine (Catalog # 457-6C)
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	2.5 µg/106 cells	See below
Neutralization	Measured by its ability to neutralize CCL21/6Ckine-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR7. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 50 ng/mL Recombinant Mouse CCL21/6Ckine.

Neutralization
	Chemotaxis Induced by CCL21/6Ckine and Neutralization by Mouse CCL21/6Ckine Antibody. Recombinant Mouse CCL21/6Ckine (Catalog # 457‑6C) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL21/6Ckine (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL21/6Ckine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF457). The ND50 is typically 0.5‑2.5 µg/mL.
Immunohistochemistry
	CCL21/6Ckine in Mouse Thymus. CCL21/6Ckine was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse CCL21/6Ckine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF457) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Intracellular Staining by Flow Cytometry
	Detection of CCL21/6Ckine in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Goat Anti-Mouse CCL21/6Ckine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF457, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).  To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

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Related Product & Information

Entrez Gene IDs	6366 (Human); 18829 (Mouse); 298006 (Rat)
Background	CCL21/6Ckine
background_content	Background: CCL21/6Ckine 6Ckine is a novel CC chemokine discovered independently by three groups from the EST database. 6Ckine, also named SLC (secondary lymphoid-tissue chemokine), CCL21 and Exodus-2, shows 21-33% identity to other CC chemokines. 6Ckine contains the four conserved cysteines characteristic of beta chemokines plus two additional cysteines in its unusually long carboxyl-terminal domain. Human 6Ckine cDNA encodes a 134 amino acid residue, highly basic, precursor protein with a 23 amino acid residue signal peptide that is cleaved to form the predicted 111 amino acid residue mature protein. Mouse 6Ckine cDNA encodes a 133 amino acid residue protein with a 23 residue signal peptide that is cleaved to generate the 110 residue mature protein. Human and mouse 6Ckine are highly conserved, exhibiting 86% amino acid sequence identity. 6Ckine is constitutively expressed at high levels in lymphoid tissues such as lymph nodes, spleen and appendix. In mouse, high levels of 6Ckine mRNA are also detected in the lung. The gene for human 6Ckine has been localized at human chromosome 9p13 rather than chromosome 17 where the genes of many human CC chemokines are clustered. The 6Ckine gene location is within a region of about 100 kb from the MIP-3 beta /ELC gene, another identified novel CC chemokine. Unlike most CC chemokines, 6Ckine is not chemotactic for monocytes. 6Ckine has also been reported to inhibit hemopoietic progenitor colony formation in a dose-dependent manner. 6Ckine acts via a class of as yet unidentified CC receptors on both T cells and B cells that are not shared by any other CC chemokines. Mature rat CCL21 shares 84% aa sequence identity with mouse CCL21.

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Citations

1. Expression of the Atypical Chemokine Receptor ACKR4 Identifies a Novel Population of Intestinal Submucosal Fibroblasts That Preferentially Expresses Endothelial Cell Regulators
   Authors: CA Thomson, SA van de Pav, M Stakenborg, E Labeeuw, G Matteoli, AM Mowat, RJB Nibbs
   J. Immunol., 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. Dendritic Cells Interpret Haptotactic Chemokine Gradients in a Manner Governed by Signal-to-Noise Ratio and Dependent on GRK6
   Authors: J Schwarz, V Bierbaum, K Vaahtomeri, R Hauschild, M Brown, I de Vries, A Leithner, A Reversat, J Merrin, T Tarrant, T Bollenbach, M Sixt
   Curr. Biol., 2017;0(0):.
   Species: Mouse
   Sample Type: Complex Sample Type
   Application: IHC


3. Role of SIRP? in Homeostatic Regulation of T Cells and Fibroblastic Reticular Cells in the Spleen
   Authors: D Respatika, Y Saito, K Washio, S Komori, T Kotani, H Okazawa, Y Murata, T Matozaki
   Kobe J Med Sci, 2017;63(1):E22-E29.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-F


4. Increased migration of antigen presenting cells to newly-formed lymphatic vessels in transplanted kidneys by glycol-split heparin
   Authors: DT Talsma, K Katta, M Boersema, S Adepu, A Naggi, G Torri, C Stegeman, G Navis, H van Goor, JL Hillebrand, S Yazdani, J van den Bo
   PLoS ONE, 2017;12(6):e0180206.
   Species: Human
   Sample Type: Recombinant Proteins
   Application: Functional Assay


5. Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration
   Authors: C Colin Brinkman
   Nat Commun, 2016;7(0):12021.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: Functional Assay


6. Structural and functional features of central nervous system lymphatic vessels.
   Authors: Louveau A, Smirnov I, Keyes T, Eccles J, Rouhani S, Peske J, Derecki N, Castle D, Mandell J, Lee K, Harris T, Kipnis J
   Nature, 2015;523(7560):337-41.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Not Specified


7. Collecting lymphatic vessel permeability facilitates adipose tissue inflammation and distribution of antigen to lymph node-homing adipose tissue dendritic cells.
   Authors: Kuan E, Ivanov S, Bridenbaugh E, Victora G, Wang W, Childs E, Platt A, Jakubzick C, Mason R, Gashev A, Nussenzweig M, Swartz M, Dustin M, Zawieja D, Randolph G
   J Immunol, 2015;194(11):5200-10.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Not Specified


8. TWEAK promotes peritoneal inflammation.
   Authors: Sanz A, Aroeira L, Bellon T, del Peso G, Jimenez-Heffernan J, Santamaria B, Sanchez-Nino M, Blanco-Colio L, Lopez-Cabrera M, Ruiz-Ortega M, Egido J, Selgas R, Ortiz A
   PLoS ONE, 2014;9(3):e90399.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. Increased expression of the homeostatic chemokines CCL19 and CCL21 in clinical and experimental Rickettsia conorii infection.
   Authors: Astrup E, Ranheim T, Damas J, Davi G, Santilli F, Jensenius M, Vitale G, Aukrust P, Olano J, Otterdal K
   BMC Infect Dis, 2014;14(0):70.
   


10. The Schlemm's canal is a VEGF-C/VEGFR-3-responsive lymphatic-like vessel.
    Authors: Aspelund A, Tammela T, Antila S, Nurmi H, Leppanen V, Zarkada G, Stanczuk L, Francois M, Makinen T, Saharinen P, Immonen I, Alitalo K
    J Clin Invest, 2014;124(9):3975-86.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh

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