抗MIP-2抗体(Anti-MIP-2, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse CXCL2/MIP-2Ala28-Asn100Accession # P10889
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse CXCL2/MIP-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) GRO alpha, rhGRO beta, rhGRO gamma, recombinant rat (rr) CINC-1, rrCINC-2 alpha, rrCINC-2 beta, and rrCINC-3 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse CXCL2/MIP‑2 (Catalog # 452-M2)
Immunohistochemistry	5-15 µg/mL	Perfusion fixed frozen sections of mouse thymus
Immunocytochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize CXCL2/MIP-2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.015-0.075 µg/mL in the presence of 2 ng/mL Recombinant Mouse CXCL2/MIP-2.

Neutralization
	Chemotaxis Induced by CXCL2/MIP-2 and Neutral­ization by Mouse CXCL2/MIP-2 Antibody. Recombinant Mouse CXCL2/MIP-2 (Catalog # 452‑M2) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL2/MIP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-452-NA). The ND50 is typically 0.015‑0.075 µg/mL.
Immunocytochemistry
	CXCL2/MIP‑2 in Mouse Splenocytes. CXCL2/MIP‑2 was detected in immersion fixed mouse splenocytes stimulated with LPS and monensin using Goat Anti-Mouse CXCL2/MIP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-452-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Entrez Gene IDs	2920 (Human); 20310 (Mouse); 114105 (Rat)
Background	CXCL2/GRO beta/MIP-2/CINC-3
background_content	Background: CXCL2/GRO beta/MIP-2/CINC-3 Macrophage Inflammatory Protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. Based on its protein and DNA sequences, MIP-2 is a member of the alpha (C-X-C) subfamily of chemokines. MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature MIP-2. The protein sequence of murine MIP-2 shows approximately 63% identity to that of murine KC, another murine alpha chemokine whose expression is induced by PDGF. In addition, the protein sequence of MIP-2 is also 60% identical to human GRO beta and GRO gamma. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs. Similarly to other alpha chemokines, murine MIP-2 is a potent neutrophil attractant and activator. MIP-2 and KC can bind the murine interleukin 8 type B receptor homologue with high affinity. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases.

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Citations

1. Novel adherent CD11b+Gr-1+tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment
   Authors: T Tsubaki, T Kadonosono, S Sakurai, T Shiozawa, T Goto, S Sakai, T Kuchimaru, T Sakamoto, H Watanabe, G Kondoh, S Kizaka-Kon
   Oncotarget, 2018;9(13):11209-11226.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Mycobacterium tuberculosis-triggered Hippo pathway orchestrates CXCL1/2 expression to modulate host immune responses
   Sci Rep, 2016;6(0):37695.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


3. TIARP attenuates autoantibody-mediated arthritis via the suppression of neutrophil migration by reducing CXCL2/CXCR2 and IL-6 expression
   Sci Rep, 2016;6(0):38684.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Bioassay


4. Mycobacterium tuberculosis-triggered Hippo pathway orchestrates CXCL1/2 expression to modulate host immune responses
   Sci Rep, 2016;6(0):37695.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Bioassay


5. Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancy.
   Authors: Mizugishi K, Inoue T, Hatayama H, Bielawski J, Pierce J, Sato Y, Takaori-Kondo A, Konishi I, Yamashita K
   J Biol Chem, 2015;290(4):2053-68.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


6. The HMGB1/RAGE axis triggers neutrophil-mediated injury amplification following necrosis.
   Authors: Huebener, Peter, Pradere, Jean-Phi, Hernandez, Celine, Gwak, Geum-You, Caviglia, Jorge Ma, Mu, Xueru, Loike, John D, Jenkins, Rosalind, Antoine, Daniel J, Schwabe, Robert F
   J Clin Invest, 2015;125(2):539-50.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


7. Neutrophil dependence of vascular remodeling after Mycoplasma infection of mouse airways.
   Authors: Baluk P, Phillips K, Yao L, Adams A, Nitschke M, McDonald D
   Am J Pathol, 2014;184(6):1877-89.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


8. CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-beta.
   Authors: Jablonska J, Wu C, Andzinski L, Leschner S, Weiss S
   Int J Cancer, 2014;134(6):1346-58.
   


9. Type I IFN suppresses Cxcr2 driven neutrophil recruitment into the sensory ganglia during viral infection.
   Authors: Stock A, Smith J, Carbone F
   J Exp Med, 2014;211(5):751-9.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


10. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow

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