抗MIP-1β抗体(Anti-MIP-1β, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse CCL4/MIP-1 beta Ala24-Asn92Accession # Q5QNV9
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects CCL4/MIP-1 beta in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) MIP‑1 beta  and recombinant mouse (rm) MIP-1 alpha is observed. This antibody will not neutralize the biological activity of rmMIP-1 alpha. In the chemotaxis assay this antibody will partially neutralize rhMIP-1 alpha and rhMIP-1 beta at a 10‑30 fold higher IgG concentration.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Immunohistochemistry	1-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize CCL4/MIP‑1 beta -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.2-1.0 µg/mL in the presence of 25 ng/mL Recombinant Mouse CCL4/MIP‑1 beta.

Western Blot
	Detection of Mouse CCL4/MIP‑1 beta by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 μg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse CCL4/MIP‑1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-451-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CCL4/MIP‑1 beta at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	CCL4/MIP‑1 beta in Mouse Small Intestine. CCL4/MIP‑1 beta was detected in perfusion fixed frozen sections of mouse small intestine using Goat Anti-Mouse CCL4/MIP‑1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-451-NA) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to Peyer's patches. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Neutralization
	Chemotaxis Induced by CCL4/MIP‑1 beta and Neutral­ization by Mouse CCL4/MIP‑1 beta  Antibody. Recombinant Mouse CCL4/MIP‑1 beta  (Catalog # 451‑MB) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL4/MIP‑1 beta (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL4/MIP‑1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-451-NA). The ND50 is typically0.2‑1.0 µg/mL.

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Related Product & Information

Entrez Gene IDs	6351 (Human); 20303 (Mouse); 116637 (Rat); 448786 (Canine)
Background	CCL4/MIP-1 beta
background_content	Background: CCL4/MIP-1 beta CCL4, also known as macrophage inflammatory protein 1 beta (MIP-1 beta ), is a 12 kDa beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells (1, 2). CCL4 attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium (3-8). A CCL4 deficiency in mice promotes the development of autoantibodies, possibly as a result of compromised regulatory T cell recruitment (6). CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha (9). The first two N-terminal amino acids can be cleaved from human CCL4 by CD26/DPPIV (10, 11). Both the full length and truncated forms exert biological activity through CCR5, and the truncated form additionally interacts with CCR1 and CCR2 (10). In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor (2). Both forms of CCL4 block HIV-1 infection of T cells by inducing the downregulation of CCR5 (10). Mature mouse CCL4 shares 77% and 86% aa sequence identity with human and rat CCL4, respectively.

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Citations

1. IFN? Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury
   Authors: VE Thaney, AM O'Neill, MM Hoefer, R Maung, AB Sanchez, M Kaul
   Sci Rep, 2017;7(0):46514.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


2. Comprehensive assessment of chemokine expression profiles by flow cytometry.
   Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
   J. Clin. Invest., 2010;120(3):907-23.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process.
   Authors: Wu Y, Li YY, Matsushima K, Baba T, Mukaida N
   J. Immunol., 2008;181(9):6384-93.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


4. Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection.
   Authors: Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A
   J. Exp. Med., 2008;205(9):2075-84.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


5. CCL4 protects from type 1 diabetes by altering islet beta-cell-targeted inflammatory responses.
   Authors: Meagher C, Arreaza G, Peters A, Strathdee CA, Gilbert PA, Mi QS, Santamaria P, Dekaban GA, Delovitch TL
   Diabetes, 2007;56(3):809-17.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


6. CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
   Authors: Cardona AE, Gonzalez PA, Teale JM
   Infect. Immun., 2003;71(5):2634-42.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


7. Role of CC chemokines (macrophage inflammatory protein-1 beta, monocyte chemoattractant protein-1, RANTES) in acute lung injury in rats.
   Authors: Bless NM, Huber-Lang M, Guo RF, Warner RL, Schmal H, Czermak BJ, Shanley TP, Crouch LD, Lentsch AB, Sarma V, Mulligan MS, Friedl HP, Ward PA
   J. Immunol., 2000;164(5):2650-9.
   Species: Rat
   Sample Type: BALF
   Application: ELISA Development

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