抗Osteopontin抗体(Anti-Osteopontin, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Human milk-derived Osteopontin/OPN
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Osteopontin/OPN in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant rat Osteopontin, bovine Osteopontin and recombinant mouse OPN is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human Osteopontin/OPN (Catalog # 1433-OP)
Immunohistochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize Osteopontin/OPN-mediated adhesion of the HEK293 human embryonic kidney cell line. Hu, D.D. et al. (1995) J. Biol. Chem. 270:26232. The Neutralization Dose (ND50) is typically 0.300 - 3.60 µg/mL in the presence of 1 µg/mL Recombinant Human Osteopontin/OPN.

Immunohistochemistry
	Osteopontin/OPN in Human Breast Cancer Tissue. Osteopontin/OPN was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 8 µg/mL Goat Anti-Human Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1433) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the surface of epithelial cells in the intralobular duct. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
	Osteopontin/OPN in Human Breast Cancer Tissue. Osteopontin/OPN was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1433) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Neutralization
	Cell Adhesion Mediated by Osteopontin/OPN and Neutralization by Human Osteopontin/OPN Antibody. Recombinant Human Osteopontin/OPN (Catalog # 1433-OP), immobilized onto a microplate, supports the adhesion of the HEK293 human embryonic kidney cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Osteopontin/OPN (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1433). The ND50 is typically 0.300 - 3.60 µg/mL.

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Related Product & Information

Long Name	Secreted Phosphoprotein 1 [BNSP]
Entrez Gene IDs	6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)
Background	Osteopontin/OPN
background_content	Background: Osteopontin/OPN Osteopontin (OPN, previously also referred to as transformation-associated secreted phosphoprotein, bone sialoprotein I, 2ar, 2B7, early T lymphocyte activation 1 protein, minopotin, calcium oxalate crystal growth inhibitor protein), is a secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein originally isolated from bone matrix (1). Subsequently, OPN has been found in kidney, placenta, blood vessels and various tumor tissues. Many cell types (including macrophages, osteoclasts, activated T cells, fibroblasts, epithelial cells, vascular smooth muscle cells, and natural killer cells) can express OPN in response to activation by cytokines, growth factors or inflammatory mediators. Elevated expression of OPN has also been associated with numerous pathobiological conditions such as atherosclerotic plaques, renal tubulointerstitial fibrosis, granuloma formations in tuberculosis and silicosis, neointimal formation associated with balloon catheterization, metastasizing tumors, and cerebral ischemia. Human OPN cDNA encodes a 314 amino acid (aa) residue precursor protein with a 16 aa residue predicted signal peptide that is cleaved to yield a 298 aa residue mature protein with an integrin binding sequence (RGD), and N- and O-glycosylation sites. By alternative splicing, at least three human OPN isoforms exist. OPN has been shown to bind to different cell types through RGD-mediated interaction with the integrins alpha v beta 1, alpha v beta 3, alpha v beta 5, and non-RGD-mediated interaction with CD44 and the integrins alpha 8 beta 1 or alpha 9 beta 1. OPN exists both as a component of extracellular matrix and as a soluble molecule. Functionally, OPN is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. OPN has also been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Human, mouse, rat, pig, and bovine OPN share from approximately 40-80% amino acid sequence identity. Osteopontin is a substrate for proteolytic cleavage by thrombin, enterokinase, MMP-3, and MMP-7. The functions of OPN in a variety of cell types were shown to be modified as a result of proteolytic cleavage (2, 3).

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Citations

1. Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells
   Authors: AJ Alshawaf, S Viventi, W Qiu, G D'Abaco, B Nayagam, M Erlichster, G Chana, I Everall, J Ivanusic, E Skafidas, M Dottori
   Sci Rep, 2018;8(1):603.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


2. Osteopontin contributes to effective neutrophils recruitment, IL-1? production, and apoptosis in Aspergillus fumigatus keratitis
   Authors: G Zhao, M Hu, C Li, J Lee, K Yuan, G Zhu, C Che
   Immunol. Cell Biol., 2018;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Generation of iPSC-derived limb progenitor-like cells for stimulating phalange regeneration in the adult mouse
   Authors: Y Chen, H Xu, G Lin
   Cell Discov, 2017;3(0):17046.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


4. Osteopontin - Metallothionen I/II Interactions In Experimental Autoimmunune Encephalomyelitis
   Authors: H Jakovac, TG Kezele, S Šu?urovi?, B Mulac-Jeri, B Radoševi?-
   Neuroscience, 2017;0(0):.
   Species: Rat
   Sample Type: Tissue Homogenates
   Application: WB


5. Inhibition of Cellular Adhesion by Immunological Targeting of Osteopontin Neoepitopes Generated through Matrix Metalloproteinase and Thrombin Cleavage.
   Authors: Jurets A, Le Bras M, Staffler G, Stein G, Leitner L, Neuhofer A, Tardelli M, Turkof E, Zeyda M, Stulnig T
   PLoS ONE, 2016;11(2):e0148333.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. Osteopontin promotes the invasive growth of melanoma cells by activating integrin alphavbeta3 and down-regulating tetraspanin CD9.
   Authors: Yin M, Soikkeli J, Jahkola T, Virolainen S, Saksela O, Holtta E
   Am J Pathol, 2014;184(3):842-58.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. Cell stress induces upregulation of osteopontin via the ERK pathway in type II alveolar epithelial cells.
   Authors: Kato, Aki, Okura, Takafumi, Hamada, Chizuru, Miyoshi, Seigo, Katayama, Hitoshi, Higaki, Jitsuo, Ito, Ryoji
   PLoS ONE, 2014;9(6):e100106.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. CD44v6 is a marker of constitutive and reprogrammed cancer stem cells driving colon cancer metastasis.
   Authors: Todaro M, Gaggianesi M, Catalano V, Benfante A, Iovino F, Biffoni M, Apuzzo T, Sperduti I, Volpe S, Cocorullo G, Gulotta G, Dieli F, De Maria R, Stassi G
   Cell Stem Cell, 2014;14(3):342-56.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay


9. Mechanism of hepatitis C virus (HCV)-induced osteopontin and its role in epithelial to mesenchymal transition of hepatocytes.
   Authors: Iqbal J, McRae S, Banaudha K, Mai T, Waris G
   J Biol Chem, 2013;288(52):36994-7009.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


10. Regulation and function of immunosuppressive molecule human leukocyte antigen G5 in human bone tissue.
    Authors: Deschaseaux F, Gaillard J, Langonne A, Chauveau C, Naji A, Bouacida A, Rosset P, Heymann D, De Pinieux G, Rouas-Freiss N, Sensebe L
    FASEB J, 2013;27(8):2977-87.
    Species: Human
    Sample Type: Whole Cells
    Application: IF

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