抗IL-12抗体(Anti-IL-12, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑12
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse IL-12 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing and reducing conditions), this antibody shows less than 10% cross-reactivity with recombinant human IL-12.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse IL‑12 (Catalog # 419-ML)
Neutralization	Measured by its ability to neutralize IL‑12-induced proliferation in PHA-activated mouse splenocytes. Mattner, F. et al. (1993) Eur. J. Immunol. 23:2202. The Neutralization Dose (ND50) is typically 0.01-0.06 µg/mL in the presence of 1 ng/mL Recombinant Mouse IL‑12.

Neutralization

Cell Proliferation Induced by IL‑12 and Neutralization by Mouse IL‑12 Antibody. Recombinant Mouse IL‑12 (Catalog # 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-419-NA). The ND50 is typically 0.01-0.06 µg/mL.

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Related Product & Information

Long Name	Interleukin 12
Entrez Gene IDs	3592 (Human); 16159 (Mouse); 84405 (Rat); 403977 (Canine); 493741 (Feline)
Background	IL-12
background_content	Background: IL-12 Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL‑12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL‑12 activities include the induction of IFN‑ gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. Current evidence indicates that IL‑12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL‑12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent. Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. While mouse IL‑12 is active on both human and mouse cells, human IL‑12 is not active on murine cells. R&D Systems' recombinant mouse IL‑12 preparations were proteolytically cleaved between residues G158 and E159 of the mature p35 subunit. Thus, under reducing conditions, three bands representing the p40 subunit, the p35 R1 - G158 peptide and the p35 E159‑A193 peptide can be observed in SDS-PAGE. The biological activity of this cleaved mouse IL‑12 is comparable to that of the intact human IL‑12.

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Citations

1. Crucial role of OX40/OX40L signaling in a murine model of asthma
   Authors: W Lei, D Zeng, G Liu, Y Zhu, J Wang, H Wu, J Jiang, J Huang
   Mol Med Rep, 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Functional Assay


2. Efficient expression of bioactive murine IL12 as a self-processing P2A polypeptide driven by inflammation-regulated promoters in tumor cell lines.
   Authors: Lorenzo C, Perez-Chacon G, Garaulet G, Mallorquin Z, Zapata J, Rodriguez A
   Cancer Gene Ther, 2015;22(11):542-51.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


3. Viral antigen mediated NKp46 activation of NK cells results in tumor rejection via NK-DC crosstalk.
   Authors: Chinnery, Fay, King, Catherin, Elliott, Tim, Bateman, Andrew R, James, Edward
   Oncoimmunology, 2012;1(6):874-883.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


4. The C5a receptor impairs IL-12-dependent clearance of Porphyromonas gingivalis and is required for induction of periodontal bone loss.
   Authors: Liang S, Krauss JL, Domon H, McIntosh ML, Hosur KB, Qu H, Li F, Tzekou A, Lambris JD, Hajishengallis G
   J. Immunol., 2011;186(2):869-77.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


5. Expression of bioactive single-chain murine IL-12 in transgenic plants.
   Authors: Liu J, Dolan MC, Reidy M, Cramer CL
   J. Interferon Cytokine Res., 2009;28(0):381-92.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


6. Complement receptor 3 blockade promotes IL-12-Mediated Clearance of Porphyromonas gingivalis and Negates Its Virulence In Vivo.
   Authors: Hajishengallis G, Shakhatreh MA, Wang M, Liang S
   J. Immunol., 2007;179(4):2359-67.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


7. Toll-like receptor (TLR)2 and TLR3 synergy and cross-inhibition in murine myeloid dendritic cells.
   Authors: Vanhoutte F, Paget C, Breuilh L, Fontaine J, Vendeville C, Goriely S, Ryffel B, Faveeuw C, Trottein F
   Immunol. Lett., 2007;116(1):86-94.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut

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