抗IL-13抗体(Anti-IL-13, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse IL-13Ser26-Phe131Accession # P20109
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse IL-13 in direct ELISAs and Western blots. In direct ELISAs, approximately 55% cross-reactivity with recombinant rat IL‑13 is observed, and approximately 5% cross-reactivity with recombinant human IL‑13 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse IL‑13 (Catalog # 413-ML)
Immunocytochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize IL‑13-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.05-0.15 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑13.

Neutralization
	Cell Proliferation Induced by IL‑13 and Neutralization by Mouse IL‑13 Antibody. Recombinant Mouse IL‑13 (Catalog # 413-ML) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-413-NA). The ND50 is typically 0.05‑0.15 µg/mL.
Immunocytochemistry
	IL‑13 in Mouse Splenocytes. IL‑13 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse IL‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-413-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Long Name	Interleukin 13
Entrez Gene IDs	3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)
Background	IL-13
background_content	Background: IL-13 IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature mouse IL-13 shares 57%, 75%, and 58% amino acid sequence identity with human, rat, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is overexpressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).

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Citations

1. Lack of interleukin-13 receptor ?1 delays the loss of dopaminergic neurons during chronic stress
   Authors: S Mori, S Sugama, W Nguyen, T Michel, MG Sanna, M Sanchez-Al, R Cintron-Co, G Moroncini, Y Kakinuma, P Maher, B Conti
   J Neuroinflammation, 2017;14(1):88.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. Smoking p66Shc knocked out mice develop respiratory bronchiolitis with fibrosis but not emphysema.
   Authors: Lunghi B, De Cunto G, Cavarra E, Fineschi S, Bartalesi B, Lungarella G, Lucattelli M
   PLoS ONE, 2015;10(3):e0119797.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Not specified


3. Neutrophils prime a long-lived effector macrophage phenotype that mediates accelerated helminth expulsion.
   Authors: Chen, Fei, Wu, Wenhui, Millman, Ariel, Craft, Joshua F, Chen, Eunice, Patel, Nirav, Boucher, Jean L, Urban, Joseph F, Kim, Charles, Gause, William
   Nat Immunol, 2014;15(10):938-46.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Functional Assay


4. Macrophages as IL-25/IL-33-responsive cells play an important role in the induction of type 2 immunity.
   Authors: Yang Z, Grinchuk V, Urban J, Bohl J, Sun R, Notari L, Yan S, Ramalingam T, Keegan A, Wynn T, Shea-Donohue T, Zhao A
   , 2013;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


5. Diesel exhaust particles induce cysteine oxidation and s-glutathionylation in house dust mite induced murine asthma.
   Authors: Lee, Gerald B, Brandt, Eric B, Xiao, Chang, Gibson, Aaron M, Le Cras, Timothy, Brown, Lou Ann, Fitzpatrick, Anne M, Khurana Hershey, Gurjit K
   PLoS ONE, 2013;8(3):e60632.
   Species: Mouse
   Sample Type: BALF
   Application: ELISA Capture


6. Small interfering RNA against transcription factor STAT6 inhibits allergic airway inflammation and hyperreactivity in mice.
   Authors: Darcan-Nicolaisen Y, Meinicke H, Fels G, Hegend O, Haberland A, Kuhl A, Loddenkemper C, Witzenrath M, Kube S, Henke W, Hamelmann E
   J. Immunol., 2009;182(12):7501-8.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


7. Matrix metalloproteinase 8 contributes to solubilization of IL-13 receptor alpha2 in vivo.
   Authors: Chen W, Tabata Y, Gibson AM, Daines MO, Warrier MR, Wills-Karp M, Hershey GK
   J. Allergy Clin. Immunol., 2008;122(3):625-32.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


8. The IL-4/IL-13/Stat6 signalling pathway promotes luminal mammary epithelial cell development.
   Authors: Khaled WT, Read EK, Nicholson SE, Baxter FO, Brennan AJ, Came PJ, Sprigg N, McKenzie AN, Watson CJ
   Development, 2007;134(15):2739-50.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


9. Modulation of airway remodeling and airway inflammation by peroxisome proliferator-activated receptor gamma in a murine model of toluene diisocyanate-induced asthma.
   Authors: Lee KS, Park SJ, Kim SR, Min KH, Jin SM, Lee HK, Lee YC
   J. Immunol., 2006;177(8):5248-57.
   Species: Mouse
   Sample Type: BALF
   Application: WB


10. Level of expression of IL-13R alpha 2 impacts receptor distribution and IL-13 signaling.
    Authors: Daines MO, Tabata Y, Walker BA, Chen W, Warrier MR, Basu S, Hershey GK
    J. Immunol., 2006;176(12):7495-501.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow

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