抗JNK抗体(Anti-JNK, Pan, Human/Mouse/Rat, Rabbit-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant human JNK1Met1-Gln384Accession # P45983
Source	Polyclonal Rabbit IgG
Purification	Antigen Affinity-purified
Specificity	Detects human, mouse, and rat p46 and p54 JNK in Western blots. Detects recombinant human (rh) JNK1, rhJNK2, and rhJNK3.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human/Mouse/Rat JNK by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U937 human histiocytic lymphoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands for JNK were detected at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot
	Detection of recombinant JNK family members by Western Blot. Western blot shows recombinant human JNK1, JNK2, and JNK3 (1 ng/lane). PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	JNK in Human Prostate Cancer Tissue. JNK was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Rabbit Anti-Human/Mouse/Rat JNK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.
Immunohistochemistry
	JNK in Rat Kidney. JNK was detected in perfusion fixed frozen sections of rat kidney using Rabbit Anti-Human/Mouse/Rat JNK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Long Name	C-Jun N-terminal Kinase
Background	JNK
background_content	Background: JNK Members of the MAPK family, the c-Jun N-terminal kinases (JNKs) are activated by environmental stresses and inflammatory cytokines. Ten JNK isoforms are created by alternative splicing of mRNA transcripts derived from three genes: JNK1, JNK2, and JNK3. All JNKs are activated by dual phosphorylation; at T183/Y185 for JNK1 and 2, and T221/Y223 for JNK3. Activated JNKs translocate to the nucleus where they regulate the activity of several transcription factors; including the c-Jun component of AP-1 and ATF-2.

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Citations

1. The c-Jun N-terminal kinase prevents oxidative stress induced by UV and thermal stresses in corals and human cells
   Authors: L Courtial, V Picco, R Grover, Y Cormerais, C Rottier, A Labbe, G Pagès, C Ferrier-Pa
   Sci Rep, 2017;7(0):45713.
   Species: Coral - Stylophora pistillata
   Sample Type: Tissue Homogenates
   Application: WB


2. JNK Promotes Epithelial Cell Anoikis by Transcriptional and Post-translational Regulation of BH3-Only Proteins
   Authors: N Girnius, RJ Davis
   Cell Rep, 2017;21(7):1910-1921.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. White-to-brown metabolic conversion of human adipocytes by JAK inhibition.
   Authors: Moisan A, Lee Y, Zhang J, Hudak C, Meyer C, Prummer M, Zoffmann S, Truong H, Ebeling M, Kiialainen A, Gerard R, Xia F, Schinzel R, Amrein K, Cowan C
   Nat Cell Biol, 2015;17(1):57-67.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Oxidized alpha1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema.
   Authors: Li Z, Alam S, Wang J, Sandstrom CS, Janciauskiene S, Mahadeva R
   Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(2):L388-400.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Distinct role of c-Jun N-terminal kinase isoforms in human neutrophil apoptosis regulated by tumor necrosis factor-alpha and granulocyte-macrophage colony-stimulating factor.
   Authors: Kato T, Noma H, Kitagawa M, Takahashi T, Oshitani N, Kitagawa S
   J. Interferon Cytokine Res., 2008;28(4):235-43.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. Type I IFNs play a role in early resistance, but subsequent susceptibility, to the African trypanosomes.
   Authors: Lopez R, Demick KP, Mansfield JM, Paulnock DM
   J. Immunol., 2008;181(7):4908-17.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB

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