抗Galectin-8抗体(Anti-Galectin-8, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human Galectin-8Met1-Trp317Accession # O00214
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Galectin-8 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) rhGalectin-1 and rhGalectin-3 is observed, and less than 1% cross-reactivity with rhGalectin-2, rhGalectin-4 and rhGalectin-7 is observed.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human Galectin‑8 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Galectin‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Galectin‑8 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	Galectin‑8 in Human Lung. Galectin‑8 was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human Galectin‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit  (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western
	Detection of Human Galectin‑8 by Simple WesternTM. Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑8 at approximately 40 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Galectin‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

目次に戻る

Related Product & Information

Background

Galectin-8

background_content

Background: Galectin-8 The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer. The chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain, and the tandem-repeat Galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1‑4). Galectin-8, also known as prostate carcinoma tumor antigen 1 (PCTA1) in human, is a tandem repeat-type galectin. Prototype (single CRD) isoforms arising through alternate gene splicing have also been identified (5). Galectin-8 is highly expressed in lung carcinomas, certain forms of prostate carcinomas, as well as other tumor cells. It binds to a subset of cell surface integrins to modulate ECM-integrin interactions. As a soluble ligand, Galectin-8 can inhibit cell adhesion (6). Immobilized Galectin-8, however, has also been shown to promote cell adhesion (7). Human and mouse Galectin-8 share approximately 80% amino acid homology (4).

目次に戻る

Citations

1. Phthiocerol dimycocerosates promote access to the cytosol and intracellular burden of Mycobacterium tuberculosis in lymphatic endothelial cells
   Authors: TR Lerner, CJ Queval, A Fearns, U Repnik, G Griffiths, MG Gutierrez
   BMC Biol., 2018;16(1):1.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


2. A Rab20-Dependent Membrane Trafficking Pathway Controls M.�tuberculosis Replication by Regulating Phagosome Spaciousness and Integrity
   Authors: L Schnettger, A Rodgers, U Repnik, RP Lai, G Pei, M Verdoes, RJ Wilkinson, DB Young, MG Gutierrez
   Cell Host Microbe, 2017;21(5):619-628.e5.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


3. K63-Linked Ubiquitination Targets Toxoplasma gondii for Endo-lysosomal Destruction in IFN?-Stimulated Human Cells
   PLoS Pathog, 2016;12(11):e1006027.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


4. Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases.
   Authors: Meunier E, Dick M, Dreier R, Schurmann N, Kenzelmann Broz D, Warming S, Roose-Girma M, Bumann D, Kayagaki N, Takeda K, Yamamoto M, Broz P
   Nature, 2014;509(7500):366-70.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


5. Galectin-8 Promotes Cytoskeletal Rearrangement in Trabecular Meshwork Cells through Activation of Rho Signaling.
   Authors: Diskin S, Chen WS, Cao Z, Gyawali S, Gong H, Soza A, Gonzalez A, Panjwani N
   PLoS ONE, 2012;7(9):e44400.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. Modulation of endothelial cell migration and angiogenesis: a novel function for the "tandem-repeat" lectin galectin-8.
   Authors: Delgado VM, Nugnes LG, Colombo LL, Troncoso MF, Fernandez MM, Malchiodi EL, Frahm I, Croci DO, Compagno D, Rabinovich GA, Wolfenstein-Todel C, Elola MT
   FASEB J., 2011;25(1):242-54.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


7. Galectin-8 interacts with podoplanin and modulates lymphatic endothelial cell functions.
   Authors: Cueni LN, Detmar M
   Exp. Cell Res., 2009;315(10):1715-23.
   Species: Human
   Sample Type: Cell Lysates
   Application: IP


8. The galectin profile of the endothelium: altered expression and localization in activated and tumor endothelial cells.
   Authors: Thijssen VL, Hulsmans S, Griffioen AW
   Am. J. Pathol., 2008;172(2):545-53.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


9. Galectin-8-mediated selective autophagy protects against seeded tau aggregation.
   Authors: Falcon B, Noad J, McMahon H, Randow F, Goedert M
   J Biol Chem, 0;293(7):2438-2451.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC

目次に戻る