抗HGF抗体(Anti-HGF, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HGFAccession # P14210
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human HGF in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse HGF is observed. In Western blots, no cross-reactivity with recombinant canine HGF is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize HGF-induced IL-11 secretion in the Saos‑2 human osteosarcoma cell line. Hjertner, O. et al. (1999) Blood 94:3883.The Neutralization Dose (ND50) is typically 0.01-0.06 μg/mL in the presence of 3 ng/mL Recombinant Human HGF.

Western Blot
	Detection of Recombinant Human HGF by Western Blot. Western blot shows 25 ng of Recombinant Human HGF (Catalog #294‑HG), Recombinant Mouse HGF (Catalog # 2207-HG) and Recombinant Canine HGF (Catalog # 3386-HG). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-294-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for HGF at approximately 30 and 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Immunohistochemistry
	HGF in Human Liver. HGF was detected in formalin fixed paraffin-embedded sections of human liver using 15 µg/mL Goat Anti-Human HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑294‑NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Neutralization
	IL-11 Secretion Induced by HGF and Neutralization by Human HGF Antibody. Recombinant Human HGF induces IL-11 secretion in the Saos‑2 human osteosarcoma cell line. The dose-dependent response (orange line) is measured by the Human IL-11 Duoset (Catalog # DY218). Under these conditions, IL-11 secretion elicited by HGF is neutralized (green line) by increasing concentrations of Goat Anti-Human HGF Antigen Affinity-purified Polyclonal Antibod (Catalog #AF-294-NA). The ND50 is typically 0.01‑0.06 μg/mL.

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Related Product & Information

Long Name	Hepatocyte Growth Factor
Entrez Gene IDs	3082 (Human); 15234 (Mouse); 403441 (Canine)
Background	HGF
background_content	Background: HGF HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity. Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide‑linked HGF with a 60 kDa alpha  and 30 kDa beta chain. Alternate splicing generates human HGF isoforms that lack the proteinase-like domain and different numbers of the Kringle domains. Human HGF shares 91%‑94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET. HGF-dependent c-MET activation is implicated in the development of many human cancers. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis. HGF induces the upregulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription. This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis. HGF can also alter epithelium morphology by the induction of nectin-1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions. In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake. HGF promotes the motility of cardiac stem cells in damaged myocardium.

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Citations

1. Priming with FGF2 stimulates human dental pulp cells to promote axonal regeneration and locomotor function recovery after spinal cord injury
   Authors: K Nagashima, T Miwa, H Soumiya, D Ushiro, T Takeda-Kaw, N Tamaoki, S Ishiguro, Y Sato, K Miyamoto, T Ohno, M Osawa, T Kunisada, T Shibata, KI Tezuka, S Furukawa, H Fukumitsu
   Sci Rep, 2017;7(1):13500.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


2. Matriptase regulates c-Met mediated proliferation and invasion in inflammatory breast cancer
   Authors: GL Zoratti, LM Tanabe, TE Hyland, MJ Duhaime, É Colombo, R Leduc, E Marsault, MD Johnson, CY Lin, J Boerner, JE Lang, K List
   Oncotarget, 2016;7(36):58162-58173.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling.
   Authors: Zoratti G, Tanabe L, Varela F, Murray A, Bergum C, Colombo E, Lang J, Molinolo A, Leduc R, Marsault E, Boerner J, List K
   Nat Commun, 2015;6(0):6776.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: WB


4. Functional mononucleotide repeat polymorphism in the promoter region of HGF is associated with risk and malignant aggressiveness of bladder cancer.
   Authors: Chiba S, Tsuchiya N, Horikawa Y, Narita S, Inoue T, Akihama S, Saito M, Numakura K, Tsuruta H, Huang M, Satoh S, Habuchi T
   Int J Oncol, 2014;44(3):678-84.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - paraffin


5. Activated platelets interfere with recruitment of mesenchymal stem cells to apoptotic cardiac cells via high mobility group box 1/Toll-like receptor 4-mediated down-regulation of hepatocyte growth factor receptor MET.
   Authors: Vogel S, Chatterjee M, Metzger K, Borst O, Geisler T, Seizer P, Muller I, Mack A, Schumann S, Buhring H, Lang F, Sorg R, Langer H, Gawaz M
   J Biol Chem, 2014;289(16):11068-82.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. Spontaneous genomic alterations in a chimeric model of colorectal cancer enable metastasis and guide effective combinatorial therapy.
   Authors: Zhou Y, Rideout W, Bressel A, Yalavarthi S, Zi T, Potz D, Farlow S, Brodeur J, Monti A, Reddipalli S, Xiao Q, Bottega S, Feng B, Chiu M, Bosenberg M, Heyer J
   PLoS ONE, 2014;9(8):e105886.
   


7. Combined targeting of mTOR and c-MET signaling pathways for effective management of epithelioid sarcoma.
   Authors: Imura, Yoshinor, Yasui, Hirohiko, Outani, Hidetats, Wakamatsu, Toru, Hamada, Kenichir, Nakai, Takaaki, Yamada, Shutaro, Myoui, Akira, Araki, Nobuhito, Ueda, Takafumi, Itoh, Kazuyuki, Yoshikawa, Hideki, Naka, Norifumi
   Mol Cancer, 2014;13(0):185.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


8. Associations between hepatocyte growth factor, c-Met, and basic fibroblast growth factor and survival in endometrial cancer patients.
   Authors: Felix AS, Edwards RP, Stone RA
   Br. J. Cancer, 2012;106(12):2004-9.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. Heparanase Plays a Dual Role in Driving Hepatocyte Growth Factor (HGF) Signaling by Enhancing HGF Expression and Activity.
   Authors: Ramani VC, Yang Y, Ren Y
   J. Biol. Chem., 2011;286(8):6490-9.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: IP


10. c-Met-induced epithelial carcinogenesis is initiated by the serine protease matriptase.
    Authors: Szabo R, Rasmussen AL, Moyer AB
    Oncogene, 2011;30(17):2003-16.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB

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