抗RANTES抗体(Anti-RANTES, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human CCL5/RANTESSer24-Ser91Accession # P13501
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human CCL5/RANTES in direct ELISAs. Detects human CCL5/RANTES and mouse CCL5/RANTES Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant canine CCL5/RANTES is observed and approximately 20% cross-reactivity with recombinant cotton rat CCL5/RANTES is observed. In Western blots, no cross-reactivity with recombinant human (rh) CCL3 and rhCCL4 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize CCL5/RANTES-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 0.01 µg/mL Recombinant Human CCL5/RANTES.

Western Blot
	Detection of Recombinant Human and Mouse CCL5/RANTES by Western Blot. Western blot shows 25 ng of Recombinant Human CCL5/RANTES (Catalog # 278-RN), Recombinant Mouse CCL5/RANTES (Catalog # 478-MR), Recombinant Human CCL3/MIP‑1 alpha Isoform LD78a (Catalog # 270-LD), and Recombinant Human CCL4/MIP‑1 beta (Catalog # 271-BME). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-278-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL5/RANTES at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Immunocytochemistry
	CCL5/RANTES in Human PBMCs. CCL5/RANTES was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑278‑NA) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (orange; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunohistochemistry
	CCL5/RANTES in Human Tonsil. CCL5/RANTES was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑278‑NA) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Neutralization
	Chemotaxis Induced by CCL5/RANTES and Neutralization by Human CCL5/RANTES Antibody. Recombinant Human CCL5/RANTES (Catalog # 278‑RN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL5/RANTES (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑278‑NA). The ND50 is typically 0.1‑0.4 µg/mL.

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Related Product & Information

Entrez Gene IDs	6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
Background	CCL5/RANTES
background_content	Background: CCL5/RANTES RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the beta (C-C) chemokine subfamily and is now designated CCL5. It binds and activates the chemokine receptors CCR1, 3 and 5.

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Citations

1. CCR5/CCL5 axis interaction promotes migratory and invasiveness of pancreatic cancer cells
   Authors: SK Singh, MK Mishra, IA Eltoum, S Bae, JW Lillard, R Singh
   Sci Rep, 2018;8(1):1323.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC-Paraffin-Embedded


2. The Ovarian Cancer Chemokine Landscape Is Conducive to Homing of Vaccine-Primed and CD3/CD28-Costimulated T Cells Prepared for Adoptive Therapy.
   Authors: Zsiros E, Duttagupta P, Dangaj D, Li H, Frank R, Garrabrant T, Hagemann I, Levine B, June C, Zhang L, Wang E, Marincola F, Bedognetti D, Powell D, Tanyi J, Feldman M, Kandalaft L, Coukos G
   Clin Cancer Res, 2015;21(12):2840-50.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Not Specified


3. NK cell responses to simian immunodeficiency virus vaginal exposure in naive and vaccinated rhesus macaques.
   Authors: Shang L, Smith A, Duan L, Perkey K, Qu L, Wietgrefe S, Zupancic M, Southern P, Masek-Hammerman K, Reeves R, Johnson R, Haase A
   J Immunol, 2014;193(1):277-84.
   Species: Primate - Macaca mulatta (Rhesus Macaque)
   Sample Type: Whole Tissue
   Application: IHC


4. Lenalidomide inhibits lymphangiogenesis in preclinical models of mantle cell lymphoma.
   Authors: Song K, Herzog B, Sheng M, Fu J, McDaniel J, Chen H, Ruan J, Xia L
   Cancer Res, 2013;73(24):7254-64.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


5. Expression and regulation of chemokines in murine and human type 1 diabetes.
   Authors: Sarkar SA, Lee CE, Victorino F, Nguyen TT, Walters JA, Burrack A, Eberlein J, Hildemann SK, Homann D
   Diabetes, 2012;61(2):436-46.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Irritant-induced migration of Langerhans cells coincides with an IL-10-dependent switch to a macrophage-like phenotype.
   Authors: Ouwehand K, Oosterhoff D, Breetveld M
   J. Invest. Dermatol., 2011;131(2):418-25.
   Species: Human
   Sample Type: Whole Tissue
   Application: Neut


7. Comprehensive assessment of chemokine expression profiles by flow cytometry.
   Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
   J. Clin. Invest., 2010;120(3):907-23.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Tumour necrosis factor alpha stimulates the production of monocyte chemoattractants by extravillous trophoblast cells via differential activation of MAPK pathways.
   Authors: Renaud SJ, Sullivan R, Graham CH
   Placenta, 2009;30(4):313-9.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


9. Disrupting functional interactions between platelet chemokines inhibits atherosclerosis in hyperlipidemic mice.
   Authors: Koenen RR, von Hundelshausen P, Nesmelova IV, Zernecke A, Liehn EA, Sarabi A, Kramp BK, Piccinini AM, Paludan SR, Kowalska MA, Kungl AJ, Hackeng TM, Mayo KH, Weber C
   Nat. Med., 2009;15(1):97-103.
   Species: Human
   Sample Type: Cell Lysates
   Application: IP


10. Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease.
    Authors: Kim MJ, Romero R, Kim CJ, Tarca AL, Chhauy S, LaJeunesse C, Lee DC, Draghici S, Gotsch F, Kusanovic JP, Hassan SS, Kim JS
    J. Immunol., 2009;182(6):3919-27.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen

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