抗Jagged 1抗体(Anti-Jagged 1, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human Jagged 1 Ser32-Asp296Accession # P78504
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Jagged 1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant rat Jagged 1 is observed and less than 1% cross-reactivity with recombinant human Jagged 2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize Jagged 1-induced alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 5 µg/mL Recombinant Human Jagged 1 Fc Chimera.

Western Blot
	Detection of Human Jagged 1 by Western Blot. Western blot shows lysates of Huh‑7 human hepatoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1277) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Jagged 1 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	Jagged 1 in Human Kidney Cancer Tissue. Jagged 1 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1277) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.
Neutralization
	Alkaline Phosphatase Production Induced by Jagged 1 and Neutralization by Human Jagged 1 Antibody. Recombinant Human Jagged 1 Fc Chimera (Catalog # 1277-JG) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human/Mouse/Rat BMP-2 (Catalog # 355-BM) in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human Jagged 1 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Jagged 1 Antibody (Catalog # AF1277). The ND50 is typically 1-5 µg/mL.

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Related Product & Information

Entrez Gene IDs	182 (Human); 29146 (Rat)
Background	Jagged 1
background_content	Background: Jagged 1 Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region. The ECD contains a DSL domain (aa 185‑229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1‑8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9, 10).

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Citations

1. Evolution of cancer stem-like cells in endocrine-resistant metastatic breast cancers is mediated by stromal microvesicles
   Authors: P Sansone, M Berishaj, VK Rajasekhar, C Ceccarelli, Q Chang, A Strillacci, C Savini, L Shapiro, R Bowman, C Mastroleo, S De Carolis, L Daly, A Benito-Mar, F Perna, N Fabbri, JH Healey, E Spisni, M Cricca, D Lyden, M Bonafé, J Bromberg
   Cancer Res, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Bioassay


2. Interleukin-6 Stimulates Defective Angiogenesis.
   Authors: Gopinathan G, Milagre C, Pearce O, Reynolds L, Hodivala-Dilke K, Leinster D, Zhong H, Hollingsworth R, Thompson R, Whiteford J, Balkwill F
   Cancer Res, 2015;75(15):3098-107.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


3. A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment.
   Authors: Kulbe H, Chakravarty P, Leinster DA, Charles KA, Kwong J, Thompson RG, Coward JI, Schioppa T, Robinson SC, Gallagher WM, Galletta L, Salako MA, Smyth JF, Hagemann T, Brennan DJ, Bowtell DD, Balkwill FR
   Cancer Res., 2012;72(1):66-75.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Interleukin-6 as a therapeutic target in human ovarian cancer.
   Authors: Coward J, Kulbe H, Chakravarty P, Leader D, Vassileva V, Leinster DA, Thompson R, Schioppa T, Nemeth J, Vermeulen J, Singh N, Avril N, Cummings J, Rexhepaj E, Jirstrom K, Gallagher WM, Brennan DJ, McNeish IA, Balkwill FR
   Clin. Cancer Res., 2011;17(18):6083-96.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Identification of the surface-accessible, lineage-specific vascular proteome by two-dimensional peptide mapping.
   Authors: Roesli C, Mumprecht V, Neri D, Detmar M
   FASEB J., 2008;22(6):1933-44.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling.
   Authors: Liotta F, Angeli R, Cosmi L, Fili L, Manuelli C, Frosali F, Mazzinghi B, Maggi L, Pasini A, Lisi V, Santarlasci V, Consoloni L, Angelotti ML, Romagnani P, Parronchi P, Krampera M, Maggi E, Romagnani S, Annunziato F
   Stem Cells, 2007;26(1):279-89.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC

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