抗CXCL10抗体(Anti-CXCL10, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human CXCL10/IP-10/CRG-2Val22-Pro98Accession # P02778
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human CXCL10/IP-10/CRG-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant mouse CRG-2, recombinant human (rh) MIG, and rhGRO gamma.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
ELISA	This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human CXCL10/IP‑10/CRG‑2 Monoclonal Antibody (Catalog # MAB2661). This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human CXCL10/IP-10 DuoSet ELISA Kit (Catalog # DY266) for convenient development of a sandwich ELISA or the Human CXCL10/IP-10 Quantikine ELISA Kit (Catalog # DIP100) for a complete optimized ELISA.
Immunohistochemistry	1-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	5-15 µg/mL	See below
Intracellular Staining by Flow Cytometry	2.5 µg/106 cells	Human peripheral blood monocytes treated with Recombinant Human IFN‑ gamma (Catalog # 285‑IF), fixed with paraformaldehyde, and permeabilized with saponin
Neutralization	Measured by its ability to neutralize CXCL10/IP‑10/CRG‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR3. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 0.2 µg/mL Recombinant Human CXCL10/IP‑10/CRG‑2.

Western Blot
	Detection of Human CXCL10/IP‑10/CRG‑2 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CXCL10/IP‑10/CRG‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-266-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CXCL10/IP‑10/CRG‑2 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	CXCL10/IP-10 in Human PBMCs. CXCL10/IP-10 was detected in immersion fixed PHA-treated human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human CXCL10/IP-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-266-NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunohistochemistry
	CXCL10/IP‑10/CRG‑2 in Human Tonsil. CXCL10/IP‑10/CRG‑2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human CXCL10/IP‑10/CRG‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-266-NA) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Neutralization
	Chemotaxis Induced by CXCL10/IP‑10 and Neutralization by Human CXCL10/IP‑10 Antibody. Recombinant Human CXCL10/IP‑10 (Catalog # 266-IP) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL10/IP‑10 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL10/IP‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-266-NA). The ND50 is typically 1-4 µg/mL.
ELISA
	Human CXCL10/IP‑10/CRG‑2 ELISA Standard Curve. Recombinant Human CXCL10/IP‑10/CRG‑2 protein was serially diluted 2-fold and captured by Mouse Anti-Human CXCL10/IP‑10/CRG‑2 Monoclonal Antibody (Catalog # MAB2661) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL10/IP‑10/CRG‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-266-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

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Related Product & Information

Entrez Gene IDs	3627 (Human); 15945 (Mouse)
Background	CXCL10/IP-10/CRG-2
background_content	Background: CXCL10/IP-10/CRG-2 CXCL10 was originally identified as an IFN-gamma -inducible gene in monocytes, fibroblasts, and endothelial cells. It has since been shown that CXCL10 mRNA is also induced by LPS, IL-1 beta, TNF-alpha, IL-12, and viruses. Additional cell types that have been shown to express CXCL10 include activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. CXCL10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human CXCL10, CRG-2, has been cloned and shown to share approximately 67% amino acid sequence identity with human CXCL10. Human CXCL10 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted protein. The amino acid sequence of CXCL10 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes. CXCL10 has been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent antitumor effect. A chemokine receptor specific for CXCL10 and MIG has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

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Citations

1. Differential expression profile of CXCR3 splicing variants is associated with thyroid neoplasia. Potential role in papillary thyroid carcinoma oncogenesis?
   Authors: S Urra, MC Fischer, JR Martínez, L Véliz, P Orellana, A Solar, K Bohmwald, A Kalergis, C Riedel, AH Corvalán, JC Roa, R Fuentealba, CJ Cáceres, M López-Last, A León, N Droppelman, HE González
   Oncotarget, 2018;9(2):2445-2467.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


2. Immunomodulation by mesenchymal stem cells in treating human autoimmune disease-associated lung fibrosis
   Authors: M Liu, X Zeng, J Wang, Z Fu, J Wang, M Liu, D Ren, B Yu, L Zheng, X Hu, W Shi, J Xu
   Stem Cell Res Ther, 2016;7(1):63.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata
   J Immunol, 2016;0(0):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


4. NK cell responses to simian immunodeficiency virus vaginal exposure in naive and vaccinated rhesus macaques.
   Authors: Shang L, Smith A, Duan L, Perkey K, Qu L, Wietgrefe S, Zupancic M, Southern P, Masek-Hammerman K, Reeves R, Johnson R, Haase A
   J Immunol, 2014;193(1):277-84.
   Species: Primate - Macaca mulatta (Rhesus Macaque)
   Sample Type: Whole Tissue
   Application: IHC


5. Expression and regulation of chemokines in murine and human type 1 diabetes.
   Authors: Sarkar SA, Lee CE, Victorino F, Nguyen TT, Walters JA, Burrack A, Eberlein J, Hildemann SK, Homann D
   Diabetes, 2012;61(2):436-46.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Regulation of lentivirus neurovirulence by lipopolysaccharide conditioning: suppression of CXCL10 in the brain by IL-10.
   Authors: Maingat F, Viappiani S, Zhu Y, Vivithanaporn P, Ellestad KK, Holden J, Silva C, Power C
   J. Immunol., 2010;184(3):1566-74.
   Species: Feline
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


7. Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease.
   Authors: Kim MJ, Romero R, Kim CJ, Tarca AL, Chhauy S, LaJeunesse C, Lee DC, Draghici S, Gotsch F, Kusanovic JP, Hassan SS, Kim JS
   J. Immunol., 2009;182(6):3919-27.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen


8. Hyperexpression of NOD2 in intestinal mast cells of Crohn's disease patients: preferential expression of inflammatory cell-recruiting molecules via NOD2 in mast cells.
   Authors: Okumura S, Yuki K, Kobayashi R, Okamura S, Ohmori K, Saito H, Ra C, Okayama Y
   Clin. Immunol., 2009;130(2):175-85.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen


9. Lung myeloid dendritic cells coordinately induce TH1 and TH17 responses in human emphysema.
   Authors: Shan M, Cheng HF, Song LZ
   Sci Transl Med, 2009;1(4):4ra10.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


10. Astrocytes produce interferon-alpha and CXCL10, but not IL-6 or CXCL8, in Aicardi-Goutières syndrome.
    Authors: van Heteren JT, Rozenberg F, Aronica E, Troost D, Lebon P, Kuijpers TW
    Glia, 2008;56(5):568-78.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded

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