抗CXCL5抗体(Anti-CXCL5, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human CXCL5/ENA-78Ala37-Asn114Accession # P42830
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human CXCL5/ENA‑78 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) GCP-2 is observed and less than 5% cross-reactivity with recombinant mouse (rm) KC, recombinant rat CINC-1, rhGRO alpha, rhGRO gamma and rmGCP-2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human CXCL5/ENA‑78 (Catalog # 254-XB)
Immunohistochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize CXCL5/ENA‑78-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.2-1.0 µg/mL in the presence of 0.03 µg/mL Recombinant Human CXCL5/ENA‑78.

Neutralization
	Chemotaxis Induced by CXCL5/ENA‑78 and Neutralization by Human CXCL5/ENA‑78 Antibody. Recombinant Human CXCL5/ENA‑78 (Catalog # 254‑XB) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL5/ENA‑78 (0.03 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL5/ENA‑78 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF254). The ND50 is typically 0.2-1.0 µg/mL.
Immunohistochemistry
	CXCL5/ENA‑78 in Human Breast. CXCL5/ENA‑78 was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-HumanCXCL5/ENA‑78 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF254) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
	CXCL5/ENA‑78 in Human Breast Cancer Tissue. CXCL5/ENA‑78 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human CXCL5/ENA‑78 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF254) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Entrez Gene IDs	6374 (Human)
Background	CXCL5/ENA-78
background_content	Background: CXCL5/ENA-78 CXCL5, also known as epithelial cell-derived neutrophil-activating peptide (ENA-78), is an 8 kDa proinflammatory member of the CXC subfamily of chemokines. Its Glu-Leu-Arg (ELR) motif confers angiogenic properties and distinguishes it from ELR-CXC chemokines which are angiostatic (1‑3). Human CXCL5 shares 57% amino acid (aa) sequence identity with mouse and rat CXCL5. Among other human ELR+ chemokines, it shares 77% aa sequence identity with CXCL6/GCP-2 and35%‑51% with CXCL1/GRO alpha, CXCL2/GRO beta, CXCL3/GRO gamma, CXCL7/NAP-2, and CXCL8/IL-8. Inflammatory stimulation upregulates CXCL5 production in multiple hematopoietic cell types, fibroblasts, endothelial cells, and vascular smooth muscle cells. In vivo, CXCL5 is elevated at sites of inflammation and pulmonary fibrosis where it promotes neutrophil infiltration and activation as well as angiogenesis (3 - 6). Its upregulation contributes to increased vascularization, tumor growth, and metastasis in many cancers (6 - 9). Full length CXCL5 (78 aa) is trimmed at the N-terminus by cathepsin G and chymotrypsin to ENA-74 (74 aa) and ENA-70 (70 aa), with the shortened forms showing increased potency relative to full length CXCL5 (10, 11). CXCL5 exerts its effects primarily through interactions with CXCR2 (6, 12). It also binds duffy antigen receptor for chemokines (DARC), which can limit CXCR2-mediated responses (13, 14).

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Citations

1. Novel adherent CD11b+Gr-1+tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment
   Authors: T Tsubaki, T Kadonosono, S Sakurai, T Shiozawa, T Goto, S Sakai, T Kuchimaru, T Sakamoto, H Watanabe, G Kondoh, S Kizaka-Kon
   Oncotarget, 2018;9(13):11209-11226.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Neutrophil infiltration mediated by CXCL5 accumulation in the laryngeal squamous cell carcinoma microenvironment: A mechanism by which tumour cells escape immune surveillance
   Authors: Li-Ming Lu
   Clin. Immunol., 2016;175(0):34-40.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC-P


3. CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-beta.
   Authors: Jablonska J, Wu C, Andzinski L, Leschner S, Weiss S
   Int J Cancer, 2014;134(6):1346-58.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC- Frozen


4. Overexpression of CXCL5 is associated with poor survival in patients with pancreatic cancer.
   Authors: Li A, King J, Moro A, Sugi MD, Dawson DW, Kaplan J, Li G, Lu X, Strieter RM, Burdick M, Go VL, Reber HA, Eibl G, Hines OJ
   Am. J. Pathol., 2011;178(3):1340-9.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. A CXCL5- and bFGF-dependent effect of PDGF-B-activated fibroblasts in promoting trafficking and differentiation of bone marrow-derived mesenchymal stem cells.
   Authors: Nedeau AE, Bauer RJ, Gallagher K, Chen H, Liu ZJ, Velazquez OC
   Exp. Cell Res., 2008;314(11):2176-86.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


6. Expression and gene polymorphisms of the chemokine CXCL5 in colorectal cancer patients.
   Authors: Dimberg J, Dienus O, Lofgren S, Hugander A, Wagsater D
   Int. J. Oncol., 2007;31(1):97-102.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen


7. Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
   Authors: Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
   J. Immunol., 2002;169(11):6515-21.
   Species: Human
   Sample Type: BALF
   Application: ELISA Development


8. Chemokine CCL15 Mediates Migration of Human Bone Marrow-Derived Mesenchymal Stem Cells Toward Hepatocellular Carcinoma.
   Authors: Gao Y, Zhou Z, Lu S, Huang X, Zhang C, Jiang R, Yao A, Sun B, Wang X
   Stem Cells, 0;34(4):1112-22.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut

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