抗IL-15 Receptor α抗体(Anti-IL-15 Receptor α, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant humanIL‑15 R alpha Ile31-Thr172Accession # EAW86418
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human IL-15 R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant humanIL‑2 R gamma is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human IL‑15 R alpha Fc Chimera (Catalog # 147-IR)
Flow Cytometry	2.5 µg/106 cells	Human peripheral blood mononuclear cells treated with PHA
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Neutralization	Measured by its ability to neutralize IL‑15 R alpha -mediated inhibition of proliferation in the CTLL‑2 mouse cytotoxic T cell line. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 30 ng/mL Recombinant Human IL‑15 R alpha Fc Chimera and 2 ng/mL Recombinant Human IL‑15.

Immunohistochemistry
	IL‑15 R alpha in Human Prostate Cancer Tissue. IL‑15 R alpha was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 15 µg/mL Goat Anti-Human IL‑15 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF247) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Neutralization
	IL‑15 R alpha Inhibition of IL‑15-dependent Cell Proliferation and Neutralization by Human IL‑15 R alpha Antibody. Recombinant Human IL‑15 R alpha Fc Chimera (Catalog #147‑IR) inhibits Recombinant Human IL‑15 (Catalog #247‑IL) induced proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL‑15 (2 ng/mL) activity elicited by Recombinant Human IL‑15 R alpha Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑15 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF247). The ND50 is typically1‑4 µg/mL.

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Related Product & Information

Long Name	Interleukin 15 Receptor alpha
Entrez Gene IDs	3601 (Human); 16169 (Mouse)
Background	IL-15 R alpha
background_content	Background: IL-15 R alpha Interleukin 15 receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells.IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Eight isoforms ofIL‑15 R alpha mRNA have been identified resulting from alternative splicing events involving different exons. The exclusion of exon 2 results in an IL-15 R alpha isoform that does not bind IL-15. Human IL-15 R alpha DE3 cDNA encodes a 267 amino acid (aa) protein that contains a 30 aa signal sequence, a 175 aa extracellular region containing one N-linked glycosylation site, a 21 aa transmembrane domain, and a 41 aa cytoplasmic tail. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha, IL-2 R beta, and IL-2 R gamma c, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF-alpha -mediated apoptosis in fibroblasts by competing with TNF RI for TRAF2 binding.

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Citations

1. Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways.
   Authors: Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D
   J Immunol, 2015;195(6):2818-28.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC Fluorescence


2. Intramuscular delivery of heterodimeric IL-15 DNA in macaques produces systemic levels of bioactive cytokine inducing proliferation of NK and T cells.
   Authors: Bergamaschi C, Kulkarni V, Rosati M, Alicea C, Jalah R, Chen S, Bear J, Sardesai N, Valentin A, Felber B, Pavlakis G
   Gene Ther, 2015;22(1):76-86.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Regulation of CD4(+)NKG2D(+) Th1 cells in patients with metastatic melanoma treated with sorafenib: role of IL-15Ralpha and NKG2D triggering.
   Authors: Romero A, Chaput N, Poirier-Colame V, Rusakiewicz S, Jacquelot N, Chaba K, Mortier E, Jacques Y, Caillat-Zucman S, Flament C, Caignard A, Messaoudene M, Auperin A, Vielh P, Dessen P, Porta C, Mateus C, Ayyoub M, Valmori D, Eggermont A, Robert C, Zitvogel L
   Cancer Res, 2014;74(1):68-80.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


4. Interleukin-15 enhances cellular proliferation and upregulates CNS homing molecules in pre-B acute lymphoblastic leukemia.
   Authors: Williams M, Yousafzai Y, Cox C, Blair A, Carmody R, Sai S, Chapman K, McAndrew R, Thomas A, Spence A, Gibson B, Graham G, Halsey C
   Blood, 2014;123(20):3116-27.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. Paracrine and transpresentation functions of IL-15 are mediated by diverse splice versions of IL-15Ralpha in human monocytes and dendritic cells.
   Authors: Muller J, Waldmann T, Kruhlak M, Dubois S
   J Biol Chem, 2012;287(48):40328-38.
   Species: Human
   Sample Type: Cell Lysates
   Application: ELISA Development


6. Interleukin-15 plays a central role in human kidney physiology and cancer through the gamma c signaling pathway.
   Authors: Giron-Michel J, Azzi S, Khawam K, Mortier E, Caignard A, Devocelle A, Ferrini S, Croce M, Francois H, Lecru L, Charpentier B, Chouaib S, Azzarone B, Eid P
   PLoS ONE, 2012;7(2):e31624.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


7. Increased IL-15 production is associated with higher susceptibility of memory CD4 T cells to simian immunodeficiency virus during acute infection.
   Authors: Eberly MD, Kader M, Hassan W, Rogers KA, Zhou J, Mueller YM, Mattapallil MJ, Piatak M, Lifson JD, Katsikis PD, Roederer M, Villinger F, Mattapallil JJ
   J. Immunol., 2009;182(3):1439-48.
   Species: Primate - Macaca mulatta (Rhesus Macaque)
   Sample Type: Whole Cells
   Application: Flow


8. EBV-associated mononucleosis does not induce long-term global deficit in T-cell responsiveness to IL-15.
   Authors: Giron-Michel J, Menard F, Negrini S, Devocelle A, Azzarone B, Besson C
   Blood, 2009;113(19):4541-7.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


9. Human T cells constitutively express IL-15 that promotes ex vivo T cell homeostatic proliferation through autocrine/juxtacrine loops.
   Authors: Miranda-Carus ME, Benito-Miguel M, Llamas MA, Balsa A, Martin-Mola E
   J. Immunol., 2005;175(6):3656-62.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


10. Renal cells express a functional interleukin-15 receptor.
    Authors: Tejman-Yarden N, Zlotnik M, Lewis E, Etzion O, Chaimovitz C, Douvdevani A
    Nephrol. Dial. Transplant., 2005;20(3):516-23.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

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