抗FGF Basic抗体(Anti-FGF Basic, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Bovine brain-derived FGF basic
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human and bovine FGF basic in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant mouse FGF basic is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human FGF basic 146 aa (Catalog # 233-FB)
Immunohistochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize FGF basic-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND50) is typically 0.08-0.4 µg/mL in the presence of 0.5 ng/mL Bovine FGF basic.

Immunohistochemistry
	FGF basic in Human Placenta. FGF basic was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human FGF basic Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑233‑NA) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to trophoblast cells in chorionic villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Neutralization
	Cell Proliferation Induced by FGF basic and Neutralization by Human FGF basic Antibody. Bovine FGF basic (Catalog # 133-FB) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Bovine FGF basic (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF basic Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-233-NA). The ND50 is typically 0.08‑0.4 µg/mL.

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Related Product & Information

Long Name	Fibroblast Growth Factor basic
Entrez Gene IDs	2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
Background	FGF basic
background_content	Background: FGF basic FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35 ‑ 60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. This factor contains four cysteine residues, but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. A variety of forms of FGF basic are produced as a result of N-terminal extensions. These extensions affect localization of FGF basic in cellular compartments but do not affect biological activity. Binding of FGF to heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. FGF acidic and basic appear to bind to the same high affinity receptors and show a similar range of biological activities. FGF basic stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. FGF basic induces neuron differentiation, survival, and regeneration. FGF basic also modulates embryonic development and differentiation. These observed in vitro functions of FGF basic suggest FGF basic may play a role in vivo in the modulation of such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Additionally, FGF basic may participate in the production of a variety of pathological conditions resulting from excessive cell proliferation and excessive angiogenesis.

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Citations

1. Endothelial heparan sulfate 6-o-sulfation levels regulate angiogenic responses of endothelial cells to fibroblast growth factor 2 and vascular endothelial growth factor.
   Authors: Ferreras, Cristina, Rushton, Graham, Cole, Claire L, Babur, Muhammad, Telfer, Brian A, van Kuppevelt, Toin H, Gardiner, John M, Williams, Kaye J, Jayson, Gordon C, Avizienyte, Egle
   J Biol Chem, 2102;287(43):36132-46.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen


2. Multicolor quantitative confocal imaging cytometry
   Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
   Nat. Methods, 2018;15(1):39-46.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


3. Tumor-associated B-cells induce tumor heterogeneity and therapy resistance
   Authors: R Somasundar, G Zhang, M Fukunaga-K, M Perego, C Krepler, X Xu, C Wagner, D Hristova, J Zhang, T Tian, Z Wei, Q Liu, K Garg, J Griss, R Hards, M Maurer, C Hafner, M Mayerhöfer, G Karanikas, A Jalili, V Bauer-Pohl, F Weihsengru, K Rappersber, J Koller, R Lang, C Hudgens, G Chen, M Tetzlaff, L Wu, DT Frederick, RA Scolyer, GV Long, M Damle, C Ellingswor, L Grinman, H Choi, BJ Gavin, M Dunagin, A Raj, N Scholler, L Gross, M Beqiri, K Bennett, I Watson, H Schaider, MA Davies, J Wargo, BJ Czerniecki, L Schuchter, D Herlyn, K Flaherty, M Herlyn, SN Wagner
   Nat Commun, 2017;8(1):607.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. Pericytes impair capillary blood flow and motor function after chronic spinal cord injury
   Authors: Y Li, AM Lucas-Osma, S Black, MV Bandet, MJ Stephens, R Vavrek, L Sanelli, KK Fenrich, AF Di Narzo, S Dracheva, IR Winship, K Fouad, DJ Bennett
   Nat. Med., 2017;0(0):.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC


5. Peroxynitrite upregulates angiogenic factors VEGF-A, BFGF, and HIF-1alpha in human corneal limbal epithelial cells.
   Authors: Ashki N, Chan A, Qin Y, Wang W, Kiyohara M, Lin L, Braun J, Wadehra M, Gordon L
   Invest Ophthalmol Vis Sci, 2014;55(3):1637-46.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. Inhibition of PAI-1 induces neutrophil-driven neoangiogenesis and promotes tissue regeneration via production of angiocrine factors in mice.
   Blood, 2012;119(26):6382-93.
   Species: Mouse
   Sample Type: In Vivo
   Application: In Vivo


7. The fibroblast-derived paracrine factor neuregulin-1 has a novel role in regulating the constitutive color and melanocyte function in human skin.
   Authors: Choi W, Wolber R, Gerwat W
   J. Cell. Sci., 2010;123(0):3102-11.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


8. IFATS Series: FGF-2-induced HGF Secretion By Adipose-Derived Stromal Cells Inhibits Post-Injury Fibrogenesis Through A JNK-Dependent Mechanism.
   Authors: Suga H, Eto H, Shigeura T, Inoue K, Aoi N, Kato H, Nishimura S, Manabe I, Gonda K, Yoshimura K
   Stem Cells, 2008;0(0):.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


9. A CXCL5- and bFGF-dependent effect of PDGF-B-activated fibroblasts in promoting trafficking and differentiation of bone marrow-derived mesenchymal stem cells.
   Authors: Nedeau AE, Bauer RJ, Gallagher K, Chen H, Liu ZJ, Velazquez OC
   Exp. Cell Res., 2008;314(11):2176-86.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


10. Pancreatic stellate cells: partners in crime with pancreatic cancer cells.
    Authors: Vonlaufen A, Joshi S, Qu C, Phillips PA, Xu Z, Parker NR, Toi CS, Pirola RC, Wilson JS, Goldstein D, Apte MV
    Cancer Res., 2008;68(7):2085-93.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut

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