抗IL-6 Receptor抗体(Anti-IL-6 Receptor, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑6 R alpha Leu20-Asp358Accession # P08887
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human IL-6 R alpha in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	See below
Neutralization	Measured by its ability to neutralize IL‑6 R alpha -mediated inhibition of proliferation in the M1 mouse myeloid leukemia cell line. The Neutralization Dose (ND50) is typically 0.2-0.4 µg/mL in the presence of 30 ng/mL Recombinant Human IL‑6 R alpha and 30 ng/mL Recombinant Human IL‑6.

Western Blot
	Detection of Recombinant Human IL‑6 R alpha by Western Blot. Western blot shows 5 ng of Recombinant Human IL‑6 R alpha (Catalog # 227-SR), Recombinant Mouse IL‑6 R alpha (Catalog # 1830-SR) and Recombinant Rat IL-6 R. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human IL‑6 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-227-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL‑6 R alpha at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Neutralization
	IL‑6 R alpha  Enhancement of IL‑6‑dependent Inhibition of Cell Proliferation and Neutralization by Human IL‑6 R alpha  Antibody. Recombinant Human IL‑6 R alpha (Catalog # 227‑SR) enhances Recombinant Human IL‑6 (Catalog #206‑IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL‑6 (30 ng/mL) activity elicited by Recombinant Human IL‑6 R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑6 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog #AF‑227‑NA). The ND50 is typically 0.2‑0.4 µg/mL.

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Related Product & Information

Long Name	Interleukin 6 Receptor alpha
Entrez Gene IDs	3570 (Human); 16194 (Mouse)
Background	IL-6 R alpha
background_content	Background: IL-6 R alpha The multi-functional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4). A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha and is about 50kDa in size. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).

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Citations

1. An autoinflammatory neurological disease due to interleukin 6 hypersecretion.
   Authors: Salsano, Ettore, Rizzo, Ambra, Bedini, Gloria, Bernard, Loris, Dall'olio, Valentin, Volorio, Sara, Lazzaroni, Monica, Ceccherini, Isabella, Lazarevic, Dejan, Cittaro, Davide, Stupka, Elia, Paterra, Rosina, Farina, Laura, Savoiardo, Mario, Pareyson, Davide, Sciacca, Francesc
   J Neuroinflammation, 2013;10(0):29.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1beta, IL-23, and TNF-like 1A expression by monocytes.
   Authors: Cunin P, Caillon A, Corvaisier M, Garo E, Scotet M, Blanchard S, Delneste Y, Jeannin P
   J. Immunol., 2011;186(7):4175-82.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
   Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
   Dig. Dis. Sci., 2007;52(10):2775-83.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


4. Alterations in Janus kinase (JAK)-signal transducers and activators of transcription (STAT) signaling in patients with end-stage dilated cardiomyopathy.
   Authors: Podewski EK, Hilfiker-Kleiner D, Hilfiker A, Morawietz H, Lichtenberg A, Wollert KC, Drexler H
   Circulation, 2003;107(6):798-802.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: WB


5. Protection of CLL B cells by a follicular dendritic cell line is dependent on induction of Mcl-1.
   Authors: Pedersen IM, Kitada S, Leoni LM, Zapata JM, Karras JG, Tsukada N, Kipps TJ, Choi YS, Bennett F, Reed JC
   Blood, 2002;100(5):1795-801.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut

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