抗GM-CSF抗体(Anti-GM-CSF, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human GM-CSFAla18-Glu144Accession # P04141
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human GM‑CSF in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse GM‑CSF is observed. Neutralizes the biological activity of both recombinant human GM‑CSF and natural human GM‑CSF.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human GM-CSF (Catalog # 215-GM)
Neutralization	Measured by its ability to neutralize GM‑CSF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.08-0.16 µg/mL in the presence of 0.5 ng/mL Recombinant Human GM‑CSF.

Neutralization

Cell Proliferation Induced by GM‑CSF and Neutralization by Human GM‑CSF Antibody. Recombinant Human GM‑CSF (Catalog # 215-GM) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human GM‑CSF (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human GM‑CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑215‑NA). The ND50 is typically 0.08-0.16 µg/mL.

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Related Product & Information

Long Name	Granulocyte Macrophage Growth Factor
Entrez Gene IDs	1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)
Background	GM-CSF
background_content	Background: GM-CSF GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3‑5). It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core of four bundled alpha ‑helices (8‑12). Mature human GM-CSF shares 63%‑70% amino acid sequence identity with canine, feline, porcine, and rat GM-CSF and 54% with mouse GM-CSF. GM-CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF R alpha /CD116 and the signal transducing common beta  chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5 (13, 14). In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF R alpha (15). Human GM-CSF is active on canine and feline cells but not on murine cells (16‑18).

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Citations

1. Decidual GM-CSF is a critical common intermediate necessary for thrombin and TNF induced in-vitro fetal membrane weakening.
   Authors: Kumar D, Moore R, Nash A, Springel E, Mercer B, Philipson E, Mansour J, Moore J
   Placenta, 2014;35(12):1049-56.
   Species: Human
   Sample Type: Whole Tissue
   Application: Neut


2. Low concentrations of recombinant granulocyte macrophage-colony stimulating factor derived from Chinese hamster ovary cells augments long-term bioactivity with delayed clearance in vitro.
   Authors: Hashimoto A, Tanaka T, Itoh Y, Yamagata A, Kitamura N, Tazawa R, Nakagaki K, Nakata K
   Cytokine, 2014;68(2):118-26.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Apoptotic neutrophils release macrophage migration inhibitory factor upon stimulation with tumor necrosis factor-alpha.
   Authors: Daryadel A, Grifone RF, Simon HU, Yousefi S
   J. Biol. Chem., 2006;281(37):27653-61.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


4. Secretory IgA induces antigen-independent eosinophil survival and cytokine production without inducing effector functions.
   Authors: Bartemes KR, Cooper KM, Drain KL, Kita H
   J. Allergy Clin. Immunol., 2005;116(4):827-35.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. Toxoplasma gondii induces granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor secretion by human fibroblasts: implications for neutrophil apoptosis.
   Authors: Channon JY, Miselis KA, Minns LA, Dutta C, Kasper LH
   Infect. Immun., 2002;70(11):6048-57.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. Bleomycin stimulates lung fibroblast and epithelial cell lines to release eosinophil chemotactic activity.
   Authors: Sato E, Koyama S
   Eur. Respir. J., 2000;16(5):951-8.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut

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