抗G-CSF抗体(Anti-G-CSF, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human G-CSFThr31-Pro204Accession # NP_757373
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human G-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 30% cross-reactivity with recombinant mouse G-CSF and recombinant canine G-CSF is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	See below
Neutralization	Measured by its ability to neutralize G‑CSF-induced proliferation in the M‑NFS‑60 mouse myeloid cell line. Shirafuji, N.et al. (1989) Exp. Hematol. 17:116. The Neutralization Dose (ND50) is typically 0.02-0.1 µg/mL in the presence of 0.125 ng/mL Recombinant Human G‑CSF.

Western Blot
	Detection of Recombinant Human G‑CSF by Western Blot. Western blot shows 25 ng of Recombinant Human G‑CSF (Catalog # 214-CS) and Recombinant Mouse G‑CSF (Catalog # 414-CS). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human G‑CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-214-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for G‑CSF at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Neutralization
	Cell Proliferation Induced by G‑CSF and Neutralization by Human G‑CSF Antibody. Recombinant Human G‑CSF (Catalog # 214-CS) stimulates proliferation in the NFS‑60 mouse  myeloid cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human G‑CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human G‑CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-214-NA). The ND50 is typically 0.02-0.1 µg/mL.

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Related Product & Information

Long Name	Granulocyte Colony Stimulating Factor
Entrez Gene IDs	1440 (Human); 12985 (Mouse)
Background	G-CSF
background_content	Background: G-CSF G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1, or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid precursor proteins, have been isolated. Both proteins have a 30 amino acid signal peptide and have identical amino acid sequences except for a three amino acid insertion (deletion) at the 35th amino acid residue from the N-terminus of the mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity. In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis.

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Citations

1. Enhanced non-viral gene delivery by coordinated endosomal release and inhibition of ?-tubulin deactylase
   Nucleic Acids Res, 2016;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Does granulocyte colony-stimulating factor ameliorate the proinflammatory response in human meningococcal septic shock?
   Authors: Rojahn A, Brusletto B, Ovstebo R, Haug KB, Kierulf P, Brandtzaeg P
   Crit. Care Med., 2008;36(9):2583-9.
   Species: Human
   Sample Type: Plasma
   Application: Immunodepletion


3. Proinflammatory mediators elicit secretion of the intracellular B-lymphocyte stimulator pool (BLyS) that is stored in activated neutrophils: implications for inflammatory diseases.
   Authors: Scapini P, Carletto A, Nardelli B, Calzetti F, Roschke V, Merigo F, Tamassia N, Pieropan S, Biasi D, Sbarbati A, Sozzani S, Bambara L, Cassatella MA
   Blood, 2005;105(2):830-7.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. Enhanced circulating half-life and hematopoietic properties of a human granulocyte colony-stimulating factor/immunoglobulin fusion protein.
   Authors: Cox GN, Smith DJ, Carlson SJ, Bendele AM, Chlipala EA, Doherty DH
   Exp. Hematol., 2004;32(5):441-9.
   Species: Human
   Sample Type: Recombinant Protein
   Application: WB


5. Toxoplasma gondii induces granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor secretion by human fibroblasts: implications for neutrophil apoptosis.
   Authors: Channon JY, Miselis KA, Minns LA, Dutta C, Kasper LH
   Infect. Immun., 2002;70(11):6048-57.
   Species: Human
   Sample Type:
   Application: Neut

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