抗Galectin-1抗体(Anti-Galectin-1, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human Galectin‑1Ala2-Asp135Accession # P09382
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Galectin-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse (rm) Galectin-1 is observed and less than 5% cross-reactivity with recombinant human (rh) Galectin‑2, rhGalectin‑3, rhGalectin-4, rhGalectin-7, rhGalectin-8, and rmGalectin-9 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	See below
Simple Western	1 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	2.5 µg/106 cells	See below

Western Blot
	Detection of Human Galectin‑1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Galectin‑1 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	Galectin‑1 in Human Prostate Cancer Tissue. Galectin‑1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 1.7 µg/mL Goat Anti-Human Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
	Galectin‑1 in Human Prostate Cancer Tissue. Galectin‑1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of stromal cells and nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Intracellular Staining by Flow Cytometry
	Detection of Galectin‑1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Normal Goat IgG Control (Catalog # AB-108-C) or (B) Goat Anti-Human Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Simple Western
	Detection of Human Galectin‑1 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑1 at approximately 17 kDa (as indicated) using 1 µg/mL of Goat Anti-Human Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

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Related Product & Information

Entrez Gene IDs	3956 (Human); 16852 (Mouse)
Background	Galectin-1
background_content	Background: Galectin-1 The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins. Galectin-1, also known as L-14, BHL and galaptin, is a monomeric or homodimeric prototype galectin that is expressed in a variety of cells and tissues including muscle, heart, liver, prostate, lymph nodes, spleen, thymus, placenta, testis, retina, macrophages, B cells, T cells, dendritic cells, and tumor cells. It preferentially binds laminin, fibronectin, 90K/Mac-2BP, CD45, CD43, CD7, CD2, CD3, and ganglioside GM1. Galectin-1 modulates cell growth and proliferation, either positively or negatively, depending on the cell type and activation status. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes. It modulates cytokine secretion by inducing Th2 type cytokines and inhibiting pro-inflammatory cytokine production. Galectin-1 can also modulate cell-cell as well as cell-matrix interactions and depending on the cell type and developmental stage, promote cell attachment or detachment. Galectin-1 has immunosuppressive and anti-inflammatory properties and has been shown to suppress acute and chronic inflammation and autoimmunity. Human and mouse galectin-1 share about 88% amino acid sequence similarity.

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Citations

1. Treatment of B-cell precursor acute lymphoblastic leukemia with the Galectin-1 inhibitor PTX008
   Authors: H Paz, EJ Joo, CH Chou, F Fei, KH Mayo, H Abdel-Azim, H Ghazarian, J Groffen, N Heisterkam
   J. Exp. Clin. Cancer Res., 2018;37(1):67.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Serum galectin-1 in patients with multiple myeloma: associations with survival, angiogenesis, and biomarkers of macrophage activation
   Authors: MN Andersen, M Ludvigsen, N Abildgaard, I Petruskevi, R Hjortebjer, M Bjerre, B Honoré, HJ Møller, NF Andersen
   Onco Targets Ther, 2017;10(0):1977-1982.
   Species: Human
   Sample Type: Serum
   Application: ELISA Capture


3. Galectins-1, -3, and -7 Are Prognostic Markers for Survival of Ovarian Cancer Patients
   Authors: H Schulz, E Schmoeckel, C Kuhn, S Hofmann, D Mayr, S Mahner, U Jeschke
   Int J Mol Sci, 2017;18(6):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


4. Prototype and Chimera-Type Galectins in Placentas with Spontaneous and Recurrent Miscarriages
   Int J Mol Sci, 2016;17(5):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


5. Melanoma Cell Galectin-1 Ligands Functionally Correlate with Malignant Potential.
   Authors: Yazawa E, Geddes-Sweeney J, Cedeno-Laurent F, Walley K, Barthel S, Opperman M, Liang J, Lin J, Schatton T, Laga A, Mihm M, Qureshi A, Widlund H, Murphy G, Dimitroff C
   J Invest Dermatol, 2015;135(7):1849-62.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Graves' disease is associated with a defective expression of the immune regulatory molecule galectin-9 in antigen-presenting dendritic cells.
   Authors: Leskela S, Serrano A, de la Fuente H, Rodriguez-Munoz A, Ramos-Levi A, Sampedro-Nunez M, Sanchez-Madrid F, Gonzalez-Amaro R, Marazuela M
   PLoS ONE, 2015;10(4):e0123938.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Tonsil-derived mesenchymal stem cells alleviate concanavalin A-induced acute liver injury.
   Authors: Ryu K, Kim S, Kim Y, Woo S, Sung S, Kim H, Jung S, Jo I, Park J
   Exp Cell Res, 2014;326(1):143-54.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


8. Galectin-1-mediated cell death is increased by CD30-induced signaling in anaplastic large cell lymphoma cells but not in Hodgkin lymphoma cells.
   Authors: Suzuki O, Hirsch B, Abe M, Durkop H, Stein H
   Lab. Invest., 2012;92(2):191-9.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. Sialylation of beta1 integrins blocks cell adhesion to galectin-3 and protects cells against galectin-3-induced apoptosis.
   Authors: Zhuo Y, Chammas R, Bellis SL
   J. Biol. Chem., 2008;283(32):22177-85.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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