抗TNF Receptor Ⅲ抗体(Anti-TNF Receptor Ⅲ, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse Lymphotoxin beta R/TNFRSF3Ser28-Pro218Accession # P50284
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse Lymphotoxin beta R/TNFRSF3 in direct ELISA and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) LT beta R is observed, and less than 1% cross-reactivity with recombinant mouse (rm) 4-1BB, rmCD27, rmOPG, rmRANK, rmCD30, rmCD40, rhDR6, rmTNF RI, rmTNF RII, rmEDAR, rmFas and rmGITR is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse Lymphotoxin  beta R/TNFRSF3
Flow Cytometry	0.25 µg/106 cells	See below
Immunohistochemistry	5-15 µg/mL	Perfusion fixed frozen sections of mouse thymus
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

Detection of Lymphotoxin  beta R/TNFRSF3 in NIH‑3T3 Mouse Cell Line by Flow Cytometry. NIH‑3T3 mouse embryonic fibroblast cell line was stained with Goat Anti-Mouse Lymphotoxin  beta R/TNFRSF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1008, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Long Name	Lymphotoxin beta Receptor
Background	Lymphotoxin beta R/TNFRSF3
background_content	Background: Lymphotoxin beta R/TNFRSF3 Lymphotoxin beta R is a member of the TNF receptor superfamily and is now designated TNFRSF3. Lymphotoxin beta R transduces signals following binding of LIGHT or the heterotrimeric Lymphotoxins LT alpha 1/ beta 2 or LT alpha 2/ beta 1. It plays a critical role in controlling cellular immune functions and lymphoid organogenesis.

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Citations

1. Fibroblast reticular cells engineer a blastema extracellular network during digit tip regeneration in mice
   Authors: L Marrero, J Simkin, M Sammarco, K Muneoka
   Regeneration (Oxf), 2017;4(2):69-84.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Bioassay


2. A dendritic-cell-stromal axis maintains immune responses in lymph nodes.
   Authors: Kumar V, Dasoveanu D, Chyou S, Tzeng T, Rozo C, Liang Y, Stohl W, Fu Y, Ruddle N, Lu T
   Immunity, 2015;42(4):719-30.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


3. Autotaxin produced by stromal cells promotes LFA-1-independent and Rho-dependent interstitial T cell motility in the lymph node paracortex.
   Authors: Katakai T, Kondo N, Ueda Y, Kinashi T
   J Immunol, 2014;193(2):617-26.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Functional Assay


4. TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
   Authors: Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM
   J. Clin. Invest., 2009;119(10):2954-64.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


5. CXCL13 production by an established lymph node stromal cell line via lymphotoxin-beta receptor engagement involves the cooperation of multiple signaling pathways.
   Authors: Suto H, Katakai T, Sugai M, Kinashi T, Shimizu A
   Int. Immunol., 2009;21(4):467-76.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


6. Organizer-like reticular stromal cell layer common to adult secondary lymphoid organs.
   Authors: Katakai T, Suto H, Sugai M, Gonda H, Togawa A, Suematsu S, Ebisuno Y, Katagiri K, Kinashi T, Shimizu A
   J. Immunol., 2008;181(9):6189-200.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Bioassay


7. Lymph node fibroblastic reticular cells construct the stromal reticulum via contact with lymphocytes.
   Authors: Katakai T, Hara T, Sugai M, Gonda H, Shimizu A
   J. Exp. Med., 2004;200(6):783-95.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

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