抗G-CSF抗体(Anti-G-CSF, Mouse-Mono, APC antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information

価格

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Product Details

Species Reactivity	Human
Label	Allophycocyanin
Immunogen	E. coli-derived recombinant human G-CSFThr31-Pro204Accession # NP_757373
Source	Monoclonal Mouse IgG1 Clone # 5008
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human G-CSF in flow cytometry.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry
	Detection of G‑CSF in Human Macrophages by Flow Cytometry. Human macrophages treated with LPS, TNF-alpha and IL-1 beta was stained with Mouse Anti-Human G‑CSF APC‑conjugated Monoclonal Antibody (Catalog # IC2141A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Long Name	Granulocyte Colony Stimulating Factor
Background	G-CSF
background_content	Background: G-CSF G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1, or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid precursor proteins, have been isolated. Both proteins have a 30 amino acid signal peptide and have identical amino acid sequences except for a three amino acid insertion (deletion) at the 35th amino acid residue from the N-terminus of the mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity. In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis.

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