抗Human MMP-9抗体(Anti-Human MMP-9 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Chinese hamster ovary cell line CHO-derived recombinant human MMP-9
Source	Monoclonal Mouse IgG1 Clone # 4H3
Purification	Protein A or G purified from ascites
Specificity	Detects human MMP-9 in Western blots. In Western blots, reactivity with the pro (92 kDa), active (82 kDa), and C-terminal truncated (65 kDa) forms of recombinant human (rh) MMP-9 is observed. Also in Western blots, 20% cross-reactivity with rhMMP-2, 5% cross‑reactivity with rhMMP-1, and no cross-reactivity with rhMMP-3, -7, -8, -10, -12, or -13 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below
Immunohistochemistry	25-100 µg/mL	Immersion fixed paraffin-embedded sections of human ovarian and breast cancer tissues
Immunoprecipitation	25 µg/mL	Conditioned cell culture medium spiked with Recombinant Human MMP‑9 (Catalog # 911-MP), see our available Western blot detection antibodies
CyTOF-reported	Brodie, T.M. et al. (2018) Cytometry PartA. 93: 406. Ready to be labeled using establishedconjugation methods. No BSA or other carrier proteins that could interfere withconjugation.

Western Blot

Detection of Human MMP‑9 by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with 5 ng/mL PMA for 24 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MMP‑9 Monoclonal Antibody (Catalog # MAB911) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP‑9 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Related Product & Information

Long Name	Matrix Metalloproteinase 9
Background	MMP-9
background_content	Background: MMP-9 Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (Gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain.

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Citations

1. Diverse matrix metalloproteinase functions regulate cancer amoeboid migration.
   Authors: Orgaz J, Pandya P, Dalmeida R, Karagiannis P, Sanchez-Laorden B, Viros A, Albrengues J, Nestle F, Ridley A, Gaggioli C, Marais R, Karagiannis S, Sanz-Moreno V
   Nat Commun, 2014;5(0):4255.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. High matrix metalloproteinase production correlates with immune activation and leukocyte migration in leprosy reactional lesions.
   Authors: Teles RM, Teles RB, Amadeu TP, Moura DF, Mendonca-Lima L, Ferreira H, Santos IM, Nery JA, Sarno EN, Sampaio EP
   Infect. Immun., 2010;78(3):1012-21.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen


3. The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy.
   Authors: Li MQ, Hou XF, Shao J, Tang CL, Li DJ
   Biol. Reprod., 2010;82(5):968-79.
   Species: Human
   Sample Type: Whole Cells
   Application: Cell-based ELISA


4. Matrix metalloproteinase activity in pediatric acute lung injury.
   Authors: Kong MY, Gaggar A, Li Y
   Int J Med Sci, 2009;6(1):9-17.
   Species: Human
   Sample Type: Tissue Secretion
   Application: WB


5. Mediators of glioblastoma resistance and invasion during antivascular endothelial growth factor therapy.
   Authors: Lucio-Eterovic AK, Piao Y, de Groot JF
   Clin. Cancer Res., 2009;15(14):4589-99.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: WB


6. Long-term weight loss decreases the nontraditional cardiovascular risk factors interleukin-18 and matrix metalloproteinase-9 in obese subjects.
   Authors: Madsen EL, Bruun JM, Skogstrand K, Hougaard DM, Christiansen T, Richelsen B
   Metab. Clin. Exp., 2009;58(7):946-53.
   Species: Human
   Sample Type: Serum
   Application: Luminex Assay Development


7. Expression pattern and circulating levels of endostatin in patients with pancreas cancer.
   Authors: Ohlund D, Ardnor B, Oman M, Naredi P, Sund M
   Int. J. Cancer, 2008;122(12):2805-10.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen


8. Neutrophil gelatinase-associated lipocalin is expressed in osteoarthritis and forms a complex with matrix metalloproteinase 9.
   Authors: Gupta K, Shukla M, Cowland JB, Malemud CJ, Haqqi TM
   Arthritis Rheum., 2007;56(10):3326-35.
   Species: Human
   Sample Type: Synovial Fluid
   Application: IP


9. IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
   Authors: Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Del Vecchio Blanco G, Paoluzi OA, Caprioli F, Andrei F, Stolfi C, Romano M, Ricci V, MacDonald TT, Pallone F, Monteleone G
   J. Immunol., 2007;178(9):5957-65.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: WB


10. Hypoxia-induced mediators of stem/progenitor cell trafficking are increased in children with hemangioma.
    Authors: Kleinman ME, Greives MR, Churgin SS, Blechman KM, Chang EI, Ceradini DJ, Tepper OM, Gurtner GC
    Arterioscler. Thromb. Vasc. Biol., 2007;27(12):2664-70.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded

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