抗Human IL-10 R beta抗体(Anti-Human IL-10 R beta antibody)

• 2025/12/01

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  [RSD]厳選 抗体＆リコンビナントタンパク質 30％OFFキャンペーン[～2026/02/27] NEW

  期間：2025/12/01 ～2026/02/27

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  R&D Systems, Inc.（RSD）

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human IL-10 R beta Met20-Ser220Accession # Q08334
Source	Monoclonal Mouse IgG1 Clone # 90220
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL-10 R beta  in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑10 R alpha, rhIFN-gamma  RI, and rhIFN-gamma  R2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human IL‑10 R beta Fc Chimera (Catalog # 874-RB)
Flow Cytometry	0.25 µg/106 cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Neutralization	Measured by its ability to neutralize IL‑10 R beta -mediated IL‑10 response in LPS-activated human peripheral blood mononuclear cells (PBMC). Ralph, P. et al. (1991) J. Immunology 148:808. The Neutralization Dose (ND50) is typically 0.5-2 µg/mL in the presence of 0.25 ng/mL Recombinant Human IL‑10 and 0.25 ng/mL LPS.

Neutralization
	IL‑10 Inhibition of IL‑1 beta secre­tion and Neutralization by Human IL‑10 R beta  Antibody. Recombinant Human IL‑10 (Catalog # 217-IL) inhibits IL‑1 beta secretion in LPS-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line), as measured by the Human IL‑1 beta /IL‑1F2 Quantikine ELISA Kit (Catalog # DLB50). IL‑1 beta secre­tion inhibited by Recombinant Human IL‑10 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑10 R beta Monoclonal Antibody (Catalog # MAB874). The ND50 is typically 0.5-2 µg/mL in the presence of LPS (0.25 ng/mL).
Flow Cytometry
	Detection of IL‑10 R beta in Human PBMC Monocytes by Flow Cytometry Human PBMC monocytes were stained with Mouse Anti-Human IL‑10 R beta Monoclonal Antibody (Catalog # MAB874, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Long Name	Interleukin 10 Receptor beta
Entrez Gene IDs	3588 (Human); 16155 (Mouse)
Background	IL-10 R beta
background_content	Background: IL-10 R beta IL-10 R beta, also known as IL-10 R2, mediates its biological activities via binding to a heteromeric receptor complex consisting of two distinct type II cytokine receptor subunits, the ligand binding IL-10 R alpha and the IL-10 R beta which does not bind IL-10 by itself but is required for signal transduction. The cDNA for human IL-10 R beta encodes a 325 amino acid (aa) type I transmembrane precursor protein with a 20 aa signal sequence, a 200 aa extracellular region, a 29 aa transmembrane segment and a 76 aa cytoplasmic domain. Within the extracellular region, there are two 100 aa subdomains that resemble the constant region of immunoglobulins. This structural motif is responsible for the alternative designation of IL-10 R beta as CRF2-4 (the 4th member of the cytokine receptor family class II/2). Human and mouse IL-10 R beta share approximately 69% aa sequence identity. Binding of the non-covalent IL-10 dimer to two IL-10 R alpha chains recruits two IL‑10 R beta chains resulting in the activation and phosphorylation of the signaling cascade involving JAK1, TYK2, and STAT3. IL-10 R beta  is expressed ubiquitously. IL‑10 R beta is also a component of the IL-22 receptor complex consisting of the IL-10 R beta chain and IL-22 R, another type II cytokine receptor family member.

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Citations

1. IFN-?4 Attenuates Antiviral Responses by Enhancing Negative Regulation of IFN Signaling
   Authors: AA Obajemu, N Rao, KA Dilley, JM Vargas, F Sheikh, RP Donnelly, RS Shabman, EG Meissner, L Prokunina-, OO Onabajo
   J. Immunol., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. Immune Cell Profiling of IFN-? Response Shows pDCs Express Highest Level of IFN-?R1 and Are Directly Responsive Via the JAK-STAT Pathway
   J Interferon Cytokine Res, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. IL22/IL-22R pathway induces cell survival in human glioblastoma cells.
   Authors: Akil H, Abbaci A, Lalloue F, Bessette B, Costes L, Domballe L, Charreau S, Guilloteau K, Karayan-Tapon L, Bernard F, Morel F, Jauberteau M, Lecron J
   PLoS ONE, 2015;10(3):e0119872.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Differential signaling of cmvIL-10 through common variants of the IL-10 receptor 1.
   Authors: Gruber SG, Gloria Luciani M, Grundtner P, Zdanov A, Gasche C
   Eur. J. Immunol., 2008;38(12):3365-75.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Immunomodulatory properties of a viral homolog of human interleukin-10 expressed by human cytomegalovirus during the latent phase of infection.
   Authors: Jenkins C, Garcia W, Godwin MJ, Spencer JV, Stern JL, Abendroth A, Slobedman B
   J. Virol., 2008;82(7):3736-50.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut

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