抗Human IL-18 R alpha/IL-1 R5抗体(Anti-Human IL-18 R alpha/IL-1 R5 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human IL‑18 R alpha /IL-1 R5Glu20-Arg329Accession # Q13478
Source	Monoclonal Mouse IgG1 Clone # 70625
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects Recombinant Human IL-18 R alpha /IL-1 R5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-1 R3, rhIL-1 R4, rhIL-1 R6, rhIL-1 R7, rhIL-1 R8, rhIL-1 R9, rhSIGIRR or recombinant mouse IL-18 R alpha is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/106 cells	Human peripheral blood mononuclear cells treated with PHA and Recombinant Human IL‑2 (Catalog # 202-IL)
Immunohistochemistry	8-25 µg/mL	Immersion fixed paraffin-embedded sections of human skin
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below
Neutralization	Measured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in KG-1 human myeloid leukemia cells. The Neutralization Dose (ND50) is typically 0.03-0.1 µg/mL in the presence of 40 ng/mL Recombinant Human IL‑18/IL‑1F4 and 20 ng/mL Recombinant Human TNF-alpha.

Neutralization
	IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutrali­zation by IL‑18 R alpha /IL‑1 R5 Antibody. In the presence of Recombinant Human TNF‑ alpha (20 ng/mL, Catalog # 210-TA), Recombinant Human IL‑18/IL‑1F4 (Catalog # B003-5) stimulates IFN‑ gamma secretion in KG-1 human myeloid leukemia cells in a dose-dependent manner (orange line), as measured by the Human IFN‑ gamma Quantikine ELISA Kit (Catalog # DIF50). Under these conditions, IFN‑ gamma secretion elicited by Recombinant Human IL‑18/IL‑1F4 (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑18 R alpha /IL‑1 R5 Monoclonal Antibody (Catalog # MAB840). The ND50 is typically 0.03‑0.1 µg/mL.
Immunocytochemistry
	IL‑18 R alpha /IL‑1 R5 in Human PBMCs. IL‑18 R alpha /IL‑1 R5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Human IL‑18 R alpha /IL‑1 R5 Mono­clonal Antibody (Catalog # MAB840) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Long Name	Interleukin 18 Receptor alpha
Entrez Gene IDs	8809 (Human); 16182 (Mouse)
Background	IL-18 R alpha/IL-1 R5
background_content	Background: IL-18 R alpha/IL-1 R5 Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL‑18 R beta is required for high affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.

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Citations

1. Interleukin-37 expression and its potential role in oral leukoplakia and oral squamous cell carcinoma
   Sci Rep, 2016;6(0):26757.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


2. Interleukin 18 function in atherosclerosis is mediated by the interleukin 18 receptor and the Na-Cl co-transporter.
   Authors: Wang J, Sun C, Gerdes N, Liu C, Liao M, Liu J, Shi M, He A, Zhou Y, Sukhova G, Chen H, Cheng X, Kuzuya M, Murohara T, Zhang J, Cheng X, Jiang M, Shull G, Rogers S, Yang C, Ke Q, Jelen S, Bindels R, Ellison D, Jarolim P, Libby P, Shi G
   Nat Med, 2015;21(7):820-6.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen


3. Regulation of development of CD56 bright CD11c + NK-like cells with helper function by IL-18.
   Authors: Li W, Okuda A, Yamamoto H, Yamanishi K, Terada N, Yamanishi H, Tanaka Y, Okamura H
   PLoS ONE, 2013;8(12):e82586.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. IL-1 Family Members IL-18 and IL-33 Upregulate the Inflammatory Potential of Differentiated Human Th1 and Th2 Cultures.
   Authors: Blom L, Poulsen L
   J Immunol, 2012;189(9):4331-7.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis.
   Authors: Takei S, Hoshino T, Matsunaga K, Sakazaki Y, Sawada M, Oda H, Takenaka S, Imaoka H, Kinoshita T, Honda S, Ida H, Fukuda TA, Aizawa H
   Arthritis Res. Ther., 2011;13(2):R52.
   Species: Human
   Sample Type: Serum
   Application: WB


6. Glomerular accumulation of plasmacytoid dendritic cells in active lupus nephritis: role of interleukin-18.
   Authors: Tucci M, Quatraro C, Lombardi L, Pellegrino C, Dammacco F, Silvestris F
   Arthritis Rheum., 2008;58(1):251-62.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response.
   Authors: Rao DA, Tracey KJ, Pober JS
   J. Immunol., 2007;179(10):6536-46.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. CD molecules 2006--human cell differentiation molecules.
   Authors: Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X
   J. Immunol. Methods, 2007;319(1):1-5.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


9. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
   Authors: Ebert EC, Mehta V
   Cell. Immunol., 2006;241(1):7-13.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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