抗Human uPAR抗体(Anti-Human uPAR antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• References
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human uPARLeu23-Arg303Accession # Q03405
Source	Monoclonal Mouse IgG1 Clone # 62022
Purification	Protein A or G purified from ascites
Specificity	Detects human uPAR in direct ELISAs and Western blots. When used in a sandwich ELISA in combination with the biotinylated anti-human uPAR detection antibody (Catalog # BAF807), no significant cross-reactivity was observed with recombinant mouse uPAR.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human uPAR (Catalog # 807-UK) under non-reducing conditions only
Flow Cytometry	0.25 µg/106 cells	See below
Blockade of Receptor-ligand Interaction	In a functional ELISA, 0.5-1.5 µg/mL of this antibody will block 50% of the binding of 30 ng/mL of Recombinant Human uPAR (Catalog # 807-UK) to immobilized Recombinant Human u-Plasminogen Activator/Urokinase (Catalog # 1310-SE) coated at 500 ng/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Human uPAR Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human uPAR Antibody (Catalog #MAB807 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Human uPAR Biotinylated Antibody (Catalog #BAF807 )
ELISA Standard		Recombinant Human uPAR Protein (Catalog #807-UK )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Flow Cytometry	click image to view larger	Detection of uPAR in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human uPAR Monoclonal Antibody (Catalog # MAB807, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: uPAR	The urokinase-type Plasminogen Activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a single-chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity. The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. uPAR cDNA encodes a 335 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, five potential N-linked glycosylation sites and a C‑terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% aa sequence identity and the receptor-ligand interaction is strictly species-specific.

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References

Dear, A.E. and R.L. Medcalf (1988) Eur. J. Biochemistry 252:185.
Long Name:	Urokinase-type Plasminogen Activator Receptor
Entrez Gene IDs:	5329 (Human); 18793 (Mouse)
Alternate Names:	CD87 antigen; CD87; Monocyte activation antigen Mo3; plasminogen activator, urokinase receptor; PLAUR; uPAR; U-PAR; UPARurokinase plasminogen activator surface receptor; u-plasminogen activator receptor form 2; URKRMO3

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Related Research Areas

Cancer Biomarkers

Coagulation

Complement Pathway

Extracellular Matrix Remodeling and Cell Migration During EMT

HIF Transcription Factors

Plasmin/Plasminogen and Kallikrein/Kinin Systems

Serine Proteases and Regulators

Negative regulation of urokinase receptor activity by a GPI-specific phospholipase C in breast cancer cells Authors: M van Veen, E Matas-Rico, K van de Wet, D Leyton-Pui, KM Kedziora, V De Lorenzi, Y Stijf-Bult, B van den Br, K Jalink, N Sidenius, A Perrakis, WH Moolenaar Elife, 2017;6(0):. Species: Human Sample Type: Whole Cells Application: Flow

Flow Cytometry

Detection of uPAR in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human uPAR Monoclonal Antibody (Catalog # MAB807, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Long Name	Urokinase-type Plasminogen Activator Receptor
Entrez Gene IDs	5329 (Human); 18793 (Mouse)
Background	uPAR
background_content	Background: uPAR The urokinase-type Plasminogen Activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as asingle-chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity. The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. uPAR cDNA encodes a 335 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, five potential N-linked glycosylation sites and aC‑terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% aa sequence identity and the receptor-ligand interaction is strictly species-specific.

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Citations

1. Negative regulation of urokinase receptor activity by a GPI-specific phospholipase C in breast cancer cells
   Authors: M van Veen, E Matas-Rico, K van de Wet, D Leyton-Pui, KM Kedziora, V De Lorenzi, Y Stijf-Bult, B van den Br, K Jalink, N Sidenius, A Perrakis, WH Moolenaar
   Elife, 2017;6(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. The periodontal pathogen Porphyromonas gingivalis preferentially interacts with oral epithelial cells in S phase of the cell cycle
   Authors: FB Al-Taweel, CW Douglas, SA Whawell
   Infect Immun, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Soluble Urokinase Receptor Is Released Selectively by Glioblastoma Cells That Express Epidermal Growth Factor Receptor Variant III and Promotes Tumor Cell Migration and Invasion.
   Authors: Gilder A, Jones K, Hu J, Wang L, Chen C, Carter B, Gonias S
   J Biol Chem, 2015;290(24):14798-809.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. Intracellular modifiers of integrin alpha 6p production in aggressive prostate and breast cancer cell lines.
   Authors: Kacsinta A, Rubenstein C, Sroka I, Pawar S, Gard J, Nagle R, Cress A
   Biochem Biophys Res Commun, 2014;454(2):335-40.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Plasmin cleavage of von Willebrand factor as an emergency bypass for ADAMTS13 deficiency in thrombotic microangiopathy.
   Authors: Tersteeg C, de Maat S, De Meyer S, Smeets M, Barendrecht A, Roest M, Pasterkamp G, Fijnheer R, Vanhoorelbeke K, de Groot P, Maas C
   Circulation, 2014;129(12):1320-31.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay


6. Urinary soluble urokinase receptor levels are elevated and pathogenic in patients with primary focal segmental glomerulosclerosis.
   Authors: Huang J, Liu G, Zhang Y, Cui Z, Wang F, Liu X, Chu R, Zhao M
   BMC Med, 2014;12(0):81.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. Activation of the plasma kallikrein-kinin system on human lung epithelial cells.
   Authors: Vergiliana JF, Asokananthan N, Stewart GA
   Biol. Chem., 2010;391(9):1067-77.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. AP-1-controlled hepatocyte growth factor activation promotes keratinocyte migration via CEACAM1 and urokinase plasminogen activator/urokinase plasminogen receptor.
   Authors: Schnickmann S, Camacho-Trullio D, Bissinger M, Eils R, Angel P, Schirmacher P, Szabowski A, Breuhahn K
   J. Invest. Dermatol., 2009;129(5):1140-8.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


9. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
   Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
   Transplantation, 2009;87(12):1814-20.
   Species: Human
   Sample Type: Urine
   Application: Antibody Array Development


10. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008;7(6):2406-14.
    Species: Human
    Sample Type: Serum
    Application: ELISA Microarray Development

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