抗Mouse IL-17抗体(Anti-Mouse IL-17 antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• References
• Related Research Areas
• Summary
• Summary
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse IL‑17Thr22-Ala158Accession # Q62386
Source	Monoclonal Rat IgG2A Clone # 50101
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse IL-17 in ELISAs and Western blots. In direct ELISAs, 100% reactivity with recombinant mouse (rm) IL-17A/IL-17F heterodimer, 50% cross-reactivity with recombinant human IL-17, and 25% cross-reactivity with recombinant canine IL-17 is observed. No cross-reactivity with rmIL-17B, rmIL-17C, rmIL-17D, rmIL-17E, or rmIL-17F is observed. In Western blots, cross-reactivity with recombinant rat IL-17A is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Mouse IL-17 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Mouse IL‑17/IL‑17A Antibody (Catalog #MAB721 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse IL‑17 Biotinylated Antibody (Catalog #BAF421 )
ELISA Standard		Recombinant Mouse IL-17A Protein (Catalog #421-ML )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Western Blot	click image to view larger	Detection of Recombinant Mouse and Rat IL‑17/IL‑17A by Western Blot. Western blot shows 25 ng of Recombinant Mouse IL‑17/IL‑17A (Catalog # 421-ML), Recombinant Human IL‑17/IL‑17A (Catalog # 317-ILB), Recombinant Rat IL‑17/IL‑17A (Catalog # 8410-IL), and Recombinant Mouse IL‑17F (Catalog # 2057-IL). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IL‑17/IL‑17A Monoclonal Antibody (Catalog # MAB721) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL‑17/IL‑17A at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-17/IL-17A	Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield the 137 aa residue mature  IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, mouse IL-17 shows 57% and 87% sequence identity with herpes virus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has been cloned. While the expression of IL-17 mRNA is restricted to activated alpha beta TCR+CD4-CD8- T cells, the expression of mouse IL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-kappa B and costimulation of T cell proliferation.

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References

Kennedy, J. et al. (1996) J. Interferon Cytokine Res. 16:611.
Yao, Z. et al. (1995) J. Immunol. 155:5483.
Yao, Z. et al. (1995) Immunity 3:811.
Rouvier, E. et al. (1993) J. Immunol. 150:5445.
Long Name:	Interleukin 17
Entrez Gene IDs:	3605 (Human); 16171 (Mouse); 301289 (Rat); 481837 (Canine)
Alternate Names:	CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

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Related Research Areas

Show

B Cell Chemotaxis, Migration, and Adhesion
Cytokines and Receptors in Tfh Cell Differentiation and Function
gamma delta T Cells
Helper T (Th) Cells
IL-17 Family
LTi Cells
Macrophage Activation Markers
Molecules Secreted by VSMC
Multiple Sclerosis and Myelin Related Molecules
Natural Killer T (NKT) Cells
NCR-ILC3
Neuroinflammation
T Cell Cytokine Signaling
Th17 Cells
The Major Histocompatibility Complex Class III Haplotype Ltab-Ncr3 Regulates Adjuvant-Induced but Not Antigen-Induced Autoimmunity Authors: AC Yau, J Tuncel, R Holmdahl Am. J. Pathol, 2017;0(0):. Species: Rat Sample Type: Tissue Homogenates Application: ELISA Capture
Images	Ratings	Applications	Species	Reviewed By	Date	Details
Enlarge	Excellent		ELISA	Mouse	Anonymous	11/27/2017			Image Details	ELISA: Mouse IL-17/IL-17A Antibody [MAB721].

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Summary

Show

Application	ELISA
Sample Tested	Serum and Plasma
Species	Mouse
Other Experimental Details	This antibody was used to build and ELISA. It was used as the capture with BAF421 as the detection antibody.
	Excellent		Affinity Purification	Drosophila	Anonymous	08/11/2017

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Summary

Application	Affinity Purification
Sample Tested	Blood erythrocytes
Species	Drosophila

Western Blot

Detection of Recombinant Mouse and Rat IL‑17/IL‑17A by Western Blot. Western blot shows 25 ng of Recombinant Mouse IL‑17/IL‑17A (Catalog # 421-ML), Recombinant Human IL‑17/IL‑17A (Catalog # 317-ILB), Recombinant Rat IL‑17/IL‑17A (Catalog # 8410-IL), and Recombinant Mouse IL‑17F (Catalog # 2057-IL). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IL‑17/IL‑17A Monoclonal Antibody (Catalog # MAB721) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL‑17/IL‑17A at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

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Related Product & Information

Background

IL-17/IL-17A

background_content

Background: IL-17/IL-17A Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield the 137 aa residue mature  IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, mouse IL-17 shows 57% and 87% sequence identity with herpes virus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has been cloned. While the expression of IL-17 mRNA is restricted to activated alpha beta TCR+CD4-CD8- T cells, the expression of mouse IL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-kappa B and costimulation of T cell proliferation.

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Citations

1. The Major Histocompatibility Complex Class III Haplotype Ltab-Ncr3 Regulates Adjuvant-Induced but Not Antigen-Induced Autoimmunity
   Authors: AC Yau, J Tuncel, R Holmdahl
   Am. J. Pathol, 2017;0(0):.
   Species: Rat
   Sample Type: Tissue Homogenates
   Application: ELISA Capture


2. Interferon-gamma regulates inflammatory cell death by targeting necroptosis in experimental autoimmune arthritis
   Authors: SH Lee, JY Kwon, SY Kim, K Jung, ML Cho
   Sci Rep, 2017;7(1):10133.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


3. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
   Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
   Cell. Physiol. Biochem., 2017;42(5):1961-1972.
   Species: Mouse
   Sample Type: Serum
   Application: ELISA Capture


4. Targeting dendritic cells to accelerate T-cell activation overcomes a bottleneck in tuberculosis vaccine efficacy
   Nat Commun, 2016;7(0):13894.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ELISpot Development


5. MHC class II alleles associated with Th1 rather than Th17 type immunity drive the onset of early arthritis in a rat model of rheumatoid arthritis
   Eur. J. Immunol, 2016;0(0):.
   Species: Rat
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


6. Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis
   Authors: Jung-Yeon Lim
   Sci Rep, 2016;6(0):26851.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


7. Immune requirements for protective Th17 recall responses to Mycobacterium tuberculosis challenge.
   Authors: Monin L, Griffiths K, Slight S, Lin Y, Rangel-Moreno J, Khader S
   Mucosal Immunol, 2015;8(5):1099-109.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ELISpot Development


8. CD8(+) cells regulate the T helper-17 response in an experimental murine model of Sjogren syndrome.
   Authors: Zhang X, Schaumburg C, Coursey T, Siemasko K, Volpe E, Gandhi N, Li D, Niederkorn J, Stern M, Pflugfelder S, de Paiva C
   Mucosal Immunol, 2014;7(2):417-27.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ELISA Capture


9. Interferon gamma suppresses collagen-induced arthritis by regulation of Th17 through the induction of indoleamine-2,3-deoxygenase.
   Authors: Lee J, Lee J, Park M, Lim M, Park E, Kim E, Yang E, Lee S, Jhun J, Park S, Kim H, Cho M
   PLoS ONE, 2013;8(4):e60900.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


10. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Type: Plasma
    Application: Luminex Assay Development

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