抗Human TNF RI/TNFRSF1A抗体(Anti-Human TNF RI/TNFRSF1A antibody)

• 価格
• Product Details
• Applications and Data
• Data Example
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human TNF RI/TNFRSF1AExtracellular domain
Source	Monoclonal Mouse IgG1 Clone # 16805
Purification	Protein A or G purified from ascites
Specificity	Detects human TNF RI/TNFRSF1A in ELISAs and Western blots. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) TGF-alpha, hTGF-beta 1, porcine (p) TGF-beta 1, rhTGF-beta 1, pTGF-beta 1.2, pTGF-beta 2, recombinant chicken TGF-beta 3, rhTGF-beta 3, recombinant amphibian TGF-beta 5, rhTNF-beta, or rhTNF RII is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human TNF RI/TNFRSF1A (Catalog # 636-R1) under non-reducing conditions only
Human TNF RI/TNFRSF1A Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human TNF RI/TNFRSF1A Antibody (Catalog #MAB625 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Human TNF RI/TNFRSF1A Biotinylated Antibody (Catalog #BAF225 )
ELISA Standard		Recombinant Human sTNF RI/TNFRSF1A Protein (Catalog #636-R1 )
Neutralization	Measured by its ability to neutralize TNF RI/TNFRSF1A-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 1-6 µg/mL in the presence of 0.3 μg/mL Recombinant Human TNF RI/TNFRSF1A, 0.25 ng/mL Recombinant Human TNF‑ alpha, and 1 µg/mL actinomycin D.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Example

1 month, 2 to 8 °C under sterile conditions after reconstitution.6 months, -20 to -70 °C under sterile conditions after reconstitution.

Neutralization	click image to view larger	TNF RI/TNFRSF1A Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RI/TNFRSF1A Antibody. Recombinant Human TNF RI/TNFRSF1A (Catalog # 636‑R1) inhibits Recombinant Human TNF‑ alpha (Catalog # 210‑TA) induced cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human TNF‑ alpha (0.25 ng/mL) activity elicited by Recombinant Human TNF RI/TNFRSF1A (0.3 μg/mL) is neutralized (green line) by increasing concentrations of Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB625). The ND50 is typically 1-6 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
Background: TNF RI/TNFRSF1A	TNF RI occurs both in membrane bound and soluble forms and functions as a receptor for TNF-alpha and TNF-beta. In the superfamily nomenclature, it is designated TNFRSF1A.
Long Name:	Tumor Necrosis Factor Receptor I
Entrez Gene IDs:	7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Alternate Names:	CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor
Related Research Areas	Adipocytokines Cell Structure and Signaling Molecules Cytokine and Growth Factor Receptors on VSMC Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Diabetes Diabetic Peripheral Neuropathy EMT Induction Endothelial Cell Markers Neuroinflammation Obesity Platelet Cytokine and Growth Factor Receptors Regulation of Apoptosis by TNF Superfamily Members T Cell Cytokine Signaling TNF Superfamily Receptors

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Related Product & Information

Long Name	Tumor Necrosis Factor Receptor I
Entrez Gene IDs	7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Background	TNF RI/TNFRSF1A
background_content	Background: TNF RI/TNFRSF1A TNF RI occurs both in membrane bound and soluble forms and functions as a receptor for TNF-alpha and TNF-beta. In the superfamily nomenclature, it is designated TNFRSF1A.

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Citations

1. CD16 is indispensable for antibody-dependent cellular cytotoxicity by human monocytes
   Sci Rep, 2016;6(0):34310.
   Species: Human
   Sample Type:
   


2. Generation and characterization of small single domain antibodies inhibiting human tumor necrosis factor receptor 1.
   Authors: Steeland S, Puimege L, Vandenbroucke R, Van Hauwermeiren F, Haustraete J, Devoogdt N, Hulpiau P, Leroux-Roels G, Laukens D, Meuleman P, De Vos M, Libert C
   J Biol Chem, 2015;290(7):4022-37.
   Species: Human
   Sample Type: Complex Sample Type
   Application: ELISA Capture


3. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
   Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
   J. Immunol. Methods, 2012;378(1):72-80.
   Species: Human
   Sample Type: Serum
   Application: ELISA Development


4. CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation.
   Authors: Denoyer A, Godefroy D, Celerier I, Frugier J, Riancho L, Baudouin F, Rostene W, Baudouin C
   Mucosal Immunol, 2012;5(0):702.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. IRF5 is required for late-phase TNF secretion by human dendritic cells.
   Authors: Krausgruber T, Saliba D, Ryzhakov G
   Blood, 2010;115(22):4421-30.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
   Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
   Transplantation, 2009;87(12):1814-20.
   Species: Human
   Sample Type: Urine
   Application: Antibody Array Development


7. Simultaneous induction of apoptotic and survival signaling pathways in macrophage-like THP-1 cells by Shiga toxin 1.
   Authors: Lee SY, Cherla RP, Tesh VL
   Infect. Immun., 2007;75(3):1291-302.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay


8. Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production.
   Authors: Emsley HC, Smith CJ, Gavin CM
   BMC Neurol, 2007;7(0):5.
   Species: Human
   Sample Type: Plasma
   Application: ELISA Development


9. LIGHT (TNFSF14), a novel mediator of bone resorption, is elevated in rheumatoid arthritis.
   Authors: Edwards JR, Sun SG, Locklin R, Shipman CM, Adamopoulos IE, Athanasou NA, Sabokbar A
   Arthritis Rheum., 2006;54(5):1451-62.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


10. Synergistic induction of apoptosis in breast cancer cells by cotreatment with butyrate and TNF-alpha, TRAIL, or anti-Fas agonist antibody involves enhancement of death receptors' signaling and requires P21(waf1).
    Authors: Chopin V, Slomianny C, Hondermarck H, Le Bourhis X
    Exp. Cell Res., 2004;298(2):560-73.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

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