抗Mouse CX3CL1/Fractalkine抗体(Anti-Mouse CX3CL1/Fractalkine antibody)
掲載日情報:2019/10/21 現在Webページ番号:28457
Mouse CX3CL1/Fractalkineに対する抗体(Anti-Mouse CX3CL1/Fractalkine )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 :2025年04月26日 20時35分現在]
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Anti-Mouse CX3CL1/Fractalkine MAb (Clone 126315) |
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Anti-Mouse CX3CL1/Fractalkine MAb (Clone 126315) |
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Anti-Mouse CX3CL1/Fractalkine MAb (Clone 126315) |
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[在庫・価格 :2025年04月26日 20時35分現在]
Anti-Mouse CX3CL1/Fractalkine MAb (Clone 126315)
文献数: 0
- 商品コード:MAB571-100
- メーカー:RSD
- 包装:100μg
- 価格:¥101,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | 別名:ABCD-3 クローン:126315 Genbank No: 6376 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | 免疫動物 | Rat | |||||
交差性 | Mouse | 適用 | ELISA,Neutralising,Western Blot | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | M1/M2 Macrophage Activation Marker |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
Anti-Mouse CX3CL1/Fractalkine MAb (Clone 126315)
文献数: 0
- 商品コード:MAB571-500
- メーカー:RSD
- 包装:500μg
- 価格:¥232,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | マッチドペア:Mouse CX3CL1/Fractalkine サンドイッチELISAの補足用抗体として利用可能,検出用抗体として#BAF472,スタンダードとして#472-FF-025を用いる。 別名:ABCD-3 クローン:126315 Genbank No: 6376 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | 免疫動物 | Rat | |||||
交差性 | Mouse | 適用 | ELISA,Neutralising,Western Blot | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | M1/M2 Macrophage Activation Marker |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
Anti-Mouse CX3CL1/Fractalkine MAb (Clone 126315)
文献数: 0
- 商品コード:MAB571-SP
- メーカー:RSD
- 包装:25μg
- 価格:¥30,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | ※受注発注品。形状:溶液または凍結乾燥 別名:ABCD-3 クローン:126315 Genbank No: 6376 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | 免疫動物 | Rat | |||||
交差性 | Mouse | 適用 | ELISA,Neutralising,Western Blot | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | M1/M2 Macrophage Activation Marker 使いっきり抗体 |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
Species Reactivity | Mouse |
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Label | Unconjugated |
Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse CX3CL1/Fractalkine, S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse CX3CL1/Fractalkine, and E. coli-derived recombinant mouse CX3CL1/Fractalkine |
Source | Monoclonal Rat IgG2A Clone # 126315 |
Purification | Protein A or G purified from hybridoma culture supernatant |
Specificity | Detects mouse CX3CL1/Fractalkine in Western blots. In direct ELISAs, this antibody shows 100% cross-reactivity with the chemokine domain of recombinant human (rh) CX3CL1, 13% cross-reactivity with rhCX3CL1, and 50% cross-reactivity with recombinant rat CX3CL1. |
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Applications and Data
Recommended Concentration | Sample | |
Western Blot | 1 µg/mL | Recombinant Mouse CX3CL1/Fractalkine Full Length (Catalog # 472-FF) under non-reducing conditions only |
Mouse CX3CL1/Fractalkine Sandwich Immunoassay | Reagent | |
ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Mouse CX3CL1/Fractalkine Chemokine Domain Antibody (Catalog #MAB571 ) |
ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody (Catalog #BAF472 ) |
ELISA Standard | Recombinant Mouse CX3CL1/Fractalkine (Full Length) Protein (Catalog #472-FF ) | |
Neutralization | Measured by its ability to neutralize CX3CL1/Fractalkine-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CX3CR1. The Neutralization Dose (ND50) is typically 0.75-3.5 µg/mL in the presence of 30 ng/mL Recombinant Mouse CX3CL1/Fractalkine aa 25‑105. | |
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. |
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Data Example
Neutralization | ![]() click image to view larger | Chemotaxis Induced by CX3CL1/Fractalkine and Neutralization by Mouse CX3CL1/Fractalkine Antibody. Recombinant Mouse CX3CL1/Fractalkine (Catalog # 571-MF) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CX3CL1/Fractalkine (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB571). The ND50 is typically 0.75‑3.5 µg/mL. |
Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | Background: CX3CL1/Fractalkine | CX3CL1, also known as Fractalkine, is a type I membrane protein in which a chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site. |
Entrez Gene IDs: | 6376 (Human); 20312 (Mouse); 89808 (Rat) | |
Alternate Names: | ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1 | |
Related Research Areas | Cancer Stem Cell Markers Chemokines and Receptors Chemokines and Receptors in Allergy and Asthma Chemokines and Receptors in Angiogenesis CX3C Chemokines and Receptors >Macrophage Activation Markers Molecules Secreted by VSMC Neuroinflammation Renal Markers Special Glycoproteins Uptake of Amyloid beta by Macrophages |
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Related Product & Information
Entrez Gene IDs | 6376 (Human); 20312 (Mouse); 89808 (Rat) |
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Background | CX3CL1/Fractalkine |
background_content | Background: CX3CL1/Fractalkine CX3CL1, also known as Fractalkine, is a type I membrane protein in which a chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site. |
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Citations
- p16Ink4a and p21Cip1/Waf1 promote tumour growth by enhancing myeloid-derived suppressor cells chemotaxis
Authors: A Okuma, A Hanyu, S Watanabe, E Hara
Nat Commun, 2017;8(1):2050.
Species: Mouse
Sample Type: In Vivo
Application: In Vivo - PGI2 Controls Pulmonary NK Cells That Prevent Airway Sensitization to House Dust Mite Allergen
Authors: Bryan Simons
J. Immunol, 2016;0(0):.
Species: Mouse
Sample Type:
Application: In vivo - Extracellular adenosine signaling induces CX3CL1 expression in the brain to promote experimental autoimmune encephalomyelitis.
J Neuroinflammation, 2012;9(0):193.
Species: Mouse
Sample Type: In Vivo
Application: In vivo - Gene therapy with CX3CL1/Fractalkine induces antitumor immunity to regress effectively mouse hepatocellular carcinoma.
Authors: Tang L, Hu HD, Hu P, Lan YH, Peng ML, Chen M, Ren H
Gene Ther., 2007;14(16):1226-34.
Species: Mouse
Sample Type: Whole Cells
Application: ICC
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