抗Human/Mouse IL-12/IL-35 p35抗体(Anti-Human/Mouse IL-12/IL-35 p35 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35Arg23-Ser219Accession # P29459
Source	Monoclonal Mouse IgG1 Clone # 27537
Purification	Protein A or G purified from ascites
Specificity	Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human IL-12 (Catalog # 219-IL)
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	2.5 µg/106 cells	Human peripheral blood mononuclear cells or mouse splenocytes treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin

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Related Product & Information

Background

IL-12/IL-35 p35

background_content

Background: IL-12/IL-35 p35 Interleukin 12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is comprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3+ regulatory T cells and may function as an inhibitory cytokine to suppress T cell proliferation (2). Human FoxP3+ Tregs do not constitutively express IL-35 (3), but expression may be induced by activated dendritic cells (4).

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Citations

1. Dendritic Cell-Secreted Cytotoxic T-Lymphocyte-Associated Protein-4 Regulates the T-cell Response by Downmodulating Bystander Surface B7
   Authors: MM Halpert, V Konduri, D Liang, Y Chen, JB Wing, S Paust, JM Levitt, WK Decker
   Stem Cells Dev., 2016;25(10):774-87.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: WB


2. Gene expression and antiviral activity of interleukin-35 in response to influenza A virus infection
   Authors: Li Wang
   J Biol Chem, 2016;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Ex vivo-expanded but not in vitro-induced human regulatory T cells are candidates for cell therapy in autoimmune diseases thanks to stable demethylation of the FOXP3 regulatory T cell-specific demethylated region.
   Authors: Rossetti M, Spreafico R, Saidin S, Chua C, Moshref M, Leong J, Tan Y, Thumboo J, van Loosdregt J, Albani S
   J Immunol, 2015;194(1):113-24.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. Dendritic cell SIRT1-HIF1alpha axis programs the differentiation of CD4+ T cells through IL-12 and TGF-beta1.
   Authors: Liu G, Bi Y, Xue L, Zhang Y, Yang H, Chen X, Lu Y, Zhang Z, Liu H, Wang X, Wang R, Chu Y, Yang R
   Proc Natl Acad Sci U S A, 2015;112(9):E957-65.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: Bioassay


5. Deltex1 antagonizes HIF-1alpha and sustains the stability of regulatory T cells in vivo.
   Authors: Hsiao H, Hsu T, Liu W, Hsieh W, Chou T, Wu Y, Jiang S, Lai M
   Nat Commun, 2015;6(0):6353.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


6. Oral Escherichia coli colonization factor antigen I fimbriae ameliorate arthritis via IL-35, not IL-27.
   Authors: Kochetkova I, Thornburg T, Callis G, Holderness K, Maddaloni M, Pascual D
   J Immunol, 2014;192(2):804-16.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


7. Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade.
   Authors: Olson B, Jankowska-Gan E, Becker J, Vignali D, Burlingham W, McNeel D
   J Immunol, 2012;189(12):5590-601.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. Human rhinoviruses induce IL-35-producing Treg via induction of B7-H1 (CD274) and sialoadhesin (CD169) on DC.
   Authors: Jindra C
   Eur J Immunol, 2010;40(2):321-9.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: IP


9. Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma.
   Authors: Maneechotesuwan K, Wamanuttajinda V, Kasetsinsombat K, Huabprasert S, Yaikwawong M, Barnes PJ, Wongkajornsilp A
   J. Allergy Clin. Immunol., 2009;123(1):239-48.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


10. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
    Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
    Eur. J. Immunol., 2008;38(4):1012-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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