抗Mouse NKG2D抗体(Anti-Mouse NKG2D antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse NKG2DPhe94-Val232Accession # O54709
Source	Monoclonal Rat IgG2B Clone # 191004
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse NKG2D in direct ELISAs and Western blots. Does not cross-react with recombinant human NKG2D.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Mouse NKG2D/CD314 Fc Chimera (Catalog # 139-NK) under non-reducing conditions only
Flow Cytometry	2.5 µg/106 cells	NK1.1+ mouse splenic NK cells
Blockade of Receptor-ligand Interaction	In a functional ELISA, 0.05-0.15 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of biotinylated Recombinant Mouse Rae-1 gamma Fc Chimera to immobilized Recombinant Mouse NKG2D Fc Chimera (Catalog # 139‑NK) coated at 4 µg/mL (100 µL/well). At 2 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

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Related Product & Information

Long Name	Natural Killer G2D
Entrez Gene IDs	22914 (Human); 27007 (Mouse)
Background	NKG2D/CD314
background_content	Background: NKG2D/CD314 NKG2D is a type II transmembrane protein with an extracellular C-type lectin-like domain. It occurs as a disulfide-linked homodimer that associates with the transmembrane DAP10 (DNAX-activator protein 10) adapter protein to deliver an activating signal. This protein shares approximately 25% amino acid sequence identity with a number of other type II lectin-like proteins that are encoded by genes within the natural killer complex on mouse chromosome 6. NKG2D is expressed on NK cells, where it functions as an activating receptor to trigger cytolytic activity and cytokine secretion, and on some T cell subsets, where it acts as a co‑stimulatory receptor complementing T cell receptor signaling. Several ligands have now been identified for mouse NKG2D including H60 and Rae 1 alpha, beta, gamma, δ, and epsilon. All of these ligands are cell-surface proteins distantly related to MHC class I. However, they do not bind peptide or associate with beta 2-microglobulin. Ligand expression is up-regulated in many transformed cell lines and also during conditions of stress such as heat shock or viral infection. In vivo, tumor models demonstrate that NKG2D functions in anti-tumor surveillance (1-5).

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Citations

1. NKG2D ligand RAE1? induces generation and enhances the inhibitor function of myeloid-derived suppressor cells in mice
   Authors: L Qian, Y Liu, S Wang, W Gong, X Jia, L Liu, F Ye, J Ding, Y Xu, Y Fu, F Tian
   J. Cell. Mol. Med, 2017;0(0):.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


2. NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity.
   Authors: Nielsen M, Dyring-Andersen B, Schmidt J, Witherden D, Lovato P, Woetmann A, Odum N, Poulsen S, Havran W, Geisler C, Bonefeld C
   J Invest Dermatol, 2015;135(5):1311-9.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Bioassay


3. HkRP3 is a microtubule-binding protein regulating lytic granule clustering and NK cell killing.
   Authors: Ham H, Huynh W, Schoon R, Vale R, Billadeau D
   J Immunol, 2015;194(8):3984-96.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Bioassay


4. NKG2D triggers cytotoxicity in murine epidermal gammadelta T cells via PI3K-dependent, Syk/ZAP70-independent signaling pathway.
   Authors: Ibusuki A, Kawai K, Yoshida S, Uchida Y, Nitahara-Takeuchi A, Kuroki K, Kajikawa M, Ose T, Maenaka K, Kasahara M, Kanekura T
   J Invest Dermatol, 2014;134(2):396-404.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


5. CEACAM1 on activated NK cells inhibits NKG2D-mediated cytolytic function and signaling.
   Authors: Hosomi S, Chen Z, Baker K, Chen L, Huang Y, Olszak T, Zeissig S, Wang J, Mandelboim O, Beauchemin N, Lanier L, Blumberg R
   Eur J Immunol, 2013;43(9):2473-83.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


6. Small intestinal intraepithelial lymphocytes expressing CD8 and T cell receptor gammadelta are involved in bacterial clearance during Salmonella enterica serovar Typhimurium infection.
   Authors: Li Z, Zhang C, Zhou Z, Zhang J, Zhang J, Tian Z
   Infect. Immun., 2012;80(2):565-74.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


7. CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation.
   Authors: Denoyer A, Godefroy D, Celerier I, Frugier J, Riancho L, Baudouin F, Rostene W, Baudouin C
   Mucosal Immunol, 2012;5(0):702.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Fresh


8. Recombinant mouse cytomegalovirus expressing a ligand for the NKG2D receptor is attenuated and has improved vaccine properties.
   Authors: Slavuljica I, Busche A, Babic M, Mitrovic M, Gasparovic I, Cekinovic D, Markova Car E, Pernjak Pugel E, Cikovic A, Lisnic VJ, Britt WJ, Koszinowski U, Messerle M, Krmpotic A, Jonjic S
   J. Clin. Invest., 2010;120(12):4532-45.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


9. Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells.
   Authors: Lundqvist A, Yokoyama H, Smith A, Berg M, Childs R
   Blood, 2009;113(24):6120-7.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


10. Two novel NKG2D ligands of the mouse H60 family with differential expression patterns and binding affinities to NKG2D.
    Authors: Takada A, Yoshida S, Kajikawa M, Miyatake Y, Tomaru U, Sakai M, Chiba H, Maenaka K, Kohda D, Fugo K, Kasahara M
    J. Immunol., 2008;180(3):1678-85.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Functional Assay

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