抗CXCL5抗体(Anti-CXCL5, Rat, Mouse-Mono antibody)
掲載日情報：2021/01/28 現在Webページ番号：28441
CXCL5に対する抗体(Anti-CXCL5, Rat, Mouse-Mono )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
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Anti-CXCL5, Rat, Mouse-Mono(135803) <Anti-LIX> |
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Anti-Rat LIX MAb (Clone 135803) |
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[在庫・価格 ：2025年04月26日 20時55分現在]
Anti-CXCL5, Rat, Mouse-Mono(135803) <Anti-LIX>
文献数： 1
- 商品コード：MAB543
- メーカー：RSD
- 包装：500μg
- 価格：¥122,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Rat LIX サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF543，スタンダードとして#543-RL-025を用いる。 別名：LIX クローン：135803 Genbank No： 6374 Protein Accession No： P97885.1 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Rat | 免疫動物 | Mouse | ||||
| 交差性 | Rat | 適用 | ELISA,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Rat LIX MAb (Clone 135803)
文献数： 0
- 商品コード：MAB543-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 クローン：135803 Genbank No： 6374 Protein Accession No： P97885.1 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Rat | 適用 | ELISA,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Rat |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant rat LIXAla38-Arg125, Ala38-Lys117Accession # P97885.1 |
| Source | Monoclonal Mouse IgG1 Clone # 135803 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects rat LIX in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) LIX is observed, 5% cross-reactivity with recombinant rat (rr) CXCL2 is observed, and no cross-reactivity with recombinant human rhCXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, or 13, rmCXCL1, 2, 9, 10, 11, 12, 13, or 15, recombinant porcine (rp) CXCL8, or rrCXCL1 or 3 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | Recombinant Rat LIX (Catalog # 543-RL) under non-reducing conditions only |
| Rat LIX Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Rat LIX Antibody (Catalog #MAB543 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Rat LIX Biotinylated Antibody (Catalog #BAF543 ) |
| ELISA Standard | Recombinant Rat LIX Protein (Catalog #543-RL ) | |
| Neutralization | Measured by its ability to neutralize LIX-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 0.2 µg/mL Recombinant Rat LIX. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Neutralization |
 click image to view larger | Chemotaxis Induced by LIX and Neutralization by Rat LIX Antibody.Recombinant Rat LIX (Catalog # 543-RL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat LIX (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Rat LIX Monoclonal Antibody (Catalog # MAB543). The ND50 is typically 1-5 µg/mL. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: LIX | LIX (Liposaccharide-Induced CXC chemokine; also GARG-8) is a secreted 8-10 kDa member of the Intercrine alpha (or CxC) family of chemokines. It is widely expressed, being produced by diverse cell types such as fibroblasts, thymic epithelium, platelets, vascular endothelium, hepatocytes, lung type II alveolar cells and ileal columnar epithelium. As a chemokine, LIX demonstrates chemotactic properties. It induces the chemotaxis of neutrophils and endothelial cells, and also promotes TNF-alpha secretion from mast cells and macrophages. Notably, circulating LIX is not derived from fibroblasts, but platelets. This suggests that neutrophil homeostasis/chemotaxis is a function of local resident cell activation and LIX secretion, not generally circulating LIX. Rat LIX is synthesized as a 130 amino acid (aa) precursor that contains a 37 aa signal sequence plus a 93 aa mature region (aa 38-130) that likely possesses a cleavable 13 aa C-terminus (SwissProt #:P97885). The mature region possesses a receptor-binding ELR/GluLeuArg motif between aa 47-50, and an alpha -family characteristic CxC motif between aa 50-52. Although there are no known splice variants of rat LIX, based on mouse, considerable proteolytic processing likely occurs at both the N- and C-termini over aa 38-130. This may reduce the MW in SDS-Page by as much as 3 kDa. The majority of LIX could be expected to start between aa 43-46, and this is likely to be positively correlated with bioactivity. Over aa 38-117, rat LIX shares 73% aa sequence identity with mouse LIX. Although not a strict ortholog, rat LIX also shares 64% aa sequence identity with human GCP-2.
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| Entrez Gene IDs: | 20311 (Mouse); 60665 (Rat) | |
| Alternate Names: | LIX | |
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Related Research Areas
| Chemokines and Receptors | ||
| CXC Chemokines and Receptors | ||
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| Neutralization | |
|---|---|
 | Chemotaxis Induced by LIX and Neutralization by Rat LIX Antibody. Recombinant Rat LIX (Catalog # 543-RL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat LIX (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Rat LIX Monoclonal Antibody (Catalog # MAB543). The ND50 is typically 1-5 µg/mL. |
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Related Product & Information
| Entrez Gene IDs | 20311 (Mouse); 60665 (Rat) |
|---|---|
| Background | LIX |
| background_content | Background: LIX LIX (Liposaccharide-Induced CXC chemokine; also GARG-8) is a secreted 8-10 kDa member of the Intercrine alpha (or CxC) family of chemokines. It is widely expressed, being produced by diverse cell types such as fibroblasts, thymic epithelium, platelets, vascular endothelium, hepatocytes, lung type II alveolar cells and ileal columnar epithelium. As a chemokine, LIX demonstrates chemotactic properties. It induces the chemotaxis of neutrophils and endothelial cells, and also promotes TNF-alpha secretion from mast cells and macrophages. Notably, circulating LIX is not derived from fibroblasts, but platelets. This suggests that neutrophil homeostasis/chemotaxis is a function of local resident cell activation and LIX secretion, not generally circulating LIX. Rat LIX is synthesized as a 130 amino acid (aa) precursor that contains a 37 aa signal sequence plus a 93 aa mature region (aa 38-130) that likely possesses a cleavable 13 aa C-terminus (SwissProt #:P97885). The mature region possesses a receptor-binding ELR/GluLeuArg motif between aa 47-50, and an alpha -family characteristic CxC motif between aa 50-52. Although there are no known splice variants of rat LIX, based on mouse, considerable proteolytic processing likely occurs at both the N- and C-termini over aa 38-130. This may reduce the MW in SDS-Page by as much as 3 kDa. The majority of LIX could be expected to start between aa 43-46, and this is likely to be positively correlated with bioactivity. Over aa 38-117, rat LIX shares 73% aa sequence identity with mouse LIX. Although not a strict ortholog, rat LIX also shares 64% aa sequence identity with human GCP-2. |
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Citations
- Rat coronaviruses infect rat alveolar type I epithelial cells and induce expression of CXC chemokines.
Authors: Miura TA, Wang J, Holmes KV, Mason RJ
Virology, 2007;369(2):288-98.
Species: Rat
Sample Type: Cell Culture Supernates
Application: ELISA Development
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