抗MIP-3α抗体(Anti-MIP-3α, Rat, Mouse-Mono antibody)
掲載日情報：2021/01/28 現在Webページ番号：28438
MIP-3αに対する抗体(Anti-MIP-3α, Rat, Mouse-Mono )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時35分現在]
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Anti-MIP-3α, Rat, Mouse-Mono(83225) <Anti-CCL20> |
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Anti-Rat CCL20/MIP-3 alpha MAb (Clone 83225) |
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[在庫・価格 ：2025年04月26日 20時35分現在]
Anti-MIP-3α, Rat, Mouse-Mono(83225) <Anti-CCL20>
文献数： 1
- 商品コード：MAB540
- メーカー：RSD
- 包装：500μg
- 価格：¥122,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Rat CCL20/MIP-3α サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF540，スタンダードとして#540-RM-025を用いる。 別名：beta chemokine exodus-1 クローン：83225 Genbank No： 6364 Protein Accession No： P97884.1 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Rat | 免疫動物 | Mouse | ||||
| 交差性 | Rat | 適用 | ELISA,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Rat CCL20/MIP-3 alpha MAb (Clone 83225)
文献数： 0
- 商品コード：MAB540-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：beta chemokine exodus-1 クローン：83225 Genbank No： 6364 Protein Accession No： P97884.1 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Rat | 適用 | ELISA,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Rat |
|---|---|
| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant rat CCL20/MIP-3 alpha Ala26-Met96Accession # P97884.1 |
| Source | Monoclonal Mouse IgG1 Clone # 83225 |
| Purification | Protein A or G purified from ascites |
| Specificity | Detects rat CCL20/MIP-3 alpha in ELISAs and Western blots. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | Recombinant Rat CCL20/MIP‑3 alpha (Catalog # 540-RM) under non-reducing conditions only |
| Rat CCL20/MIP-3 alpha Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Rat CCL20/MIP‑3 alpha Antibody (Catalog #MAB540 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Rat CCL20/MIP‑3 alpha Biotinylated Antibody (Catalog #BAF540 ) |
| ELISA Standard | Recombinant Rat CCL20/MIP-3 alpha Protein (Catalog #540-RM ) | |
| Neutralization | Measured by its ability to neutralize CCL20/MIP‑3 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR6. The Neutralization Dose (ND50) is typically 20-40 µg/mL in the presence of 5 µg/mL Recombinant Rat CCL20/MIP‑3 alpha. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Neutralization |
![Neutralization CCL20/MIP-3 alpha Antibody (83225) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/CCL20_MAB540_Block_Neutralize_9232.jpg) click image to view larger | Chemotaxis Induced by CCL20/MIP‑3 alpha and Neutralization by Rat CCL20/MIP‑3 alpha Antibody.Recombinant Rat CCL20/MIP‑3 alpha (Catalog # 540-RM) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CCL20/MIP‑3 alpha (5 µg/mL) is neutralized (green line) by increasing concentrations of Rat CCL20/MIP‑3 alpha Monoclonal Antibody (Catalog # MAB540). The ND50 is typically 20-40 µg/mL. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: CCL20/MIP-3 alpha | CCL20, also known as MIP-3 alpha, LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. Rat CCL20 cDNA encodes a 96 amino acid (aa) precursor protein with a 25 aa putative signal peptide that is predicted to be cleaved to form the 71 aa mature secreted protein. CCL20 is distantly related to other beta chemokines (20-28% aa sequence identity). Rat MIP-3 alpha shares approximately 70% and 61% aa sequence homology with mouse and human CCL20, respectively. CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors. |
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References
| Baba, M. et al. (1997) J. Biol. Chem. 272:14893. | |
| Hromas, R. et al. (1997) Blood 89:3315. | |
| Greaves, D.R. et al. (1997) J. Exp. Med. 186: 857. | |
| Tanaka, Y. et al. (1999) Eur. J. Immunol. 29:633. | |
| Entrez Gene IDs: | 6364 (Human); 20297 (Mouse); 29538 (Rat) |
| Alternate Names: | beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38 |
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Related Research Areas
| CC Chemokines and Receptors | ||||||||||
| Chemokines and Receptors | ||||||||||
| Chemokines and Receptors in Angiogenesis | ||||||||||
| Macrophage Activation Markers | ||||||||||
| Molecules Secreted by VSMC | ||||||||||
| Regulatory T Cells (Tregs) | ||||||||||
| Th17 Cells | ||||||||||
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| Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||||
|---|---|---|---|---|---|---|---|---|---|---|
 Enlarge | Excellent | ELISA | Rattus norvegicus | Anonymous | 01/11/2017 | Image Details |
 ELISA: Rat CCL20/MIP-3 alpha Antibody [MAB540]. | |||
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Summary
| Application | ELISA |
| Sample Tested | Rat lung epithelial cells |
| Species | Rattus norvegicus |
| Neutralization | |
|---|---|
 | Chemotaxis Induced by CCL20/MIP‑3 alpha and Neutralization by Rat CCL20/MIP‑3 alpha Antibody. Recombinant Rat CCL20/MIP‑3 alpha (Catalog # 540-RM) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CCL20/MIP‑3 alpha (5 µg/mL) is neutralized (green line) by increasing concentrations of Rat CCL20/MIP‑3 alpha Monoclonal Antibody (Catalog # MAB540). The ND50 is typically 20-40 µg/mL. |
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Related Product & Information
| Entrez Gene IDs | 6364 (Human); 20297 (Mouse); 29538 (Rat) |
|---|---|
| Background | CCL20/MIP-3 alpha |
| background_content | Background: CCL20/MIP-3 alpha CCL20, also known as MIP-3 alpha, LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. Rat CCL20 cDNA encodes a 96 amino acid (aa) precursor protein with a 25 aa putative signal peptide that is predicted to be cleaved to form the 71 aa mature secreted protein. CCL20 is distantly related to other beta chemokines (20-28% aa sequence identity). Rat MIP-3 alpha shares approximately 70% and 61% aa sequence homology with mouse and human CCL20, respectively. CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors. |
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Citations
- Aberrant cytokine secretion and zinc uptake in chronic cadmium-exposed lung epithelial cells
Authors: Andy T Y Lau
Proteomics Clin Appl, 2016;0(0):.
Species: Rat
Sample Type: Whole Cells
Application: Neut
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