抗Mouse CCL24/Eotaxin-2抗体(Anti-Mouse CCL24/Eotaxin-2 antibody)
掲載日情報:2021/01/28 現在Webページ番号:28429
Mouse CCL24/Eotaxin-2に対する抗体(Anti-Mouse CCL24/Eotaxin-2 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 :2025年04月26日 20時35分現在]
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Anti-Mouse CCL24/Eotaxin-2 MAb (Clone 106521) |
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Anti-Mouse CCL24/Eotaxin-2 MAb (Clone 106521) |
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Anti-Mouse CCL24/Eotaxin-2 MAb (Clone 106521) |
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[在庫・価格 :2025年04月26日 20時35分現在]
Anti-Mouse CCL24/Eotaxin-2 MAb (Clone 106521)
文献数: 0
- 商品コード:MAB528-100
- メーカー:RSD
- 包装:100μg
- 価格:¥97,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | 別名:chemokine (C-C motif) ligand 24 クローン:106521 Genbank No: 6369 Protein Accession No: Q9JKC0 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | 免疫動物 | Rat | |||||
交差性 | Mouse | 適用 | ELISA,Neutralising,Western Blot | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | M1/M2 Macrophage Activation Marker |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
Anti-Mouse CCL24/Eotaxin-2 MAb (Clone 106521)
文献数: 0
- 商品コード:MAB528-500
- メーカー:RSD
- 包装:500μg
- 価格:¥232,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | マッチドペア:Mouse CCL24/Eotaxin-2/MPIF-2 サンドイッチELISAの補足用抗体として利用可能,検出用抗体として#BAF528,スタンダードとして#528-MP-025を用いる。 別名:chemokine (C-C motif) ligand 24 クローン:106521 Genbank No: 6369 Protein Accession No: Q9JKC0 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | 免疫動物 | Rat | |||||
交差性 | Mouse | 適用 | ELISA,Neutralising,Western Blot | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | M1/M2 Macrophage Activation Marker |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
Anti-Mouse CCL24/Eotaxin-2 MAb (Clone 106521)
文献数: 0
- 商品コード:MAB528-SP
- メーカー:RSD
- 包装:25μg
- 価格:¥30,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | ※受注発注品。形状:溶液または凍結乾燥 別名:chemokine (C-C motif) ligand 24 クローン:106521 Genbank No: 6369 Protein Accession No: Q9JKC0 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | 免疫動物 | Rat | |||||
交差性 | Mouse | 適用 | ELISA,Neutralising,Western Blot | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | M1/M2 Macrophage Activation Marker 使いっきり抗体 |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
Species Reactivity | Mouse |
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Label | Unconjugated |
Immunogen | E. coli-derived recombinant mouse CCL24/Eotaxin‑2/MPIF‑2Ile24-Val119Accession # Q9JKC0 |
Source | Monoclonal Rat IgG2A Clone # 106521 |
Purification | Protein A or G purified from hybridoma culture supernatant |
Specificity | Detects mouse CCL24/Eotaxin‑2/MPIF‑2 in Western blots. In Western blots, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 28, recombinant mouse CCL1, 2, 3, 4, 6, 7, 9/10/MIP-1 gamma, 11, 12, 19, 20, 21, 22, 25, 28, or recombinant rat CCL20 is observed. |
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Applications and Data
Recommended Concentration | Sample | |
Western Blot | 1 µg/mL | Recombinant Mouse CCL24/Eotaxin‑2/MPIF‑2 (Catalog # 528-MP) |
Mouse CCL24/Eotaxin-2/MPIF-2 Sandwich Immunoassay | Reagent | |
ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Mouse CCL24/Eotaxin‑2/MPIF‑2 Antibody (Catalog #MAB528 ) |
ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse CCL24/Eotaxin‑2/MPIF‑2 Biotinylated Antibody (Catalog #BAF528 ) |
ELISA Standard | Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 (aa 24-119) Protein (Catalog #528-MP ) | |
Neutralization | Measured by its ability to neutralize CCL24/Eotaxin‑2/MPIF‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND50) is typically 0.06-0.3 µg/mL in the presence of 20 ng/mL Recombinant Mouse CCL24/Eotaxin‑2/MPIF‑2. | |
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. |
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Data Examples
Neutralization |
![]() click image to view larger | Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutralization by Mouse CCL24/ Eotaxin‑2 Antibody.Recombinant Mouse CCL24/ Eotaxin‑2 (Catalog # 1017-MP) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL24/Eotaxin‑2 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL24/Eotaxin‑2 Monoclonal Antibody (Catalog # MAB528). The ND50 is typically 0.06‑0.3 µg/mL. |
Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
Background: CCL24/Eotaxin-2/MPIF-2 | Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. LPS and IL-4 also differentially regulate the expression of Eotaxin-2 in monocytes and macrophages. Mouse Eotaxin-2 cDNA encodes a 119 amino acid (aa) precursor protein that shares approximately 58% aa sequence identity with human Eotaxin-2. Functionally, Eotaxin-2 is most closely related to CCL11/Eotaxin and CCL26/Eotaxin-3. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by Eotaxin. |
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Entrez Gene IDs: | 6369 (Human); 56221 (Mouse) | |
Alternate Names: | CCL24; chemokine (C-C motif) ligand 24; Ckb-6; Eosinophil chemotactic protein 2; Eotaxin-2; member 24; MPIF2; MPIF-2; MPIF2Myeloid progenitor inhibitory factor 2; MPIF-2Small-inducible cytokine A24; SCYA24CK-beta-6 |
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Related Research Areas
Airway Inflammation and Remodeling | ||
CC Chemokines and Receptors | ||
Chemokines and Receptors | ||
Chemokines and Receptors in Allergy and Asthma | ||
Eosinophils | ||
Macrophage Activation Markers | ||
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Related Product & Information
Entrez Gene IDs | 6369 (Human); 56221 (Mouse) |
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Background | CCL24/Eotaxin-2/MPIF-2 |
background_content | Background: CCL24/Eotaxin-2/MPIF-2 Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. LPS and IL-4 also differentially regulate the expression of Eotaxin-2 in monocytes and macrophages. Mouse Eotaxin-2 cDNA encodes a 119 amino acid (aa) precursor protein that shares approximately 58% aa sequence identity with human Eotaxin-2. Functionally, Eotaxin-2 is most closely related to CCL11/Eotaxin and CCL26/Eotaxin-3. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by Eotaxin. |
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Citations
- TPL-2 restricts Ccl24-dependent immunity to Heligmosomoides polygyrus
Authors: Y Kannan, LJ Entwistle, VS Pelly, J Perez-Llor, AW Walker, SC Ley, MS Wilson
PLoS Pathog., 2017;13(7):e1006536.
Species: Mouse
Sample Type: In Vivo
Application: Neut - A critical role for IRF5 in regulating allergic airway inflammation
Authors: AJ Byrne, M Weiss, SA Mathie, SA Walker, HL Eames, D Saliba, CM Lloyd, IA Udalova
Mucosal Immunol, 2017;10(3):716-726.
Species: Mouse
Sample Type: Tissue Homogenates
Application: ELISA Capture - IL-23 and Th17 cells enhance Th2-cell-mediated eosinophilic airway inflammation in mice.
Authors: Wakashin H, Hirose K, Maezawa Y, Kagami S, Suto A, Watanabe N, Saito Y, Hatano M, Tokuhisa T, Iwakura Y, Puccetti P, Iwamoto I, Nakajima H
Am. J. Respir. Crit. Care Med., 2008;178(10):1023-32.
Species: Mouse
Sample Type: In Vivo
Application: In vivo - Intranasal mite allergen induces allergic asthma-like responses in NC/Nga mice.
Authors: Shibamori M, Ogino K, Kambayashi Y, Ishiyama H
Life Sci., 2005;78(9):987-94.
Species: Mouse
Sample Type: BALF
Application: ELISA Development - Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
Authors: Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C
Immunity, 0;43(5):945-58.
Species: Mouse
Sample Type: Tissue Homogenates
Application: ELISA Capture
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